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公开(公告)号：US20070166752A1
公开(公告)日：2007-07-19
申请号：US11688071
申请日：2007-03-19
Applicant: Scott Weber , Derek Douglas , Carol Kreader
Inventor： Scott Weber , Derek Douglas , Carol Kreader
CPC classification number: C12N15/1003
Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
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12.发明申请Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography 有权使用碳水化合物非离子型去垢剂与二氧化硅色谱法在质粒制备物中还原内毒素的方法公开(公告)号：US20050245733A1
公开(公告)日：2005-11-03
申请号：US11108317
申请日：2005-04-18
Applicant: Kevin Ray , Carol Kreader , Fuqiang Chen , David Cutter
Inventor： Kevin Ray , Carol Kreader , Fuqiang Chen , David Cutter
CPC classification number: C07H21/04 , C12N15/101
Abstract: The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.
Abstract translation: 本发明提供使用碳水化合物非离子洗涤剂与二氧化硅色谱法还原质粒制备中的内毒素的方法。
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13.发明授权公开(公告)号：US09062303B2
公开(公告)日：2015-06-23
申请号：US12820250
申请日：2010-06-22
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
Abstract translation: 本发明提供了从生物样品中快速和有效地分离小RNA的方法。 特别地，将小RNA从生物样品中的大RNA，DNA，蛋白质和其他大分子中分离和分离。
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Patent Agency Ranking
14.发明申请公开(公告)号：US20100256351A1
公开(公告)日：2010-10-07
申请号：US12820250
申请日：2010-06-22
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
IPC: C07H21/02
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
Abstract translation: 本发明提供了从生物样品中快速和有效地分离小RNA的方法。 特别地，将小RNA从生物样品中的大RNA，DNA，蛋白质和其他大分子中分离和分离。
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公开(公告)号：US20080026375A1
公开(公告)日：2008-01-31
申请号：US11461273
申请日：2006-07-31
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: B01J45/00 , B01J20/286 , B01J39/26 , B01J41/20 , G01N30/96
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
Abstract translation: 本发明提供带电分离介质，包含分离介质的试剂盒，以及使用该分离介质结合并任选分离生物分子的方法。 带电分离介质和方法利用非共价结合分离介质的聚离子将期望的生物分子结合到分离介质。
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16.发明申请公开(公告)号：US20070202511A1
公开(公告)日：2007-08-30
申请号：US11363982
申请日：2006-02-28
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides extraction compositions and methods for the rapid and efficient isolation of small RNA molecules from a biological sample. In particular, the extraction compositions, when contacted with a biological sample, releases the small RNA molecules from the other molecules in a biological sample, and the released small RNA molecules may then be isolated.
Abstract translation: 本发明提供了用于从生物样品中快速和有效地分离小RNA分子的提取组合物和方法。 特别地，当与生物样品接触时，提取组合物从生物样品中的其他分子释放小RNA分子，然后可以分离释放的小RNA分子。
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公开(公告)号：US20070172874A1
公开(公告)日：2007-07-26
申请号：US11687999
申请日：2007-03-19
Applicant: Scott Weber , Derek Douglas , Carol Kreader
Inventor： Scott Weber , Derek Douglas , Carol Kreader
CPC classification number: C12N15/1003
Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
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18.发明授权Use of an alcohol-salt buffer wash for the efficient recovery of mycobacteria and mycobacterial DNA from respiratory sediment 失效使用酒精盐缓冲液洗涤有效地从呼吸沉积物回收分枝杆菌和分枝杆菌DNA
公开(公告)号：US6126839A
公开(公告)日：2000-10-03
申请号：US13987
申请日：1998-01-27
Applicant: Carol Kreader , John W. Backus , Joanne H. Kerschner , Rashmi Mehta
Inventor： Carol Kreader , John W. Backus , Joanne H. Kerschner , Rashmi Mehta
IPC: G01N33/48 , C12N1/00 , C12N15/00 , C12N15/09 , C12Q1/04 , C12Q1/68 , C12R1/32 , G01N33/50 , B01D21/26 , B01D21/01
Abstract: The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of 0.7 to 0.9 g/ml and a boiling point greater than 50.degree. C. The invention also relates to methods for concentrating bacteria and free bacterial nucleic acids from a biological sample that involve mixing with the sample a density-lowering agent and a monovalent salt.
Abstract translation: 本发明涉及浓缩来自粘性生物样品的细菌的方法。 该方法包括向样品中加入密度为0.7至0.9g / ml且沸点大于50℃的水溶性密度降低剂。本发明还涉及浓缩细菌和游离细菌核酸的方法 从涉及与样品混合的生物样品中降低密度的剂和单价盐。
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