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公开(公告)号：US08383036B2
公开(公告)日：2013-02-26
申请号：US12973681
申请日：2010-12-20
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
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2.发明授权公开(公告)号：US07892484B2
公开(公告)日：2011-02-22
申请号：US10291336
申请日：2002-11-08
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
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公开(公告)号：US06878519B1
公开(公告)日：2005-04-12
申请号：US10339259
申请日：2003-01-06
申请人： Jill E. Parker , Johnathan L. Kiel
发明人： Jill E. Parker , Johnathan L. Kiel
摘要： A PCR-based method for the identification of Bacillus anthracis is described. The method utilizes novel primer sets; designated 2Xlg3F (SEQ ID NO 3), 2Xlg3R (SEQ ID NO 4), 2Xlg3F2 (SEQ ID NO 5), 2Xlg3R2 (SEQ ID NO 6), 4XH1a2F (SEQ ID NO 7), and 4XH1a2R (SEQ ID NO 8).
摘要翻译： 描述了用于鉴定炭疽芽孢杆菌的基于PCR的方法。 该方法利用新型引物组; 命名为2X1g3F（SEQ ID NO3），2X1G3R（SEQ ID NO4），2X1g3F2（SEQ ID NO：5），2X1g3R2（SEQ ID NO：6），4XH1a2F（SEQ ID NO：7）和4XH1a2R（SEQ ID NO：8）。
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公开(公告)号：US5972721A
公开(公告)日：1999-10-26
申请号：US816429
申请日：1997-03-14
申请人： John G. Bruno , Johnathan L. Kiel , John P. Kilian
发明人： John G. Bruno , Johnathan L. Kiel , John P. Kilian
CPC分类号： B03C1/032 , B03C1/01 , B03C1/0332 , B03C1/034 , Y10S436/824
摘要： An apparatus and method for immunomagnetic separation and concentration of target biological materials is disclosed. The immunomagnetic separation is performed by a magnetic flow cell, or filter block, as part of an automated mostly continuous immunomagnetic assay system. The magnetic flow cell has two bundles of ferromagnetic rods or pins positioned inside an internal chamber so that a fluid sample flowing through the flow cell passes through the pins. A pair of cobalt magnets flank the flow cell so that the pins concentrate and sufficiently increase the magnetic fields so that even nanometer size magnetic beads can be captured. The overall system combines a reaction subsystem for reacting coated magnetic beads with a sample, a collection subsystem for capturing magnetic beads, a rinsing subsystem for removing debris and a filtering subsystem for removing captured magnetic beads from the collection subsystem. The new magnetic flow filter is the key component for the collection and filtering subsystems.
摘要翻译： 公开了一种用于免疫磁性分离和靶向生物材料浓缩的装置和方法。 免疫磁性分离由磁流量池或过滤块进行，作为自动化大多数连续的免疫磁性测定系统的一部分。 磁流量计具有两束位于内部室内的铁磁棒或销，使得流过流动池的流体样品通过销。 在流动池侧面的一对钴磁体，使得引脚集中并充分增加磁场，使得可以捕获甚至纳米尺寸的磁珠。 整个系统结合了用于使涂覆的磁珠与样品反应的反应子系统，用于捕获磁珠的收集子系统，用于去除碎屑的冲洗子系统和用于从收集子系统中去除捕获的磁珠的过滤子系统。 新的磁流过滤器是收集和过滤子系统的关键组件。
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公开(公告)号：US5801054A
公开(公告)日：1998-09-01
申请号：US716691
申请日：1996-09-19
申请人： Johnathan L. Kiel , John L. Alls
发明人： Johnathan L. Kiel , John L. Alls
摘要： A cell culture device is described comprising a gas permeable vessel which contains one or more self contained carbon dioxide generators and which is attached to a closure closing a gas permeable cell culture flask. Located in the closure is a gas permeable insert which defines a gas permeable opening through which the gases from the atmosphere of the vessel produced by the carbon dioxide generators can communicate with the gases in the atmosphere of the flask. This invention allows the rapid and uniform equilibration of the atmosphere of the flask with the controlled atmosphere of the vessel while still providing a closed system which prevents the entry of microbial organisms into the flask. This invention provides a controlled atmosphere for up to 24 hours that maintains the pH of the culture medium for optimal growth of cells growing in the culture medium in the flask. This invention allows for continuous microscopic observation of cells during this period and for long term exposure of cells to various physical or chemical agents.
