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    Method and compositions for the production of transgenic plants
    111.
    发明授权
    Method and compositions for the production of transgenic plants 有权
    用于生产转基因植物的方法和组合物

    公开(公告)号：US07807866B2

    公开(公告)日：2010-10-05

    申请号：US10178919

    申请日：2002-06-25

    Applicant: Ahmad Parveez Ghulam Kadir

    Inventor： Ahmad Parveez Ghulam Kadir

    IPC: C12N15/82 ,  A01H5/00

    CPC classification number: A01H4/005 ,  A01H4/008 ,  C12N15/8201 ,  C12N15/8209

    Abstract: The present invention relates generally to a process of producing transgenic plants and more particularly transgenic oil palm plants carrying exogenous genetic material which confers on said plants or cells of said plants particular phenotypic traits. The present invention further provides plant parts, reproductive material and plant products from the transgenic plants. The present invention particularly provides transgenic plants and even more particularly transgenic oil plants having beneficial and useful phenotypic characteristics not present at least to the same extent in non-transgenic plants of the same species. The present invention is further directed to oil, and particularly palm oil, having beneficial and desirable characteristics, produced by the transgenic plants made in accordance with the present invention.

    Abstract translation: 本发明一般涉及产生转基因植物的方法，更特别地涉及携带外源遗传物质的转基因油棕榈植物，其赋予所述植物的特定表型性状的所述植物或细胞。 本发明还提供来自转基因植物的植物部分，繁殖材料和植物产物。 本发明特别提供了转基因植物，甚至更特别的具有在相同物种的非转基因植物中至少具有相同程度的有益和有用的表型特征的转基因植物油。 本发明进一步涉及由根据本发明制备的转基因植物产生的具有有益和期望特征的油，特别是棕榈油。

    Method Of Excising A Nucleic Acid Sequence From A Plant Genome
    113.
    发明申请
    Method Of Excising A Nucleic Acid Sequence From A Plant Genome 审中-公开
    从植物基因组中切除核酸序列的方法

    公开(公告)号：US20100162428A1

    公开(公告)日：2010-06-24

    申请号：US12600902

    申请日：2008-05-30

    Applicant: Jeffrey A. Brown ,  Fang-Ming Lai ,  Christina E. Roche ,  Hee-Sook Song

    Inventor： Jeffrey A. Brown ,  Fang-Ming Lai ,  Christina E. Roche ,  Hee-Sook Song

    IPC: A01H1/00 ,  A01H5/00

    CPC classification number: C12N15/8201 ,  C12N9/22

    Abstract: The present invention relates to a method for excising a nucleic acid sequence from the genome of a plant or a plant cell. This method is based on the steps of transforming a plant cell with a construct encoding a DNA double strand break inducing enzyme (DSBI), generating a transgenic plant line, performing a transient assay to analyze the functionality of the transgenic enzyme, crossing the plant line with a line containing a nucleic acid sequence to be excised and performing an immature embryo conversion or a tissue culture regeneration through callus formation. The method can also be reversed, which means that a plant cell is transformed with a construct encoding a nucleic acid sequence to be excised, and the crossing is performed with a plant line containing a DSBI. As an alternative to the crossing step, a re-transformation of a transgenic plant line with a second construct can also be performed. The invention is also directed to a plant obtained by this method, or progeny, propagation material, part, tissue, cell or cell culture, derived from such a plant. Finally, the invention relates to the use of a plant or progeny, propagation material, part, tissue, cell or cell culture, derived from this method, as aliment, fodder or seeds or for the production of pharmaceuticals or chemicals.

    Abstract translation: 本发明涉及从植物或植物细胞的基因组中切除核酸序列的方法。 该方法基于用编码DNA双链断裂诱导酶（DSBI）的构建体转化植物细胞的步骤，产生转基因植物系，进行瞬时测定以分析转基因酶的功能，穿过植物系 含有要切除的核酸序列的线，并通过愈伤组织形成进行未成熟胚转化或组织培养再生。 该方法也可以相反，这意味着用编码待切除的核酸序列的构建体转化植物细胞，并且用含有DSBI的植物系进行杂交。 作为交叉步骤的替代方案，也可以进行具有第二构建体的转基因植物系的再转化。 本发明还涉及通过该方法获得的植物，或从这种植物衍生的后代，繁殖材料，部分，组织，细胞或细胞培养物。 最后，本发明涉及来源于该方法的植物或后代，繁殖材料，部分，组织，细胞或细胞培养物作为营养物，饲料或种子或用于生产药物或化学品的用途。

