公开(公告)号：US6013517A

公开(公告)日：2000-01-11

申请号：US850961

申请日：1997-05-05

Applicant: James G. Respess ,  Nicholas J. DePolo ,  Sunil Chada ,  Sybille Sauter ,  Mordechai Bodner ,  David A. Driver

Inventor： James G. Respess ,  Nicholas J. DePolo ,  Sunil Chada ,  Sybille Sauter ,  Mordechai Bodner ,  David A. Driver

IPC: C12N15/48 ,  C12N15/867 ,  C12N5/10 ,  C12N15/86

CPC classification number: C12N15/86 ,  C12N2740/13043 ,  C12N2740/13052

Abstract: Retroviral vector constructs are described which have a 5' LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3' LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression-cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gag/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.

Abstract translation: 描述了具有5'LTR，tRNA结合位点，包装信号，一个或多个异源序列，第二链合成起始和3'LTR的逆转录病毒载体构建体，其中该载体构建体缺乏逆转录病毒gag / pol或env 编码序列。 此外，描述了gag / pol和env表达盒，其中表达盒缺少多于8个核苷酸的连续序列。 上述逆转录病毒载体构建体，gag / pol和env表达盒可用于构建排除形成复制能力的病毒的生产细胞系。

公开(公告)号：US5789245A

公开(公告)日：1998-08-04

申请号：US741881

申请日：1996-10-30

Applicant: Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver

Inventor： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Carlos E. Ibanez ,  Stephen M. W. Chang ,  Douglas J. Jolly ,  David A. Driver

IPC: A61K38/00 ,  A61K48/00 ,  C07K14/02 ,  C07K14/045 ,  C07K14/075 ,  C07K14/155 ,  C07K14/16 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N5/16 ,  C12N9/24 ,  C12N15/00 ,  C12N15/113 ,  C12N15/33 ,  C12N15/63 ,  C12N15/86 ,  C12N15/861 ,  C12P21/02 ,  C12N15/40 ,  C12N5/10

CPC classification number: C12N15/113 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N15/1131 ,  C12N15/86 ,  C12N9/2402 ,  C12Y302/01045 ,  A61K2039/5256 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.

Abstract translation: 本发明提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法。

公开(公告)号：US20080226598A1

公开(公告)日：2008-09-18

申请号：US11712044

申请日：2007-02-27

Applicant: John Polo ,  Thomas W. Dubensky ,  Ilya Frolov ,  Jason P. Gardner ,  Gillis Otten ,  Susan Barnett ,  David A. Driver

Inventor： John Polo ,  Thomas W. Dubensky ,  Ilya Frolov ,  Jason P. Gardner ,  Gillis Otten ,  Susan Barnett ,  David A. Driver

IPC: A61K35/76 ,  C12N7/00 ,  C07H21/00 ,  A61P43/00 ,  C12N15/00 ,  C12N15/87

CPC classification number: C07K14/005 ,  A61K35/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2740/16122 ,  C12N2770/36143

Abstract: Methods are provided for generating immune responses utilizing alphavirus-based vector systems.

Abstract translation: 提供了使用基于甲病毒的载体系统产生免疫应答的方法。

公开(公告)号：US06342372B1

公开(公告)日：2002-01-29

申请号：US09350399

申请日：1999-07-08

Applicant: Thomas W. Dubensky, Jr. ,  John M. Polo ,  David A. Driver

Inventor： Thomas W. Dubensky, Jr. ,  John M. Polo ,  David A. Driver

IPC: C12N516

CPC classification number: C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N9/2402 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  C12Y302/01045 ,  Y02A50/388 ,  Y02A50/396 ,  Y02A50/466 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.

Abstract translation: 本发明提供了利用重组甲病毒载体的组合物和方法。 还公开了用于制备和利用真核分层载体引发系统的组合物和方法。

公开(公告)号：US6015686A

公开(公告)日：2000-01-18

申请号：US404796

申请日：1995-03-15

Applicant: Thomas W. Dubensky, Jr. ,  John M. Polo ,  Douglas J. Jolly ,  David A. Driver

Inventor： Thomas W. Dubensky, Jr. ,  John M. Polo ,  Douglas J. Jolly ,  David A. Driver

IPC: A61K38/00 ,  A61K48/00 ,  C07K14/02 ,  C07K14/045 ,  C07K14/075 ,  C07K14/155 ,  C07K14/16 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N5/16 ,  C12N9/24 ,  C12N15/00 ,  C12N15/113 ,  C12N15/33 ,  C12N15/63 ,  C12N15/86 ,  C12N15/861 ,  C12P21/02 ,  C12N15/11

CPC classification number: C12N15/113 ,  C07K14/005 ,  C07K14/47 ,  C07K14/705 ,  C07K14/755 ,  C07K14/82 ,  C12N15/1131 ,  C12N15/86 ,  C12N9/2402 ,  C12Y302/01045 ,  A61K2039/5256 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2710/10322 ,  C12N2710/16722 ,  C12N2730/10122 ,  C12N2740/15022 ,  C12N2740/16222 ,  C12N2770/36122 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85 ,  Y10S977/773 ,  Y10S977/804 ,  Y10S977/808 ,  Y10S977/918 ,  Y10S977/92 ,  Y10S977/924

Abstract: The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.