公开(公告)号：US08357376B2

公开(公告)日：2013-01-22

申请号：US12652557

申请日：2010-01-05

Applicant: Jonathan Liu ,  Richard Schwartz ,  Mark Thompson ,  Luis Jorge Camilo Maranga ,  Simon Sheng-Tsiung Hsu ,  Mridul Ghosh ,  Ajit Subramanian

Inventor： Jonathan Liu ,  Richard Schwartz ,  Mark Thompson ,  Luis Jorge Camilo Maranga ,  Simon Sheng-Tsiung Hsu ,  Mridul Ghosh ,  Ajit Subramanian

IPC: A61K39/145

CPC classification number: C12N7/00 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16251 ,  C12N2760/16252

Abstract: The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.

Abstract translation: 本发明涉及可以在细胞培养中将病毒（例如流感病毒）生长至比先前可能更高滴度的新型MDCK细胞。 MDCK细胞可适应于无血清培养基。 本发明还涉及包含MDCK细胞的细胞培养组合物和用于培养MDCK细胞的培养方法。 本发明还涉及使用本发明的MDCK细胞在细胞培养中生产流感病毒的方法。

公开(公告)号：US20120258136A1

公开(公告)日：2012-10-11

申请号：US13473494

申请日：2012-05-16

Applicant: Jonathan LIU ,  Mark Thompson ,  Luis J. Maranga ,  Floro Cataniag ,  Simon S. Hsu

Inventor： Jonathan LIU ,  Mark Thompson ,  Luis J. Maranga ,  Floro Cataniag ,  Simon S. Hsu

IPC: A61K39/145 ,  A61P37/04 ,  A61P31/16 ,  C12N7/02

CPC classification number: C12N7/02 ,  A61K39/12 ,  A61K39/145 ,  C12N7/00 ,  C12N2760/16051 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251

Abstract: The present invention provides novel serum-free cell culture medium and methods for cultivating MDCK cells. In particular, non-tumorigenic MDCK cells. The present invention also provides methods for producing influenza viruses (e.g., particularly cold-adapted, and/or temperature sensitive, and/or attenuated influenza viruses) that eliminate the need for a cell culture medium exchange step. The novel medium and methods are useful to grow influenza viruses, in cell culture to high titer. The present invention further provides purification methods for purifying influenza viruses with high overall recovery of live virus and result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.

Abstract translation: 本发明提供新型的无血清细胞培养基和培养MDCK细胞的方法。 特别是非致瘤性MDCK细胞。 本发明还提供了消除对细胞培养基交换步骤的需要的用于生产流感病毒（例如，特别冷适应的和/或温度敏感的和/或减毒的流感病毒）的方法。 新型培养基和方法可用于在细胞培养物中高效滴加流感病毒。 本发明还提供了用于纯化具有高活性病毒总体回收率的流感病毒的纯化方法，并导致宿主细胞DNA（HCD），宿主细胞蛋白（HCP）和非特异性内切核酸酶（例如，Benzonase）的水平，其低于 监管机构要求的规格。 免疫原性组合物可用于主动免疫受试者或产生用于多种用途的抗体，包括被动免疫和诊断性免疫测定。

公开(公告)号：US07675818B2

公开(公告)日：2010-03-09

申请号：US11989211

申请日：2006-07-05

Applicant: Jonathan Liu ,  Lorie K. Bear ,  Jerome R. Krebs

Inventor： Jonathan Liu ,  Lorie K. Bear ,  Jerome R. Krebs

IPC: G01V1/28

CPC classification number: G01V1/303

Abstract: Method for reducing instability and increasing computational efficiency in tomographic inversion for velocity model building. A system of tomographic equations is developed for a uniform grid. A non-uniform parameterization is found for which a linear mapping exists between the space of the uniform grid and the space of the non-uniform grid. The matrix that relates velocity to the tomographic data in the non-uniform representation is then given by the matrix product of the corresponding matrix in the uniform grid representation and the mapping matrix. Inversion can then be performed for the non-uniform parameterization on a smaller, more stable matrix.

Abstract translation: 降低速度模型建立断层反演中不稳定性和提高计算效率的方法。 开发了一种用于统一网格的断层方程系统。 在均匀网格的空间与非均匀网格的空间之间存在线性映射，发现了非均匀参数化。 然后在均匀网格表示和映射矩阵中，通过相应矩阵的矩阵乘积给出将速度与非均匀表示中的断层数据相关联的矩阵。 然后可以对较小，更稳定的矩阵进行非均匀参数化的反转。

公开(公告)号：US20090120634A1

公开(公告)日：2009-05-14

申请号：US11989211

申请日：2006-07-05

Applicant: Jonathan Liu ,  Lorie K. Bear ,  Jerome R. Krebs

Inventor： Jonathan Liu ,  Lorie K. Bear ,  Jerome R. Krebs

IPC: G01V1/28 ,  E21B43/00 ,  E21B47/00 ,  G06F17/10

CPC classification number: G01V1/303

Abstract: Method for reducing instability and increasing computational efficiency in tomographic inversion for velocity model building. A system of tomographic equations is developed for a uniform grid. A non-uniform parameterization is found for which a linear mapping exists between the space of the uniform grid and the space of the non-uniform grid. The matrix that relates velocity to the tomographic data in the non-uniform representation is then given by the matrix product of the corresponding matrix in the uniform grid representation and the mapping matrix. Inversion can then be performed for the non-uniform parameterization on a smaller, more stable matrix.