摘要翻译： 描述了一种细胞培养装置，其包括含有一个或多个自含二氧化碳发生器的气体可渗透容器，并且附着于闭合气体可渗透细胞培养瓶的封闭件。 位于封闭件中的是可透气的插入件，其限定了可透气的开口，来自二氧化碳发生器产生的容器的气体的气体可以通过该气体与烧瓶大气中的气体连通。 本发明允许烧瓶的气氛与容器的受控气氛快速均匀地平衡，同时仍然提供一种封闭系统，其防止微生物进入烧瓶。 本发明提供可控制的气氛长达24小时，其维持培养基的pH以使培养基中培养基中生长的细胞生长最佳。 本发明允许在此期间连续显微镜观察细胞，并允许细胞长期暴露于各种物理或化学试剂。
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公开(公告)号：US5089385A
公开(公告)日：1992-02-18
申请号：US521064
申请日：1990-05-07
IPC分类号： C12M3/00
CPC分类号： C12M41/12 , C12M29/10 , C12M29/26 , Y10S435/813
摘要： A flow-through cell cultivation apparatus and method is described. The flow-through cell cultivation system comprises a flowcell, a chamber inside the flowcell for holding cells, and a flowcell holder. The flowcell has a lower intake port for flowing liquid nutrient through a cellbed inside the flowcell and an upper outlet port for flowing the liquid nutrient out of the flowcell. The flowcell also includes a chuck for receipt of a thermal probe. The probe is made of electromagnetically non-interactive material. The flowcell is enclosed inside the flowcell holder. The flowcell holder includes a pair of intake ports into a cavity having an open end at the flowcell for turbulently flowing a temperature controlled gas against the flowcell. The cavity has rough walls to promote the turbulent flow. The flowcell holder includes an exhaust port for flowing the gas out of the flowcell holder which also serves as a port for another thermal probe. A one-way valve prevents flow of the liquid nutrient out of the chamber through the intake port. Temperature controlled air is supplied to the flowcell holder by dividing air from an air supply into two conduits. One conduit travels through a coil inside a refrigerated liquid bath and the other through a coil inside a heated liquid bath. The heated and cooled air are recombined to make an even temperature air supply which is then divided once more to provide the two air supplies for creating a turbulent supply of temperature controlled air through the two flowcell holder intake ports. The flowcell may include on one side a semi-permeable membrane for introducing a gas/liquid interface to the cellbed.
摘要翻译： 描述了流通细胞培养装置和方法。 流通池培养系统包括流通池，用于保持电池的流通池内的室和流通池保持器。 流通池具有用于使液体营养物流过流动池内的池底的较低进气口和用于使液体营养物流出流动池的上部出口。 流通池还包括用于接收热探针的卡盘。 探头由电磁非交互材料制成。 流通池封闭在流通池支架内。 流通池保持器包括一对进入端口，其具有在流通池处具有开口端的空腔，用于将受温度控制的气体湍流地流向流动池。 空腔具有粗糙的壁，以促进湍流。 流通池保持器包括用于将气体从流动池保持器流出的排气口，其还用作另一热探针的端口。 单向阀防止液体营养物质通过进气口流出室外。 通过将空气从空气供应分成两个管道，将温度控制的空气供应到流通池保持器。 一个导管穿过冷藏液槽内的线圈，另一个通过加热液槽内的线圈。 加热和冷却的空气被重新组合以产生均匀的温度空气供应，然后再分配一次，以提供两个空气供应，以通过两个流通池保持器进气口产生温度受控空气的湍流供应。 流动池可以在一侧包括用于将气体/液体界面引入细胞床的半透膜。
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公开(公告)号：US5028541A
公开(公告)日：1991-07-02
申请号：US56034
申请日：1987-06-01
IPC分类号： C12M3/00
摘要： A flow-through cell cultivation apparatus and method is described. The flow-through cell cultivation system comprises a flowcell, a chamber inside the flowcell for holding cells, and a flowcell holder. The flowcell has a lower intake port for flowing liquid nutrient through a cellbed inside the flowcell and an upper outlet port for flowing the liquid nutrient out of the flowcell. The flowcell also includes a chuck for receipt of a thermal probe. The probe is made of electromagnetically non-interactive material. The flowcell is enclosed inside the flowcell holder. The flowcell holder includes a pair of intake ports into a cavity having an open end at the flowcell for turbulently flowing a temperature controlled gas against the flowcell. The cavity has rough walls to promote the turbulent flow. The flowcell holder includes an exhaust port for flowing the gas out of the flowcell holder which also serves as a port for another thermal probe. A one-way valve prevents flow of the liquid nutrient out of the chamber through the intake port. Temperature controlled air is supplied to the flowcell holder by dividing air from an air supply into two conduits. One conduit travels through a coil inside a refrigerated liquid bath and the other through a coil inside a heated liquid bath. The heated and cooled air are recombined to make an even temperature air supply which is then divided once more to provide the two air supplies for creating a turbulent supply of temperature controlled air through the two flowcell holder intake ports. The flowcell may include on one side a semi-permeable membrane for introducing a gas/liquid interface to the cellbed.
