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公开(公告)号：US06448008B1
公开(公告)日：2002-09-10
申请号：US09609035
申请日：2000-06-30
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07H19/04 , C09B23/02 , G01N33/533 , G01N33/582
摘要： A fluorescent cyanine dye of the following general formula is disclosed: wherein: X1 and X2 are independently selected from the group consisting of —O—, —S—, —C(CH3)2 or —C═CH2; Y1 and Y2 are nonmetal atoms required to form a benzo-condensed or naphtho-condensed ring; Q is a conjugated moiety that increases the fluorescent quantum yield and the stability of the compound; R1 and R2 are independently selected from the group consisting of H, C1-C4, alkyl, alkylensulfonic group or alkylensulfonate group wherein the alkylene group has from 1 to 4 carbon atoms; R3, R4 and R5 are independently selected from the group consisting of H, a sulfonic group, a sulfonate group, alkylensulfonic, alkylensulfonate and —SO2NH(CH2)m—W—(CH2)nZ, wherein alkylene has 1 to 4 carbon atoms, with the proviso that at least one of R1 to R5 contains a sulfonic or sulfonate group; W is absent or is a group selected from —SO2NH, —O—, —COO—, or —CONH—; n=0-12 and m=0-12 with the provisos that m+n≦12 and at least one of m and n≠0; and Z is, or contains a N, O or S nucleophile functionality or is, or contains a functionality capable of reacting with N, O or S nucleophiles. Nucleophile functionalities include —NH2, —OH, and —SH groups; groups capable of reacting with such functionalities include —COCl, —COOCOR, —CONHNH2, N-hydroxysuccinimido esters, —NCS, —CHO, —COCH2I, phosphoramidite and maleimido.
摘要翻译： 公开了以下通式的荧光花青染料：其中：X 1和X 2独立地选自-O - ， - S - ， - C（CH 3）2或-C = CH 2; Y 1和Y 2是非金属 需要形成苯并稠合或萘并稠环的原子; Q是增加荧光量子产率和化合物稳定性的共轭部分; R1和R2独立地选自H，C1-C4，烷基，亚烷基磺基或亚烷基磺酸酯基，其中亚烷基具有1〜 4个碳原子; R 3，R 4和R 5独立地选自H，磺酸基，磺酸基，亚烷基磺酸，亚烷基磺酸酯和-SO 2 NH（CH 2）m W-（CH 2）n Z，其中亚烷基具有1至4个碳原子， 条件是R 1至R 5中的至少一个含有磺酸基或磺酸酯基; W不存在或是选自-SO 2 NH，-O - ， - COO-或-CONH-的基团; n = 0-12且m = 0-12，条件是m + n <= 12，m和n <0中的至少一个; 且Z为或含有N，O或S亲核试剂官能团，或为或含有能够与N，O或S亲核试剂反应的官能团。 亲核体官能团包括-NH 2，-OH和-SH基团; 能够与这些官能团反应的基团包括-COCl，-COOCOR，-CONHNH 2，N-羟基琥珀酰亚胺酯，-NCS，-CHO，-COCH2I，亚磷酰胺和马来酰亚胺。
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公开(公告)号：US6136612A
公开(公告)日：2000-10-24
申请号：US43767
申请日：1998-06-18
IPC分类号： C09B23/01 , C09B23/02 , G01N33/533 , C07D209/56
CPC分类号： G01N33/533 , C09B23/0066 , C09B23/02 , Y10S436/80 , Y10S436/817
摘要： The present invention is directed to fluorescent dyes and its valence tautomers of the formula wherein Q represents a conjugated moiety that increases the fluorescent quantum yield of the compound; R.sub.1 is a functionalized group of the formula--(CH.sub.2).sub.j Y, wherein Y is selected from the group consisting of SO.sub.3 H, COOH, NH.sub.2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; R.sub.2 is a functionalized group of the formula --(CH.sub.2).sub.k Y', wherein Y' is selected from the group consisting of SO.sub.3 H, COOH, NH.sub.2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; M.sup.+ is a counterion selected from the group consisting of ammonium, alkali metal cations, and alkaline earth metal cations; n=1 to 4; m=1 to 4; j=2 to 10; and k=2 to 10.
