公开(公告)号：US08859726B2

公开(公告)日：2014-10-14

申请号：US13990135

申请日：2011-11-28

Applicant: Tomas Bjorkman ,  Gustav Rodrigo

Inventor： Tomas Bjorkman ,  Gustav Rodrigo

IPC: C07K1/22 ,  C07K1/16 ,  C07K16/00 ,  B01D15/38 ,  B01J20/32 ,  C07K16/06 ,  B01J20/286

CPC classification number: C07K1/22 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3208 ,  B01J20/3242 ,  B01J20/3274 ,  C07K16/065

Abstract: The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the C-terminal most proline residue after the third alpha-helix.

Abstract translation: 本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。 该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤吸附的含有蛋白质的免疫球蛋白的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。 该方法改进了以前的分离方法，因为每种配体包含蛋白A结构域（E，D，A，B，C）或蛋白质Z或其功能变体中的一种或多种，​​其中至少一种 在第三个α-螺旋之后具有C末端最多脯氨酸残基取代的单体。

公开(公告)号：US20130338339A1

公开(公告)日：2013-12-19

申请号：US13995977

申请日：2011-12-19

Applicant: Tomas Bjorkman ,  Bjorn Noren ,  Gustav Rodrigo ,  Jelena Vasic ,  Per-Mikael Aberg

Inventor： Tomas Bjorkman ,  Bjorn Noren ,  Gustav Rodrigo ,  Jelena Vasic ,  Per-Mikael Aberg

IPC: C07K16/12

CPC classification number: C07K16/1271 ,  B01D15/168 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/31

Abstract: The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine or Histidine at the position corresponding to H18 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.

Abstract translation: 本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。 该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤含有其上吸附有蛋白质的蛋白质的基质的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。 该方法改进了以前的分离方法，因为每种配体包含蛋白A结构域（E，D，A，B，C）或蛋白质Z或其功能变体中的一种或多种，​​其中至少一种 在相应于蛋白A或蛋白Z的B结构域的H18的位置处取代天门冬酰胺或组氨酸的单体，其中与未取代的配体相比，配体提供了洗脱pH的增加。

公开(公告)号：US20130081703A1

公开(公告)日：2013-04-04

申请号：US13704270

申请日：2011-05-05

Applicant: Catalin Andrei ,  Mikael Berg ,  Torbjorn Blank ,  Enrique Carredano ,  Karl Ekstrom ,  Tomas M. Karlsson ,  Jan-Erik Lynga ,  Roger Nordberg ,  Gustav Rodrigo ,  Henrik Sandegren

Inventor： Catalin Andrei ,  Mikael Berg ,  Torbjorn Blank ,  Enrique Carredano ,  Karl Ekstrom ,  Tomas M. Karlsson ,  Jan-Erik Lynga ,  Roger Nordberg ,  Gustav Rodrigo ,  Henrik Sandegren

IPC: F17D3/00

CPC classification number: F17D3/00 ,  G01N30/34 ,  G05D21/02 ,  Y10T137/0329

Abstract: A method of preparing a mixed liquid flow having predetermined characteristics, including a predetermined value of a first property and a predetermined value of a second property, comprising the steps of: a) providing a first set of at least one liquid flow each having a different first value of the first property; b) providing a second set of at least one liquid flow each having a different second value of the first property; c) providing a third set of at least one liquid flow of solvent; d) combining the provided liquid flows; and e) varying at least one of the liquid flows of the first and second sets and at least one liquid flow of the third set to adjust the first property and the second property to their respective predetermined values in the resulting mixed liquid flow.

Abstract translation: 一种制备具有预定特性的混合液流的方法，包括第一特性的预定值和第二特性的预定值，包括以下步骤：a）提供第一组至少一个液体流，每组具有不同的 第一财产的第一价值; b）提供第二组至少一个液体流，每个具有第一属性的不同的第二值; c）提供第三组至少一种液体的溶剂流; d）组合提供的液体流; 以及e）改变所述第一和第二组的液体流中的至少一个以及所述第三组的至少一个液体流，以将所述第一特性和所述第二特性调整到所得混合液体流中其各自的预定值。

公开(公告)号：US09187555B2

公开(公告)日：2015-11-17

申请号：US13995977

申请日：2011-12-19

Applicant: Tomas Bjorkman ,  Bjorn Noren ,  Gustav Rodrigo ,  Jelena Vasic ,  Per-Mikael Aberg

Inventor： Tomas Bjorkman ,  Bjorn Noren ,  Gustav Rodrigo ,  Jelena Vasic ,  Per-Mikael Aberg

IPC: C07K16/12 ,  C07K1/22 ,  C07K1/16 ,  B01D15/16 ,  B01D15/38 ,  B01J20/289 ,  C07K14/31 ,  B01J20/286 ,  B01J20/32

CPC classification number: C07K16/1271 ,  B01D15/168 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/31

Abstract: The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilized to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine or Histidine at the position corresponding to H18 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.

