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11.发明授权
公开(公告)号：US5856108A
公开(公告)日：1999-01-05
申请号：US779694
申请日：1991-10-21
CPC分类号： C12N9/0036 , C12P17/12 , C12Q1/12 , G01N33/582
摘要： There is provided a method for producing diazoluminomelanin (DALM) which comprises culturing in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, a microorganism containing nitrate reductase.Also provided is a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube and activating luminescence.Further, there is provided a method for producing diazomelanin (DM) which comprises culturing in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, a microorganism containing nitrate reductase.Yet further, there is provided a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube, adding luminol and activating luminescence.
摘要翻译： 提供了一种生产重氮酚蓝蛋白（DALM）的方法，该方法包括在合适的代谢条件下，在含硝酸盐，3-氨基-L-酪氨酸（3-AT）和鲁米诺的培养基中培养含有硝酸还原酶的微生物。 还提供了一种用于直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐，3-氨基-L-酪氨酸（3-AT）和 将鲁米诺转移到适当的代谢条件下，将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管并激活发光。 此外，提供了一种生产重氮木兰素（DM）的方法，其包括在适当的代谢条件下在含有硝酸盐和3-氨基-L-酪氨酸（3-AT）的培养基中培养含有硝酸还原酶的微生物。 此外，提供了一种直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐和3-氨基-L-酪氨酸（3- AT），将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管，加入鲁米诺并激活发光。
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公开(公告)号：US4870002A
公开(公告)日：1989-09-26
申请号：US804819
申请日：1985-12-04
申请人： Johnathan L. Kiel
发明人： Johnathan L. Kiel
CPC分类号： C12N11/18 , A61K31/198
摘要： A process for preventing oxidative injury in which living cells are contacted with a protective amount of 3-amino-tyrosine or a derivative thereof. Also provided is a process for preserving a cell sample involving collecting a sample of cells and adding 3-aminotyrosine or a derivative thereof to that cell sample. A process for preventing thermal damage to living cells is also provided which comprises contacting the cells with a thermal protectant amount of 3-aminotyrosine or a derivative thereof, as is a process for protecting living cells from damage caused by ionizing radiation, which comprises contacting the cells with a radioprotectant amount of 3-aminotyrosine or a derivative thereof. Also provided is a process for suppressing anti-inflammatory reactions in animals which comprises administering an anti-inflammatory amount of 3-aminotrosine or a derivative thereof to an animal.
摘要翻译： 一种预防氧化损伤的方法，其中活细胞与保护量的3-氨基 - 酪氨酸或其衍生物接触。 还提供了一种保存细胞样品的方法，其涉及收集细胞样品并向该细胞样品中加入3-氨基酪氨酸或其衍生物。 还提供了防止活细胞热损伤的方法，其包括使细胞与热保护剂量的3-氨基酪氨酸或其衍生物接触，保护活细胞免受电离辐射引起的损伤的方法，该方法包括使 具有放射防护剂量的3-氨基酪氨酸或其衍生物的细胞。 还提供了抑制动物抗炎反应的方法，其包括向动物施用抗炎量的3-氨基酪氨酸或其衍生物。
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公开(公告)号：US4486408A
公开(公告)日：1984-12-04
申请号：US251694
申请日：1981-04-07
申请人： Johnathan L. Kiel
发明人： Johnathan L. Kiel
CPC分类号： A61K38/44 , A61K38/385 , A61K38/443 , A61K47/42 , A61K9/0019 , A61K9/14 , A61K9/146 , A61K9/19 , C12N11/18 , C12Y101/03004 , C12Y111/01007 , A61K9/06
摘要： A therapeutic preparation, and a process of using the preparation, for the control of tumor growth and immune diseases. The preparation is an immobilized cytotoxic enzyme system which is carried by an insoluble substrate that is administered into the mammalian subject by injection or implantation. The preferred embodiment of the preparation uses a polymerized albumin carrier containing immobilized glucose oxidase and peroxidase. The immobilized enzyme system is prepared in the form of small particles that can be administered either by implantation or by injection into tumor-bearing mammals. To date, antibacterial activity has been demonstrated only in vitro. Intraperitoneal injection into rats bearing Novikoff hepatomas or into mice bearing B16-F1 melanomas or L1210 (Lymphoid) tumors has resulted in the selected killing of tumor tissues. Several daily administrations are required to achieve complete destruction of the tumor. Additionally, the preparation is also active as a stimulant of the specific immune response to neoplasia. Its effect on the immune system could be useful in the stimulation of the immune system in diseases where the action of the endogenous immune system is insufficient.