    In Vitro Methods for the Induction and Maintenance of Plant Cell Lines as Single Suspension Cells With Intact Cell Walls, and Transformation Thereof
    114.
    发明申请
    In Vitro Methods for the Induction and Maintenance of Plant Cell Lines as Single Suspension Cells With Intact Cell Walls, and Transformation Thereof 有权
    植物细胞系诱导和维持作为具有完整细胞壁的单一悬浮细胞的体外方法及其转化

    公开(公告)号：US20100159598A1

    公开(公告)日：2010-06-24

    申请号：US11965543

    申请日：2007-12-27

    Applicant: P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garrison ,  William M. Ainley ,  Narasimha C. Samboju

    Inventor： P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garrison ,  William M. Ainley ,  Narasimha C. Samboju

    IPC: C12N15/82 ,  C12N5/04 ,  C12N5/10

    CPC classification number: C12N5/04 ,  C12N5/0025 ,  C12N15/8201

    Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

    Abstract translation: 本发明提供简单且一致的方法来将悬浮细胞聚集体破碎成具有完整原始细胞壁的单个细胞。 本发明部分涉及在包含果胶降解酶或包含秋水仙素的微管蛋白去聚合化合物的培养基中培养的悬浮细胞聚集体的细胞分离。 本发明还涉及化合物用于这些目的的新用途。 本发明的另一方面涉及受试者，分离的细胞的转化。 这样的过程简化并将基于单细胞的转化和选择过程整合到转基因和转基因组事件生成工作过程中。 本发明还消除了技术限制，并以高通量方式产生无标记和均匀表达的转基因品系，以支持动物健康，生物制药和性状和作物保护平台的各种需要。

    Processes and vectors for producing transgenic plants
    115.
    发明授权
    Processes and vectors for producing transgenic plants 有权
    用于生产转基因植物的方法和载体

    公开(公告)号：US07652194B2

    公开(公告)日：2010-01-26

    申请号：US10416931

    申请日：2001-12-07

    Applicant: Yuri Gleba ,  Victor Klimyuk ,  Gregor Benning ,  Serik Eliby

    Inventor： Yuri Gleba ,  Victor Klimyuk ,  Gregor Benning ,  Serik Eliby

    IPC: C12N15/82 ,  C12N15/40

    CPC classification number: C12N15/8213 ,  C12N15/8201 ,  C12N15/8216 ,  C12N15/8257 ,  C12N15/8274

    Abstract: This invention describes a process for gene expression in plants utilizing translational vectors. Said translational vectors cause a gene of interest to be stably integrated into a transcriptionally active host genomic DNA such that the transcription of the gene of interest is controlled by a promoter of the host plant. Said translational vectors are preferably based on internal ribosome entry site (IRES) elements that are of plant origin.

    Abstract translation: 本发明描述了利用翻译载体在植物中表达基因的方法。 所述翻译载体使感兴趣的基因稳定地整合到转录活性宿主基因组DNA中，使得感兴趣的基因的转录由宿主植物的启动子控制。 所述翻译载体基于植物来源的内部核糖体进入位点（IRES）元件。

    Transformation of immature soybean seeds through organogenesis
    120.
    发明申请
    Transformation of immature soybean seeds through organogenesis 审中-公开
    通过器官发生转化未成熟大豆种子

    公开(公告)号：US20080229447A1

    公开(公告)日：2008-09-18

    申请号：US11716975

    申请日：2007-03-12

    Applicant: YuehJiang Hwang ,  John Dawson ,  Marina Sigareva ,  Qiudeng Que

    Inventor： YuehJiang Hwang ,  John Dawson ,  Marina Sigareva ,  Qiudeng Que

    IPC: A01H5/00 ,  C12N15/87 ,  C12N5/04

    CPC classification number: C12N15/8205 ,  A01H4/005 ,  C12N15/8201

    Abstract: The present disclosure provides methods for the transformation of soybean cells or tissue and regeneration of the soybean cells or tissue into transformed plants. The disclosed methods utilize an explant prepared from an immature soybean seedling which can be induced directly to form shoots that give rise to transgenic plants via organogenesis. The disclosed methods do not require germination and are rapid and efficient.

    Abstract translation: 本公开提供了用于转化大豆细胞或组织以及将大豆细胞或组织再生成转化植物的方法。 所公开的方法利用由未成熟大豆幼苗制备的外植体，其可以直接诱导形成通过器官发生产生转基因植物的芽。 所公开的方法不需要发芽并且是快速和有效的。

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