CRISPR-BASED GENOME MODIFICATION AND REGULATION
    1.
    发明申请
    CRISPR-BASED GENOME MODIFICATION AND REGULATION 审中-公开
    基于CRISPR的基因改变和调控

    公开(公告)号:US20140273230A1

    公开(公告)日:2014-09-18

    申请号:US14213895

    申请日:2014-03-14

    CPC classification number: C12N15/85 C07K2319/09 C12N9/22 C12N15/902

    Abstract: The present invention provides methods for modifying chromosomal sequences. In particular, methods are provided for using RNA-guided endonucleases or modified RNA-guided endonucleases to modify targeted chromosomal sequences.

    Abstract translation: 本发明提供了修饰染色体序列的方法。 特别地,提供了使用RNA引导的内切核酸酶或修饰的RNA引导的内切核酸酶来修饰靶向染色体序列的方法。

    DETECTION OF MATURE SMALL RNA MOLECULES
    3.
    发明申请
    DETECTION OF MATURE SMALL RNA MOLECULES 审中-公开
    成熟小RNA分子的检测

    公开(公告)号:US20080194416A1

    公开(公告)日:2008-08-14

    申请号:US11672591

    申请日:2007-02-08

    Applicant: Fuqiang Chen

    Inventor: Fuqiang Chen

    CPC classification number: C12Q1/6816 C12Q2561/125 C12Q2525/207

    Abstract: Methods, compositions, and kits for detecting mature small RNAs are provided herein. The methods comprise ligating at least one linker to a mature small RNA in the presence of a complementary ligation template to generate a ligation product. The ligation product is a hybrid molecule comprising the linker and the mature small RNA.

    Abstract translation: 本文提供了用于检测成熟小RNA的方法,组合物和试剂盒。 所述方法包括在互补连接模板存在下将至少一个接头连接至成熟小RNA以产生连接产物。 连接产物是包含接头和成熟小RNA的杂合分子。

    UNIVERSAL LIGATION ARRAY FOR ANALYZING GENE EXPRESSION OR GENOMIC VARIATIONS
    8.
    发明申请
    UNIVERSAL LIGATION ARRAY FOR ANALYZING GENE EXPRESSION OR GENOMIC VARIATIONS 审中-公开
    通用基因组分析基因表达或基因变异

    公开(公告)号:US20090061424A1

    公开(公告)日:2009-03-05

    申请号:US11847752

    申请日:2007-08-30

    Applicant: Fuqiang CHEN

    Inventor: Fuqiang CHEN

    CPC classification number: C12Q1/6809 C12Q2565/519 C12Q2561/125 C12Q2525/207

    Abstract: The present invention provides an array system comprising a plurality of immobilized oligonucleotides comprising artificial sequences and a plurality of complementary ligation templates, as well as methods and kits for using the array system to analyze populations of nucleic acids. In particular, target nucleic acids are ligated to the immobilized oligonucleotides on the array in the presence of the complementary ligation templates.

    Abstract translation: 本发明提供了一种阵列系统,其包括多个固定寡核苷酸,其包含人工序列和多个互补连接模板,以及使用阵列系统分析核酸群体的方法和试剂盒。 特别地,在互补连接模板存在下,将目标核酸连接到阵列上的固定的寡核苷酸。

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