摘要翻译： 治疗制剂和使用该制剂的方法用于控制肿瘤生长和免疫疾病。 该制剂是固定的细胞毒素酶系统，其由通过注射或植入施用于哺乳动物受试者的不溶性底物携带。 制剂的优选实施方案使用含有固定化葡萄糖氧化酶和过氧化物酶的聚合的白蛋白载体。 固定化酶系统以小颗粒的形式制备，可以通过植入或通过注射给含有哺乳动物的哺乳动物施用。 迄今为止，抗菌活性仅在体外证明。 腹腔注射带有Novikoff肝细胞的大鼠或携带B16-F1黑素瘤或L1210（淋巴样）肿瘤的小鼠导致选择性杀死肿瘤组织。 需要几个日常管理来实现肿瘤的完全破坏。 此外，该制剂也作为对瘤形成的特异性免疫应答的兴奋剂是有活性的。 其对免疫系统的影响可用于在内源性免疫系统的作用不足的疾病中刺激免疫系统。
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14.发明授权Compositions, methods and uses for biosynthetic plasmid integrated capture elements 有权生物合成质粒综合捕获元素的组成，方法和用途公开(公告)号：US08628955B2
公开(公告)日：2014-01-14
申请号：US12792492
申请日：2010-06-02
申请人： Johnathan L. Kiel , Amanda Tijerina , Eric A. Holwitt , Jill Parker , Mark A. Sloan , Melanie Woitaske , Maomian Fan
发明人： Johnathan L. Kiel , Amanda Tijerina , Eric A. Holwitt , Jill Parker , Mark A. Sloan , Melanie Woitaske , Maomian Fan
CPC分类号： B82Y5/00 , C12N15/111 , C12N2320/32 , C12N2330/00 , C12P19/34
摘要： Embodiments herein report compositions, systems and methods for making and using plasmid vectors and nanotube complexes. In certain embodiments, compositions, systems and methods herein include making plasmid vectors having aptamer inserts. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of plasmid vectors having aptamer inserts directed to a particular target agent. Other aspects concern plasmid constructs associated with organic semiconductors. Yet other aspects concern complexes of nanotubes associated with dsDNA aptamers and tracking molecules.
摘要翻译： 本文的实施方案报道了用于制备和使用质粒载体和纳米管复合物的组合物，系统和方法。 在某些实施方案中，本文的组合物，系统和方法包括制备具有适体插入片段的质粒载体。 在一些实施方案中，本文公开的方法可用于快速产生具有针对特定目标试剂的适配子插入物的大量质粒载体。 其他方面涉及与有机半导体相关的质粒构建体。 其他方面涉及与dsDNA适体和跟踪分子相关的纳米管的复合物。
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15.发明申请COMPOSITIONS, METHODS AND USES FOR BIOSYNTHETIC PLASMID INTEGRATED CAPTURE ELEMENTS 审中-公开用于生物化学PLASMID集成捕获元素的组合物，方法和用途公开(公告)号：US20130040305A1
公开(公告)日：2013-02-14
申请号：US13625770
申请日：2012-09-24
申请人： Johnathan L. KIEL , Amanda TIJERINA , Eric A. HOLWITT , Jill PARKER , Mark SLOAN , Melanie WOITASKE , Maomian FAN
发明人： Johnathan L. KIEL , Amanda TIJERINA , Eric A. HOLWITT , Jill PARKER , Mark SLOAN , Melanie WOITASKE , Maomian FAN
CPC分类号： B82Y5/00 , C12N15/111 , C12N2320/32 , C12N2330/00 , C12P19/34
摘要： Embodiments herein report compositions, systems and methods for making and using plasmid vectors and nanotube complexes. In certain embodiments, compositions, systems and methods herein include making plasmid vectors having aptamer inserts. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of plasmid vectors having aptamer inserts directed to a particular target agent. Other aspects concern plasmid constructs associated with organic semiconductors. Yet other aspects concern complexes of nanotubes associated with dsDNA aptamers and tracking molecules.
