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公开(公告)号：US20080023395A1
公开(公告)日：2008-01-31
申请号：US11461235
申请日：2006-07-31
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
IPC: B01D39/00
CPC classification number: C12N15/101
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
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公开(公告)号：US20080026374A1
公开(公告)日：2008-01-31
申请号：US11461263
申请日：2006-07-31
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12Q1/6813
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
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3.发明申请Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography 有权使用碳水化合物非离子型去垢剂与二氧化硅色谱法在质粒制备物中还原内毒素的方法公开(公告)号：US20050245733A1
公开(公告)日：2005-11-03
申请号：US11108317
申请日：2005-04-18
Applicant: Kevin Ray , Carol Kreader , Fuqiang Chen , David Cutter
Inventor： Kevin Ray , Carol Kreader , Fuqiang Chen , David Cutter
CPC classification number: C07H21/04 , C12N15/101
Abstract: The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.
Abstract translation: 本发明提供使用碳水化合物非离子洗涤剂与二氧化硅色谱法还原质粒制备中的内毒素的方法。
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4.发明授权公开(公告)号：US09062303B2
公开(公告)日：2015-06-23
申请号：US12820250
申请日：2010-06-22
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
Abstract translation: 本发明提供了从生物样品中快速和有效地分离小RNA的方法。 特别地，将小RNA从生物样品中的大RNA，DNA，蛋白质和其他大分子中分离和分离。
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5.发明申请公开(公告)号：US20100256351A1
公开(公告)日：2010-10-07
申请号：US12820250
申请日：2010-06-22
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
IPC: C07H21/02
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
Abstract translation: 本发明提供了从生物样品中快速和有效地分离小RNA的方法。 特别地，将小RNA从生物样品中的大RNA，DNA，蛋白质和其他大分子中分离和分离。
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Patent Agency Ranking
公开(公告)号：US20080026375A1
公开(公告)日：2008-01-31
申请号：US11461273
申请日：2006-07-31
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: B01J45/00 , B01J20/286 , B01J39/26 , B01J41/20 , G01N30/96
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
Abstract translation: 本发明提供带电分离介质，包含分离介质的试剂盒，以及使用该分离介质结合并任选分离生物分子的方法。 带电分离介质和方法利用非共价结合分离介质的聚离子将期望的生物分子结合到分离介质。
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7.发明申请公开(公告)号：US20070202511A1
公开(公告)日：2007-08-30
申请号：US11363982
申请日：2006-02-28
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides extraction compositions and methods for the rapid and efficient isolation of small RNA molecules from a biological sample. In particular, the extraction compositions, when contacted with a biological sample, releases the small RNA molecules from the other molecules in a biological sample, and the released small RNA molecules may then be isolated.
Abstract translation: 本发明提供了用于从生物样品中快速和有效地分离小RNA分子的提取组合物和方法。 特别地，当与生物样品接触时，提取组合物从生物样品中的其他分子释放小RNA分子，然后可以分离释放的小RNA分子。
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公开(公告)号：US20070166750A1
公开(公告)日：2007-07-19
申请号：US11687960
申请日：2007-03-19
Applicant: Scott Weber , Derek Douglas , Carol Kreader
Inventor： Scott Weber , Derek Douglas , Carol Kreader
CPC classification number: C12N15/1003
Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
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公开(公告)号：US20150079657A1
公开(公告)日：2015-03-19
申请号：US14381381
申请日：2013-02-28
Applicant: Qingzhou Ji , Carol Kreader
Inventor： Qingzhou Ji , Carol Kreader
Abstract: The present disclosure provides compositions and methods for acetylating histones at targeted chromosomal locations in a cell. In particular, the disclosure provides a fusion protein comprising a DNA binding domain and at least one histone acetyltransferase (HAT) domain, such that the DNA binding domain targets the fusion protein to a targeted chromosomal location and the HAT domain acetylates histones at the targeted location.
Abstract translation: 本公开提供了在细胞中靶向染色体位置处乙酰化组蛋白的组合物和方法。 具体地，本公开提供了包含DNA结合结构域和至少一种组蛋白乙酰转移酶（HAT）结构域的融合蛋白，使得DNA结合结构域将融合蛋白靶向靶向染色体位置，并且HAT结构域在靶位置乙酰化组蛋白 。
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10.发明申请REAL TIME POLYMERASE CHAIN REACTION PROCESS USING A UNIVERSAL DETECTION SYSTEM 审中-公开使用通用检测系统的实时聚合酶链反应过程公开(公告)号：US20110136118A1
公开(公告)日：2011-06-09
申请号：US12996403
申请日：2009-06-16
Applicant: Carol Kreader , Brian Ward
Inventor： Carol Kreader , Brian Ward
IPC: C12Q1/68
CPC classification number: C12Q1/6823 , C12Q1/6851 , C12Q2561/113 , C12Q2561/109 , C12Q2537/149 , C12Q2521/301 , C12Q2565/1015 , C12Q2525/161 , C12Q2525/131
Abstract: Provided are methods and kits for detecting amplification of a target nucleic acid during a real time quantitative polymerase chain reaction process using a universal detection system. The detection system uses an unlabeled probe that detects the amplified target nucleic acid and interacts with a universal detection module.
Abstract translation: 提供了用于在使用通用检测系统的实时定量聚合酶链反应过程中检测靶核酸的扩增的方法和试剂盒。 检测系统使用检测扩增的靶核酸并与通用检测模块相互作用的未标记的探针。
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