公开(公告)号：US20240167079A1

公开(公告)日：2024-05-23

申请号：US18520514

申请日：2023-11-27

Applicant: 10X GENOMICS, INC.

Inventor： Xinying ZHENG ,  Serge SAXONOV ,  Michael SCHNALL-LEVIN ,  Kevin NESS ,  Rajiv BHARADWAJ

IPC: C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

CPC classification number: C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

Abstract: The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.

公开(公告)号：US20240035066A1

公开(公告)日：2024-02-01

申请号：US18346011

申请日：2023-06-30

Applicant: 10X Genomics, Inc.

Inventor： Mirna JAROSZ ,  Michael SCHNALL-LEVIN ,  Serge SAXONOV ,  Benjamin J. HINDSON ,  Xinying ZHENG

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6837

CPC classification number: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6837

Abstract: The present invention is directed to methods, compositions and systems for capturing and analyzing sequence information contained in targeted regions of a genome. Such targeted regions may include exomes, partial exomes, introns, combinations of exonic and intronic regions, genes, panels of genes, and any other subsets of a whole genome that may be of interest.

公开(公告)号：US20230323427A1

公开(公告)日：2023-10-12

申请号：US18131800

申请日：2023-04-06

Applicant: 10x Genomics, Inc.

Inventor： Michael SCHNALL-LEVIN

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6874

Abstract: Provided herein in some aspects are methods, compositions, kits, and systems for performing multiplexed single-cell analysis on an in situ platform, providing alternatives which have a higher cell throughput and/or lower cost per cell compared to current single-cell analysis techniques. In some embodiments, the methods disclosed herein comprise using labeling agents that comprise sample-specific barcodes and/or cell feature specific barcodes (e.g., analyte specific barcodes) to label single-cell populations, immobilizing the labeled cells, and performing in situ detection of the labeling agents and/or other features including cellular analytes of the labeled cells.

公开(公告)号：US20220228201A1

公开(公告)日：2022-07-21

申请号：US17565028

申请日：2021-12-29

Applicant: 10x Genomics, Inc.

Inventor： Geoffrey MCDERMOTT ,  David Michael PATTERSON ,  Andrew D. PRICE ,  Eswar Prasad RAMACHANDRAN IYER ,  Michael SCHNALL-LEVIN

IPC: C12Q1/6837 ,  C12Q1/6869

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, provided herein is photocontrollable hybridization, where oligonucleotides with photo-caged bases prevent hybridization of complementary nucleic acid strands. Uncaging of the oligonucleotides allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.