Abstract translation: 本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。 该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤含有其上吸附有蛋白质的蛋白质的基质的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。 该方法改进了以前的分离方法，因为每种配体包含蛋白A结构域（E，D，A，B，C）或蛋白质Z或其功能变体中的一种或多种，​​其中至少一种 在相应于蛋白A或蛋白Z的B结构域的H18的位置处取代天门冬酰胺或组氨酸的单体，其中与未取代的配体相比，配体提供了洗脱pH的增加。

公开(公告)号：US20120289680A1

公开(公告)日：2012-11-15

申请号：US13559663

申请日：2012-07-27

Applicant: MARTIN HALL ,  STURE LARSSON ,  ANDREAS MURANYI ,  GUSTAV RODRIGO ,  JINYU ZOU ,  PER-MIKAEL ÅBERG

Inventor： MARTIN HALL ,  STURE LARSSON ,  ANDREAS MURANYI ,  GUSTAV RODRIGO ,  JINYU ZOU ,  PER-MIKAEL ÅBERG

IPC: C07K14/31 ,  C07H21/04 ,  C07K1/22 ,  C07K16/00 ,  C07K17/00

CPC classification number: B01J20/24 ,  B01D15/3809 ,  B01J20/28019 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  B01J2220/54 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  C07K17/10 ,  C07K2317/55

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Abstract translation: 本发明涉及一种色谱配体，其包含来自葡萄球菌蛋白A（SpA）的结构域C，或其功能片段或变体。 色谱配体具有在现场（CIP）条件下耐受苛刻清洁的有利能力，并且能够结合抗体的Fab片段。 配体可以具有末端偶联基团，例如精氨酸或半胱氨酸，以促进其与不溶性载体例如珠粒或膜的偶联。 本发明还涉及在分离抗体中使用配体的方法以及可以包括洗涤步骤和/或用碱再生的纯化方案。

公开(公告)号：US20120217192A1

公开(公告)日：2012-08-30

申请号：US13497896

申请日：2010-09-23

Applicant: Torbjorn Blank ,  Enrique Carredano ,  Karl Ekstrom ,  Jan-Erik Lynga ,  Roger Nordberg ,  Mike Peters ,  Gustav Rodrigo ,  Henrik Sandegren ,  Lisa Stack

Inventor： Torbjorn Blank ,  Enrique Carredano ,  Karl Ekstrom ,  Jan-Erik Lynga ,  Roger Nordberg ,  Mike Peters ,  Gustav Rodrigo ,  Henrik Sandegren ,  Lisa Stack

IPC: G05D11/02 ,  B01D15/16 ,  G05D11/13

CPC classification number: B01D15/166 ,  B01F15/0022 ,  B01F15/00227 ,  G01N30/34 ,  G01N2030/347 ,  G05D11/132

Abstract: A method for preparing a liquid flow having pre-defined characteristics by mixing liquid flows of at least two different component stock solutions with each other comprises the steps of determining a selected property value for one or more of the stock solutions by sensing, in a flow of each stock solution separately, at least one characteristic related to the property value for the stock solution, and based on the determined property value or values, mixing the stock solution flows in mixing ratios giving the desired mixed liquid flow. A system for carrying out the method comprises sensor means and a control unit, wherein the control unit is arranged to evaluate the characteristics sensed by the sensor means and provide relative ratios of stock solutions required to obtain a mixed liquid flow having the pre-defined characteristics.

Abstract translation: 通过混合至少两种不同组分原料溶液的液体流相互混合制备具有预定特性的液体流的方法包括以下步骤：通过在流体中感测来确定一种或多种储备溶液的选定的特性值 分别存储与储备溶液的特性值相关的至少一个特征，并且基于所确定的特性值或值，以提供所需混合液体流的混合比混合储备液流。 用于执行该方法的系统包括传感器装置和控制单元，其中控制单元布置成评估由传感器装置感测到的特性，并提供获得具有预定特性的混合液流所需的储备溶液的相对比例 。