Abstract translation: 降低速度模型建立断层反演中不稳定性和提高计算效率的方法。 开发了一种用于统一网格的断层方程系统。 在均匀网格的空间与非均匀网格的空间之间存在线性映射，发现了非均匀参数化。 然后在均匀网格表示和映射矩阵中，通过相应矩阵的矩阵乘积给出将速度与非均匀表示中的断层数据相关联的矩阵。 然后可以对较小，更稳定的矩阵进行非均匀参数化的反转。

公开(公告)号：US20070075792A1

公开(公告)日：2007-04-05

申请号：US11241594

申请日：2005-09-30

Applicant: Jonathan Liu

Inventor： Jonathan Liu

IPC: H03K3/03 ,  G01N37/00

CPC classification number: G01R31/2882 ,  G01R31/2856 ,  H03K3/0315 ,  H03K5/159

Abstract: Provided are a method, system, and device to monitor degradation of a signal due to circuit aging. In one embodiment, a signal may be applied to a data path prior to aging of the circuit producing the signal to provide a reference value. The signal generating circuit may then be aged while the data path is disabled to protect the data path from the effects of circuit aging. Upon reenabling the data path, the signal may be reapplied in an after stress test to measure the effects of circuit aging on the circuitry generating the signal. For example, the effects of circuit aging may be measured for clock duty cycle degradation, clock skew degradation and signal margin degradation as well as other signal parameters. Additional embodiments are described and claimed.

Abstract translation: 提供了一种用于监测由于电路老化引起的信号劣化的方法，系统和装置。 在一个实施例中，可以在产生信号的电路老化之前将信号施加到数据路径以提供参考值。 然后，当数据路径被禁用时，信号发生电路可以被老化，以保护数据路径免受电路老化的影响。 在重新启用数据路径之后，信号可以在后应力测试中重新应用，以测量电路老化对产生信号的电路的影响。 例如，可以测量电路老化的影响，用于时钟占空比退化，时钟偏差劣化和信号余量劣化以及其他信号参数。 描述和要求保护附加的实施例。

公开(公告)号：US20050134394A1

公开(公告)日：2005-06-23

申请号：US10745427

申请日：2003-12-23

Applicant: Jonathan Liu

Inventor： Jonathan Liu

IPC: G01R31/317 ,  H03B1/00

CPC classification number: G01R31/31703 ,  G01R31/31727

Abstract: Embodiments of the invention include on-chip characterization of transistor degradation. In one embodiment, includes one or more functional blocks to perform one or more functions and an integrated on-chip characterization circuit to perform on-chip characterization of transistor degradation. The integrated on-chip characterization circuit includes a selectively enabled ring oscillator to generate a reference oscillating signal, a free-running ring oscillator to generate a free-running oscillating signal, and a comparison circuit coupled to the selectively enabled ring oscillator and the free-running ring oscillator. From the reference oscillating signal and the free-running oscillating signal, the comparison circuit determine a measure of transistor degradation.

Abstract translation: 本发明的实施例包括晶体管劣化的芯片表征。 在一个实施例中，包括执行一个或多个功能的一个或多个功能块和用于执行晶体管劣化的芯片表征的集成片上表征电路。 集成片上表征电路包括有选择地使能的环形振荡器以产生参考振荡信号，自由运行的环形振荡器以产生自由运行的振荡信号，以及比较电路，耦合到有选择地使能的环形振荡器和自由振荡环形振荡器， 运行环形振荡器。 从参考振荡信号和自由运行的振荡信号，比较电路确定晶体管退化的测量。

公开(公告)号：US06539485B1

公开(公告)日：2003-03-25

申请号：US09347729

申请日：1999-07-06

Applicant: Jonathan Liu ,  Christine Liu

Inventor： Jonathan Liu ,  Christine Liu

IPC: G06F132

CPC classification number: G06F1/3203

Abstract: Intelligence is added to a computer sleep mode indicator system. A method for an intelligent indicator system is provided to turn off the sleep mode status indicator of a computer during sleep mode when a user is known to be unavailable to monitor the indicator, for example, when the user is sleeping or having lunch. This embodiment also handles reasonable variations of user habits. Further, to account for a gradual change of user unavailability habits over time, this invention obtains and applies a user unavailability intelligence by dynamically modifying the beginning and the ending times of a specified user unavailability period based on user experience. One preferred method for dynamically modifying the beginning and ending times is by calculating the moving averages of the actual sleep mode entry and exit times that occurred within a pre-determined period from the specified beginning and ending times.

