公开(公告)号：US11833192B2

公开(公告)日：2023-12-05

申请号：US17494733

申请日：2021-10-05

申请人： AMANO ENZYME INC. ,  HIROSHIMA UNIVERSITY

发明人： Manabu Kuroda ,  Shotaro Yamaguchi ,  Norihisa Kato

IPC分类号： C12N9/62 ,  A61K38/48 ,  A61P1/14 ,  A23L33/17 ,  A61P1/10 ,  A61K9/00 ,  A61K38/46 ,  A23L2/52 ,  A61K36/062 ,  C12N9/50 ,  A23L33/10 ,  A23L29/00 ,  A23K50/30 ,  C12N9/26 ,  C12N9/24

CPC分类号： A61K38/46 ,  A23K50/30 ,  A23L2/52 ,  A23L29/06 ,  A23L33/10 ,  A23L33/17 ,  A61K9/0053 ,  A61K36/062 ,  A61K38/48 ,  A61K38/4806 ,  A61P1/10 ,  A61P1/14 ,  C12N9/248 ,  C12N9/2411 ,  C12N9/50 ,  C12N9/62 ,  A01K2227/108 ,  A23V2002/00 ,  A23V2002/00 ,  A23V2200/32 ,  A23V2250/208

摘要： A method for improving intestinal flora increases the number of beneficial bacteria such as lactic acid bacteria and Bifidobacterium to improve intestinal flora by using an enzyme. The method involves providing a protease, which is a polypeptide having an amino acid sequence shown in SEQ ID NO: 1 that can increase beneficial bacteria such as lactic acid bacteria and Bifidobacterium in intestines to exert an excellent effect of improving intestinal flora.

公开(公告)号：US20230329262A1

公开(公告)日：2023-10-19

申请号：US18245627

申请日：2021-09-16

申请人： AMANO ENZYME INC.

发明人： Ryosuke TANAKA

IPC分类号： A23C11/10 ,  A23L27/00 ,  A23L29/00

CPC分类号： A23C11/103 ,  A23L27/88 ,  A23L29/06

摘要： The purpose of the present invention is to provide a processing technique whereby the flavor of a chickpea milk can be improved. According to this method of manufacturing a processed chickpea milk including a step for treating a chickpea milk with a protein deamidase, the flavor of the chickpea milk can be improved.

公开(公告)号：US11718837B2

公开(公告)日：2023-08-08

申请号：US17255831

申请日：2020-07-29

申请人： AMANO ENZYME INC.

发明人： Tomohiro Kimura ,  Tomoya Ryoki ,  Yusuke Tanaka ,  Naoto Katsumata ,  Yukihide Sato

IPC分类号： C12N9/20 ,  C12P7/64 ,  C12P7/6454

CPC分类号： C12N9/20 ,  C12P7/64 ,  C12P7/6454 ,  C12Y301/01003

摘要： To find a novel lipase suitable for processing of oils and fats and to use and apply the lipase. Provided are a novel lipase including an amino acid sequence having 90% or more identity with an amino acid sequence of SEQ ID NO: 1 or 2, and an enzyme preparation containing the same as an active ingredient. The enzyme preparation is used for modifying and improving the physical properties of oils and fats.

公开(公告)号：US20230240312A1

公开(公告)日：2023-08-03

申请号：US18042515

申请日：2021-08-24

申请人： AMANO ENZYME INC. ,  AMANO ENZYME EUROPE LTD.

发明人： Hiroki FUJIOKA

IPC分类号： A23C11/10 ,  A23F5/46 ,  A23F3/40 ,  A23L23/00 ,  C12N9/80

CPC分类号： A23C11/103 ,  A23F5/465 ,  A23F3/405 ,  A23L23/00 ,  C12N9/80 ,  C12Y305/01044

摘要： The present invention addresses the problem of creating an effective means for preventing the coagulation of a plant milk under high temperature conditions. To solve this problem, a plant milk is treated with a protein deamidase to thereby prevent the coagulation of the plant milk in the case of adding to a hot liquid beverage, a hot liquid food, etc.

公开(公告)号：US20230125821A1

公开(公告)日：2023-04-27

申请号：US17908761

申请日：2021-02-26

申请人： Amano Enzyme Inc.

发明人： Kazunori Yoshida

IPC分类号： C12N9/10

摘要： The present invention addresses the issues of finding a novel mutation effective for the improvement of transglutaminase and providing a highly useful modified transglutaminase. Disclosed is a highly useful modified transglutaminase having an amino acid substitution that results in an increase of high temperature reactivity or a lowering in pH stability in a weakly acidic region.