摘要翻译： PCT No.PCT / EP96 / 04377 371日期：1998年5月11日 102（e）日期1998年5月11日PCT提交1996年10月8日PCT公布。 公开号WO97 / 13810PC。 日期1997年04月17日本发明涉及下式的荧光染料及其价态互变异构体：其中Q表示增加化合物的荧光量子产率的共轭部分; R 1是式 - （CH 2）j Y的官能化基团，其中Y选自SO 3 H，COOH，NH 2，CHO，NCS，环氧，邻苯二甲酰亚氨基和COOZ，其中Z表示离去基团; R2是式 - （CH2）kY'的官能化基团，其中Y'选自SO 3 H，COOH，NH 2，CHO，NCS，环氧，邻苯二甲酰亚氨基和COOZ，其中Z表示离去基团; M +是选自铵，碱金属阳离子和碱土金属阳离子的抗衡离子; n = 1〜4; m = 1〜4; j = 2〜10; k = 2〜10。
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公开(公告)号：US5897548A
公开(公告)日：1999-04-27
申请号：US744497
申请日：1996-11-07
CPC分类号： B01D19/0404
摘要： A method for reducing foam in a blood substance comprising contacting at least a portion of the blood substance with a contact-generating substrate which comprises an antifoaming agent, wherein the antifoaming agent comprises a triglyceride of one or more saturated or unsaturated C.sub.14 -C.sub.24 fatty acids or a mixture of triglycerides having one or more saturated or unsaturated C.sub.14 -C.sub.24 fatty acids, wherein the triglyceride or mixture of triglycerides has a hydrophilic-lipophilic balance of from 5 to 10.
摘要翻译： 一种用于减少血液中泡沫的方法，包括将至少一部分血液物质与包含消泡剂的接触产生底物接触，其中消泡剂包含一种或多种饱和或不饱和C 14 -C 24脂肪酸的甘油三酯 或具有一种或多种饱和或不饱和C 14 -C 24脂肪酸的甘油三酯的混合物，其中甘油三酸酯或甘油三酯的混合物具有5至10的亲水亲油平衡。
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公开(公告)号：US07803573B2
公开(公告)日：2010-09-28
申请号：US12071333
申请日：2008-02-20
申请人： Leopoldo Della Ciana
发明人： Leopoldo Della Ciana
IPC分类号： C12Q1/28
CPC分类号： C12Q1/28 , G01N33/582 , Y10S435/975
摘要： Method for increasing the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and a mediator of electrons through the use of a hypernucleophilic acylation catalyst belonging to the class of 4-aminopyridines. It is also described the use in diagnostic assays of chemiluminescent substrates containing said catalysts.
摘要翻译： 通过使用属于4-氨基吡啶类的超亲核酰化催化剂来增加包括鲁米诺，过氧化物酶，氧化剂和电子介体的化学发光反应的光的发射的方法。 还描述了在含有所述催化剂的化学发光底物的诊断测定中的用途。
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15.发明授权公开(公告)号：US06740755B2
公开(公告)日：2004-05-25
申请号：US09995350
申请日：2001-11-27
IPC分类号： C07D23924
CPC分类号： C09B23/06 , C09B23/083
摘要： A process for preparing an asymmetrical indocyanine dye comprising the steps of: a) reacting a first quaternised indolenine or substituted indolenine with a compound of the formula (II) or hydrochloride thereof, wherein n is 0 or 1 Ph is phenyl or substituted phenyl X is hydrogen, halogen or alkyl, preferably chlorine, in a solvent selected from the group consisting of acetic acid, acetic anhydride and mixtures thereof in the presence of acetyl chloride, to obtain an intermediate hemicyanine, and b) further reacting said intermediate hemicyanine with a second quaternised indolenine or substituted indolenine different from said first indolenine.
摘要翻译： 一种制备不对称吲哚菁染料的方法，包括以下步骤：a）使第一季铵化的吲哚宁或取代的吲哚宁与式（II）化合物或其盐酸盐反应，其中n为0或1 Ph为苯基或取代的苯基X为 氢，卤素或烷基，优选氯，在乙酰氯存在下，在选自乙酸，乙酸酐及其混合物的溶剂中，得到中间体半花青碱，和b）进一步使所述中间体半花青与第二季铵化反应 吲哚宁或取代的吲哚宁不同于所述第一种吲哚宁。
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公开(公告)号：US06251142B1
公开(公告)日：2001-06-26
申请号：US08987365
申请日：1997-12-09
IPC分类号： A61F206
CPC分类号： A61F2/82 , A61L31/10 , A61L31/16 , A61L2300/00 , C08L89/00
摘要： The apparatus comprises an implantation device having a body, the surface of which has at least a portion coated with a receptor, and a preparation containing a ligand formed from the combination of an active principle with a substance capable of binding to the receptor. This preparation may be brought into contact when desired with the implantation device which is, for example, a stent.
