METHOD OF CO-CULTURING MAMMALIAN MUSCLE CELLS AND MOTONEURONS

    公开(公告)号:US20170313980A1

    公开(公告)日:2017-11-02

    申请号:US15594895

    申请日:2017-05-15

    Abstract: The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.

    Photoacid compositions having extended lifetime of proton dissociation state

    公开(公告)号:US09708257B2

    公开(公告)日:2017-07-18

    申请号:US13748253

    申请日:2013-01-23

    Inventor: Yi Liao

    CPC classification number: C07D209/12

    Abstract: A photoacid includes a nucleophilic (NuH) moiety having a photodissociable proton, an electron accepting (EA) moiety, and a bridge structure (X) bonded to both the NuH moiety and EA moiety positioned between the NuH and EA moieties. The NuH and EA moieties each include respective structure so that the EA moiety bonds to a proton photodissociated form of the NuH moiety during a reversible photoinduced intramolecular reaction to form a ring, which has been found to significantly increase the lifetime of the proton dissociation state.

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