公开(公告)号：US20230087917A1

公开(公告)日：2023-03-23

申请号：US17904998

申请日：2021-02-26

申请人： Amano Enzyme Inc.

发明人： Yuki Ishigaki

IPC分类号： C12N9/62 ,  A61K36/062 ,  A23L33/10 ,  A23L31/00 ,  A23L29/00

摘要： Provided is a digestive enzyme agent which can promote the liberation of a protein into BCAAs in an in vivo environment. A digestive enzyme agent comprising a protease derived from a koji mold can promote the liberation into BCAAs in an in vivo environment.

公开(公告)号：US11555214B2

公开(公告)日：2023-01-17

申请号：US16574149

申请日：2019-09-18

申请人： AMANO ENZYME INC.

发明人： Genichi Nakamura ,  Yuko Kuritani ,  Satoru Ishihara ,  Tetsuya Takahashi

IPC分类号： C12N9/38 ,  C12P19/14 ,  C12P19/04 ,  C12P19/12

摘要： The usefulness of β-galactosidases derived from Bacillus circulans is further enhanced. A modified β-galactosidase in which one or more amino acids selected from the group consisting of proline 182 (P182), tyrosine 187 (Y187), serine 188 (S188), tryptophan 405 (W405), alanine 406 (A406), glutamine 407 (Q407), tyrosine 449 (Y449), threonine 483 (T483), serine 512 (S512), serine 531 (S531), threonine 533 (T533), serine 534 (S534), asparagine 550 (N550), glutamine 551 (Q551), tryptophan 593 (W593), tyrosine 598 (Y598), proline 602 (P602), proline 604 (P604), tyrosine 609 (Y609), lysine 612 (K612), and tyrosine 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a β-galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.

公开(公告)号：US20230002750A1

公开(公告)日：2023-01-05

申请号：US17782531

申请日：2020-12-03

申请人： AMANO ENZYME INC. ,  JICHI MEDICAL UNIVERSITY

发明人： Kazuhiro FURUKAWA ,  Yuki ISHIGAKI ,  Kotaro YOSHIMURA ,  Takako SHIRADO ,  Natsumi SAITO ,  Rintaro ASAHI ,  Masanori MORI

IPC分类号： C12N9/52 ,  C12N5/071 ,  C12N5/077

摘要： A method for preparing a stromal vascular fraction from an adipose tissue, including a step of treating an adipose tissue with an enzyme solution containing a collagenase and a neutral protease, preferably an enzyme solution free of clostripain and thermolysin, and showing not less than 1 U neutral protease activity with respect to 10,000 U collagenase activity, and recovering cells is provided by the present invention.

公开(公告)号：US20220346423A1

公开(公告)日：2022-11-03

申请号：US17765232

申请日：2020-10-02

申请人： Amano Enzyme Inc. ,  Amano Enzyme U.S.A. Co., Ltd. ,  Amano Enzyme Europe Ltd.

发明人： Keita Okuda ,  Hiroki Fujioka

IPC分类号： A23L31/15 ,  C12N9/22

摘要： It is an object of the present invention to achieve a simplified process of producing a yeast extract, improved production efficiency, reduced production costs, and the like. A yeast cell lysate is treated with a ribonuclease and AMP-deaminase simultaneously to improve production efficiency. Use of heat-resistant AMP-deaminase allows reactions with the ribonuclease and the AMP-deaminase to proceed simultaneously in an efficient manner.

公开(公告)号：US20220338503A1

公开(公告)日：2022-10-27

申请号：US17753620

申请日：2020-09-10

申请人： Amano Enzyme Inc. ,  Amano Enzyme U.S.A. Co., Ltd.

发明人： Keita Okuda

IPC分类号： A23J1/14 ,  A23L11/60 ,  A23L2/66 ,  A23L29/00 ,  A23L3/40 ,  C12P21/00

摘要： The present invention addresses the problem of providing a means for efficiently producing a plant protein concentrate to be utilized in foods, beverages, etc. The present invention also addresses the problem of raising the utility value of plant proteins and contributing to the improvement of quality in existing uses and the discovery of new uses by making it possible to produce a plant protein concentrate having improved physical properties (especially solubility). The yield of plant protein is improved by treating a plant protein raw material such as peas, soybeans, almonds, etc., with a protein deamidase.