摘要翻译： 描述了流通细胞培养装置和方法。 流通池培养系统包括流通池，用于保持电池的流通池内的室和流通池保持器。 流通池具有用于使液体营养物流过流动池内的池底的较低进气口和用于使液体营养物流出流动池的上部出口。 流通池还包括用于接收热探针的卡盘。 探头由电磁非交互材料制成。 流通池封闭在流通池支架内。 流通池保持器包括一对进入端口，其具有在流通池处具有开口端的空腔，用于将受温度控制的气体湍流地流向流动池。 空腔具有粗糙的壁，以促进湍流。 流通池保持器包括用于将气体从流动池保持器流出的排气口，其还用作另一热探针的端口。 单向阀防止液体营养物质通过进气口流出室外。 通过将空气从空气供应分成两个管道，将温度控制的空气供应到流通池保持器。 一个导管穿过冷藏液槽内的线圈，另一个通过加热液槽内的线圈。 加热和冷却的空气被重新组合以产生均匀的温度空气供应，然后再分配一次，以提供两个空气供应，以通过两个流通池保持器进气口产生温度受控空气的湍流供应。 流动池可以在一侧包括用于将气体/液体界面引入细胞床的半透膜。
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8.发明授权公开(公告)号：US09273345B2
公开(公告)日：2016-03-01
申请号：US11965039
申请日：2007-12-27
IPC分类号： C12N15/115 , C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/115 , Y10T436/143333 , C12Q2525/205 , C12Q2565/101
摘要： Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
摘要翻译： 本文的实施方案涉及用于制备和使用适体的组合物和方法，例如DNA适体（DCE）和/或RNA适体。 在一些实施方案中，方法涉及制备和扩增目标DCE。 在某些实施方案中，用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。 在一些实施方案中，本文公开的方法可用于快速产生大量适配体，例如针对特定目标试剂的DCE。 一些实施例涉及用于执行适配体的自动生成的系统。
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公开(公告)号：US08092988B1
公开(公告)日：2012-01-10
申请号：US11820039
申请日：2007-05-25
申请人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls , Pedro J. Morales, legal representative
发明人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls
CPC分类号： C12R1/07 , A61K39/07 , A61K2039/522
摘要： A new strain of Bacillus anthracis derived from the Sterne vaccine strain of Bacillus anthracis by growth on a high-nitrate-concentration, 3-amino-L-tyrosine growth medium.
摘要翻译： 通过在高硝酸盐浓度的3-氨基-L-酪氨酸生长培养基上生长，衍生自炭疽芽孢杆菌的Sterne疫苗株的新型芽孢杆菌菌株。
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10.发明申请COMPOSITIONS, METHODS AND USES FOR BIOSYNTHETIC PLASMID INTEGRATED CAPTURE ELEMENTS 有权用于生物化学PLASMID集成捕获元素的组合物，方法和用途公开(公告)号：US20110027851A1
公开(公告)日：2011-02-03
申请号：US12792492
申请日：2010-06-02
申请人： Johnathan L. KIEL , Amanda TIJERINA , Eric A. HOLWITT , Jill PARKER , Mark A. SLOAN , Melanie WOITASKE , Maomian FAN
发明人： Johnathan L. KIEL , Amanda TIJERINA , Eric A. HOLWITT , Jill PARKER , Mark A. SLOAN , Melanie WOITASKE , Maomian FAN
CPC分类号： B82Y5/00 , C12N15/111 , C12N2320/32 , C12N2330/00 , C12P19/34
摘要： Embodiments herein report compositions, systems and methods for making and using plasmid vectors and nanotube complexes. In certain embodiments, compositions, systems and methods herein include making plasmid vectors having aptamer inserts. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of plasmid vectors having aptamer inserts directed to a particular target agent. Other aspects concern plasmid constructs associated with organic semiconductors. Yet other aspects concern complexes of nanotubes associated with dsDNA aptamers and tracking molecules.
摘要翻译： 本文的实施方案报道了用于制备和使用质粒载体和纳米管复合物的组合物，系统和方法。 在某些实施方案中，本文的组合物，系统和方法包括制备具有适体插入片段的质粒载体。 在一些实施方案中，本文公开的方法可用于快速产生具有针对特定目标试剂的适配子插入物的大量质粒载体。 其他方面涉及与有机半导体相关的质粒构建体。 其他方面涉及与dsDNA适体和跟踪分子相关的纳米管的复合物。
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