Abstract translation: 智能被添加到计算机睡眠模式指示器系统中。 提供了一种用于智能指示器系统的方法，当用户已知不能监视指示符（例如，当用户正在睡觉或吃午饭时）时，在睡眠模式期间关闭计算机的睡眠模式状态指示器。 该实施例还处理用户习惯的合理变化。 此外，为了解决用户不可用性随时间的变化，本发明通过基于用户体验动态修改指定的用户不可用期间的开始和结束时间来获得并应用用户不可用性智能。 用于动态修改开始和结束时间的一种优选方法是通过从指定的开始和结束时间计算在预定时段内发生的实际睡眠模式进入和退出时间的移动平均值。

公开(公告)号：US5623634A

公开(公告)日：1997-04-22

申请号：US448003

申请日：1995-05-23

Applicant: Jonathan Liu

Inventor： Jonathan Liu

IPC: G06F13/18 ,  G06F13/376

CPC classification number: G06F13/18

Abstract: A controller for a single port (typically FIFO) memory for a computer system allocates the flow of data from a single port dynamic RAM to several data requesters such as a graphics engine, a CPU, and a display screen. One parameter is assigned to the display screen and a second parameter is assigned to the other data requester. Then each memory cycle is assigned in duty cycle fashion to the data requesters associated with one or the other of the parameters. Thus, typically the screen display which requires large amounts of data will have a relatively large parameter value associated with it such as six, while the single parameter value associated with the graphic engine and CPU will have a lower value such as two. The screen display will in response access the memory for six memory cycles consecutively, while the graphics engine and CPU will access the memory for only two memory cycles. The FIFO associated with the screen display is thus kept relatively full so that the screen is refreshed at all times and no FIFO empty detection is thus needed. The memory controller includes a loadable counter for counting down the loaded parameter, and a state machine for loading in the values, decrementing the values held in the counter, and switching between the two parameters.

Abstract translation: 用于计算机系统的单个端口（通常为FIFO）存储器的控制器将数据流从单个端口动态RAM分配给若干数据请求者，例如图形引擎，CPU和显示屏幕。 一个参数被分配给显示屏幕，第二个参数被分配给另一个数据请求者。 然后每个存储周期以占空比方式分配给与一个或另一个参数相关联的数据请求者。 因此，通常需要大量数据的屏幕显示将具有与其相关联的诸如6的相对大的参数值，而与图形引擎和CPU相关联的单个参数值将具有较低的值，例如两个。 屏幕显示将响应连续访问存储器六个存储器周期，而图形引擎和CPU将只访问存储器只有两个存储器周期。 因此，与屏幕显示相关联的FIFO保持相对完整，使得屏幕始终刷新，因此不需要FIFO空的检测。 存储器控制器包括用于对加载的参数进行计数的可加载计数器，以及用于加载值的状态机，递减保持在计数器中的值以及在两个参数之间切换。

公开(公告)号：US20160109589A1

公开(公告)日：2016-04-21

申请号：US14818958

申请日：2015-08-05

Applicant: Jonathan Liu

Inventor： Jonathan Liu

IPC: G01V1/28

CPC classification number: G01V1/282 ,  G01V1/301 ,  G01V1/303 ,  G01V1/306 ,  G01V2210/51 ,  G01V2210/52 ,  G01V2210/614 ,  G01V2210/622 ,  G01V2210/626 ,  G01V2210/671 ,  G01V2210/675

Abstract: Method for building a subsurface model of velocity or other elastic property from seismic reflection data using tomography. The method uses velocity scans to pick a focusing velocity model at each image point (40). The focusing velocities are used to pick depth errors from tables (60) generated using a tomographic inversion matrix (30) and a suite of different velocity models (10). The depth errors are then reconstructed at each image point from the velocity scans based on the difference between the base velocity model and the most coherent velocity from the scan (70). The reconstructed depth errors are used to compute the velocity model update (80).

Abstract translation: 使用断层扫描从地震反射数据建立速度或其他弹性特性地下模型的方法。 该方法使用速度扫描在每个图像点选择聚焦速度模型（40）。 聚焦速度用于从使用断层反演矩阵（30）和一组不同速度模型（10）生成的表（60）中挑取深度误差。 然后根据基本速度模型与扫描中最相干速度的差异，从速度扫描的每个图像点重建深度误差（70）。 重建深度误差用于计算速度模型更新（80）。

公开(公告)号：US08271206B2

公开(公告)日：2012-09-18

申请号：US12427409

申请日：2009-04-21

Applicant: Changsheng Jonathan Liu ,  Yiqiong Wu ,  Kevin Jay LeVan

Inventor： Changsheng Jonathan Liu ,  Yiqiong Wu ,  Kevin Jay LeVan

IPC: G06F19/00 ,  G06F15/00

CPC classification number: G06F19/22

Abstract: Certain embodiments of the invention provide systems and methods for the automated assembly of DNA sequence data into contiguous DNA segments using a computer a system. DNA sequence data is entered into the system. The system indexes and groups a plurality of DNA fragment reads utilizing an anchor sequence and consolidates the fragments into larger sequences by merging the fragment reads within a group.

Abstract translation: 本发明的某些实施方案提供了使用计算机系统将DNA序列数据自动装配到连续DNA片段中的系统和方法。 DNA序列数据输入到系统中。 该系统使用锚定序列指数和组合多个DNA片段读取，并通过合并一组中的片段读取将片段合并成更大的序列。