摘要翻译： 本文的实施方案报道了用于制备和使用质粒载体和纳米管复合物的组合物，系统和方法。 在某些实施方案中，本文的组合物，系统和方法包括制备具有适体插入片段的质粒载体。 在一些实施方案中，本文公开的方法可用于快速产生具有针对特定目标试剂的适配子插入物的大量质粒载体。 其他方面涉及与有机半导体相关的质粒构建体。 其他方面涉及与dsDNA适体和跟踪分子相关的纳米管的复合物。
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公开(公告)号：US20120134877A1
公开(公告)日：2012-05-31
申请号：US12973681
申请日：2010-12-20
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
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公开(公告)号：US20120021004A1
公开(公告)日：2012-01-26
申请号：US11820039
申请日：2007-05-25
CPC分类号： C12R1/07 , A61K39/07 , A61K2039/522
摘要： A new strain of Bacillus anthracis derived from the Sterne vaccine strain of Bacillus anthracis by growth on a high-nitrate-concentration, 3-amino-L-tyrosine growth medium.
摘要翻译： 通过在高硝酸盐浓度的3-氨基-L-酪氨酸生长培养基上生长，衍生自炭疽芽孢杆菌的Sterne疫苗株的新型芽孢杆菌菌株。
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18.发明申请METHODS AND COMPOSITIONS FOR PROCESSES OF RAPID SELECTION AND PRODUCTION OF NUCLEIC ACID APTAMERS 有权快速选择和生产核酸APTAMERS的方法和组合物公开(公告)号：US20090004644A1
公开(公告)日：2009-01-01
申请号：US11965039
申请日：2007-12-27
IPC分类号： C12Q1/70 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6811 , C12N15/115 , Y10T436/143333 , C12Q2525/205 , C12Q2565/101
摘要： Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
摘要翻译： 本文的实施方案涉及用于制备和使用适体的组合物和方法，例如DNA适体（DCE）和/或RNA适体。 在一些实施方案中，方法涉及制备和扩增目标DCE。 在某些实施方案中，用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。 在一些实施方案中，本文公开的方法可用于快速产生大量适配体，例如针对特定目标试剂的DCE。 一些实施例涉及用于执行适配体的自动生成的系统。
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公开(公告)号：US06569630B1
公开(公告)日：2003-05-27
申请号：US09978753
申请日：2001-10-15
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods of preparing nucleic acid ligands against anthrax spores, compositions comprising anthrax specific nucleic acid ligands and methods of use of such ligands for detection and/or neutralization of anthrax spores.
摘要翻译： 本发明涉及制备针对炭疽孢子的核酸配体的方法，包含炭疽特异性核酸配体的组合物和用于检测和/或中和炭疽孢子的这种配体的使用方法。
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20.发明授权Nitrate reductase-transfected HeLa cells for cancer and microwave bioeffects research 失效硝酸还原酶转染的HeLa细胞用于癌症和微生物的生物效应研究公开(公告)号：US06326196B1
公开(公告)日：2001-12-04
申请号：US09766017
申请日：2001-01-22
申请人： Jill E. Parker , Johnathan L. Kiel
发明人： Jill E. Parker , Johnathan L. Kiel
IPC分类号： C12N508
CPC分类号： G01N33/5011 , G01N33/5008 , G01N33/502
摘要： The cell line HeLa is transformed with the chromosomal insertion of the plasmid pSV2neoNR101, ATCC No. 69617. The transformed cells, HeLaNR1, produce diazoluminomelanin (DALM) intra cellularly when provided with nitrate, luminol and 3-amino-L-tyrosine•HC1 (3AT). The modified cells can be used to study mechanisms for radiofrequency and light radiation interactions with carcinoma of the cervix. The effects of drugs, hormones, and cytokines that affect the expression of nitric oxide synthase and its activity can also be studied to understand the effects of these materials on cervix cells.
摘要翻译： 细胞系HeLa用质粒pSV2neoNR101，ATCC No.69617的染色体插入转化。当提供硝酸盐，鲁米诺和3-氨基-L-酪氨酸时，转化细胞HeLaNR1在细胞内产生二氮嘧啶蛋白（DALM） 3AT）。 修饰的细胞可用于研究与子宫颈癌的射频和光辐射相互作用的机制。 还可以研究影响一氧化氮合酶表达的药物，激素和细胞因子的作用及其活性，以了解这些材料对子宫颈细胞的影响。
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