摘要翻译： 该装置包括具有主体的植入装置，其主体表面具有至少部分被受体覆盖的部分，以及包含由活性成分与能够结合受体的物质组合形成的配体的制剂。 当需要时，该制剂可以与例如支架的植入装置接触。
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公开(公告)号：US5811236A
公开(公告)日：1998-09-22
申请号：US468524
申请日：1995-06-06
申请人： Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
发明人： Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
IPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N33/533 , G01N33/569 , G01N33/58 , G01N33/76 , C07H21/04 , C07K15/28 , G01N23/53
CPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , G01N2458/30 , Y10S435/81
摘要： Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.
摘要翻译： 具有式[Re（P）m（L1）n（L2）o（L3）p（L4）q（L5）r（L6）s] t（B）u的电化学发光部分，其中P是Re的多齿配体; L1，L2，L3，L4，L5和L6是Re的配体，其各自可以彼此相同或不同; B是作为Re的配体或与P，L1，L2，L3，L4，L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L1，L2，L3，L4，L5，L6和B具有这样的组成和数量，可以诱导化学部分发射电磁辐射，并且由Re的配体提供的Re的总数相等于 Re的协调被披露。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。
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公开(公告)号：US08129136B2
公开(公告)日：2012-03-06
申请号：US12805827
申请日：2010-08-20
申请人： Leopoldo Della Ciana
发明人： Leopoldo Della Ciana
IPC分类号： C12Q1/28
CPC分类号： C12Q1/28 , G01N33/582 , Y10S435/975
摘要： The present invention relates to a kit for performing an assay. The kit comprises luminol, an oxidant, an electron mediator and a hypernucleophilic acylation catalyst.
摘要翻译： 本发明涉及用于进行测定的试剂盒。 试剂盒包括鲁米诺，氧化剂，电子介体和超亲核酰化催化剂。
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19.发明申请Active Particles for Bio-Analytical Applications and Methods for Preparation Thereof 审中-公开用于生物分析应用的活性颗粒及其制备方法公开(公告)号：US20110262356A1
公开(公告)日：2011-10-27
申请号：US13056700
申请日：2009-07-31
申请人： Sara Bonacchi , Ricarrdo Juris , Marco Montalti , Luca Prodi , Enrico Rampazzo , Nelsi Zaccheroni , Leopoldo Della Ciana , Serena Fabbroni , Stafano Grilli , Ettore Marzocchi
发明人： Sara Bonacchi , Ricarrdo Juris , Marco Montalti , Luca Prodi , Enrico Rampazzo , Nelsi Zaccheroni , Leopoldo Della Ciana , Serena Fabbroni , Stafano Grilli , Ettore Marzocchi
IPC分类号： A61K49/00 , A61K9/14 , A61K31/352 , A61K31/403 , A61K31/555 , A61K31/351 , A61K31/695 , A61K31/538 , B82Y5/00
CPC分类号： A61K49/0032 , A61K49/0093 , C07F7/21
摘要： Luminescent and/or electroactive nanoparticles suitable for MRI (Magnetic Resonance Imaging) and/or PET (Positron Emission Tomography) are prepared by mixing luminescent or electroactive compounds and ethyl oxide/propyl oxide block-copolymers in an organic solvent, which is thereafter evaporated in order to obtain a residue; and by hydrolyzing-condensating tetraalkoxysilanes in an aqueous solution in the presence of the residue; the obtained nanoparticles show no or a negligible release of the luminescent or electroactive compounds and are useful for bio-analytic and bio-medic applications.
摘要翻译： 通过将有机溶剂中的发光或电活性化合物和乙基氧化物/丙基氧化物嵌段共聚物混合，然后将其蒸发而制备适用于MRI（磁共振成像）和/或PET（正电子发射断层成像）的发光和/或电活性纳米颗粒 为了获得残留物; 并通过在残留物存在下在水溶液中水解缩合四烷氧基硅烷; 所获得的纳米颗粒显示发光或电活性化合物没有或可忽略的释放，并且可用于生物分析和生物医学应用。
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公开(公告)号：US20110053200A1
公开(公告)日：2011-03-03
申请号：US12805827
申请日：2010-08-20
申请人： Leopoldo Della Ciana
发明人： Leopoldo Della Ciana
IPC分类号： C12Q1/28
CPC分类号： C12Q1/28 , G01N33/582 , Y10S435/975
摘要： Method for increasing the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and a mediator of electrons through the use of a hypernucleophilic acylation catalyst belonging to the class of 4-aminopyridines. It is also described the use in diagnostic assays of chemiluminescent substrates containing said catalysts.
摘要翻译： 通过使用属于4-氨基吡啶类的超亲核酰化催化剂来增加包括鲁米诺，过氧化物酶，氧化剂和电子介体的化学发光反应的光的发射的方法。 还描述了在含有所述催化剂的化学发光底物的诊断测定中的用途。
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