公开(公告)号：US20090081735A1

公开(公告)日：2009-03-26

申请号：US12278227

申请日：2007-02-05

申请人： David Martin Anderson ,  Andrew John Collis ,  Lin Liu ,  Sergey Podkovyrov ,  Christopher Preston

发明人： David Martin Anderson ,  Andrew John Collis ,  Lin Liu ,  Sergey Podkovyrov ,  Christopher Preston

IPC分类号： C12P19/38 ,  C12N1/21

CPC分类号： C12N15/52 ,  C12P19/38 ,  C12P19/385

摘要： Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e.g. T4 frd; Serine Hydroxymethyltransferase genes e.g. glyA; 3-phosphoglycerate dehydrogenase genes e.g. serA; and THF synthase genes e.g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine.

摘要翻译： 公开了包括DNA构建宿主细胞组合在内的新生物。 生物体包含转录单元（例如操纵子），其包含编码酶的DNA序列，其促进用于将dUMP转化成dTMP的单个碳单元的供应。 二氢叶酸还原酶基因例如： T4 frd; 丝氨酸羟甲基转移酶基因例如 glyA; 3-磷酸​​甘油酸脱氢酶基因 血清学 和THF合酶基因例如。 ADE3。 生物用于生产胸苷的生物学方法，尿苷水平显着降低。

公开(公告)号：US20050009083A1

公开(公告)日：2005-01-13

申请号：US10914529

申请日：2004-08-09

申请人： David Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

发明人： David Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

IPC分类号： C12N15/09 ,  C12N1/16 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/78 ,  C12N15/52 ,  C12N15/53 ,  C12N15/54 ,  C12N15/55 ,  C12P19/38 ,  C12R1/19 ,  C12R1/86 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/22

CPC分类号： C12N9/0036 ,  C12N9/0093 ,  C12N9/1007 ,  C12N9/1205 ,  C12N9/78 ,  C12N15/52 ,  C12P19/385

摘要： Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.

摘要翻译： 公开了新的DNA构建体和包含其的宿主细胞。 DNA构建体包含包含编码核糖核苷酸还原酶和硫氧还蛋白或尿苷激酶基因和/或dCTP脱氨酶基因的DNA序列的转录单位（例如操纵子）。 在优选实施方案中，包含编码核糖核苷酸还原酶和硫氧还蛋白的DNA序列的构建体还包含编码胸苷酸合酶的DNA序列和/或包含编码尿苷激酶的序列的转录单元，优选与dCTP脱氨酶一起。 在特别优选的实施方案中，宿主细胞包含具有所有上述特征的构建体，其中宿主细胞显示被抑制或没有尿嘧啶DNA糖基化酶活性。 这可以通过去除宿主细胞ung基因来实现。 宿主细胞在制造嘧啶脱氧核糖核苷酸中的用途，例如 还公开了胸苷。

公开(公告)号：US08263384B2

公开(公告)日：2012-09-11

申请号：US12278227

申请日：2007-02-05

申请人： David Martin Anderson ,  Andrew John Collis ,  Lin Liu ,  Sergey Podkovyrov ,  Christopher Preston

发明人： David Martin Anderson ,  Andrew John Collis ,  Lin Liu ,  Sergey Podkovyrov ,  Christopher Preston

IPC分类号： C12N1/21 ,  C12P19/38

CPC分类号： C12N15/52 ,  C12P19/38 ,  C12P19/385

摘要： Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e.g. T4 frd; Serine Hydroxymethyltransferase genes e.g. glyA; 3-phosphoglycerate dehydrogenase genes e.g. serA; and THF synthase genes e.g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine.

摘要翻译： 公开了包括DNA构建宿主细胞组合在内的新生物。 生物体包含转录单元（例如操纵子），其包含编码酶的DNA序列，其促进用于将dUMP转化成dTMP的单个碳单元的供应。 二氢叶酸还原酶基因例如： T4 frd; 丝氨酸羟甲基转移酶基因例如 glyA; 3-磷酸​​甘油酸脱氢酶基因 血清学 和THF合酶基因例如。 ADE3。 生物用于生产胸苷的生物学方法，尿苷水平显着降低。

公开(公告)号：US07387893B2

公开(公告)日：2008-06-17

申请号：US10914529

申请日：2004-08-09

申请人： David Martin Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

发明人： David Martin Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

IPC分类号： C12N15/00 ,  C12N1/20 ,  C12N5/10 ,  C07H21/00 ,  C12N9/02 ,  C12N9/12 ,  C12N9/78 ,  C12Q1/26 ,  C12P21/06 ,  C07K14/00

CPC分类号： C12N9/0036 ,  C12N9/0093 ,  C12N9/1007 ,  C12N9/1205 ,  C12N9/78 ,  C12N15/52 ,  C12P19/385

摘要： Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.

摘要翻译： 公开了新的DNA构建体和包含其的宿主细胞。 DNA构建体包含包含编码核糖核苷酸还原酶和硫氧还蛋白或尿苷激酶基因和/或dCTP脱氨酶基因的DNA序列的转录单位（例如操纵子）。 在优选实施方案中，包含编码核糖核苷酸还原酶和硫氧还蛋白的DNA序列的构建体还包含编码胸苷酸合酶的DNA序列和/或包含编码尿苷激酶的序列的转录单元，优选与dCTP脱氨酶一起。 在特别优选的实施方案中，宿主细胞包含具有所有上述特征的构建体，其中宿主细胞显示被抑制或没有尿嘧啶DNA糖基化酶活性。 这可以通过去除宿主细胞ung基因来实现。 宿主细胞在制造嘧啶脱氧核糖核苷酸中的用途，例如 还公开了胸苷。

公开(公告)号：US06777209B1

公开(公告)日：2004-08-17

申请号：US10018878

申请日：2002-03-06

申请人： David Martin Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

发明人： David Martin Anderson ,  Lin Liu ,  Sergey Podkovyrov ,  Baomin Wang

IPC分类号： C12N120

CPC分类号： C12N9/0036 ,  C12N9/0093 ,  C12N9/1007 ,  C12N9/1205 ,  C12N9/78 ,  C12N15/52 ,  C12P19/385

摘要： Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.

摘要翻译： 公开了新的DNA构建体和包含其的宿主细胞。 DNA构建体包含包含编码核糖核苷酸还原酶和硫氧还蛋白或尿苷激酶基因和/或dCTP脱氨酶基因的DNA序列的转录单位（例如操纵子）。 在优选实施方案中，包含编码核糖核苷酸还原酶和硫氧还蛋白的DNA序列的构建体还包含编码胸苷酸合酶的DNA序列和/或包含编码尿苷激酶的序列的转录单元，优选与dCTP脱氨酶一起。 在特别优选的实施方案中，宿主细胞包含具有所有上述特征的构建体，其中宿主细胞显示被抑制或没有尿嘧啶DNA糖基化酶活性。 这可以通过去除宿主细胞ung基因来实现。 宿主细胞在制造嘧啶脱氧核糖核苷酸中的用途，例如 还公开了胸苷。