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    SYSTEMS AND METHODS FOR PROCESSING NUCLEIC ACID MOLECULES FROM ONE OR MORE CELLS
    1.
    发明申请
    SYSTEMS AND METHODS FOR PROCESSING NUCLEIC ACID MOLECULES FROM ONE OR MORE CELLS 有权

    公开(公告)号：US20250059588A1

    公开(公告)日：2025-02-20

    申请号：US18818492

    申请日：2024-08-28

    Applicant: 10x Genomics, Inc.

    Inventor： Michael SCHNALL-LEVIN

    IPC: C12Q1/6806 ,  C12Q1/6874

    Abstract: The present disclosure provides methods and systems for processing nucleic acid molecules from one or more cells. A cell may be comprised in a cell bead. Processing may comprise uniquely identifying nucleic acid molecules. Nucleic acid molecules may be identified by barcoding. Barcoding may be combinatorial barcoding. Combinatorial barcoding may allow for generation of a large number of barcodes, thereby uniquely identifying nucleic acids from a large number of single cells. Combinatorial barcoding may be performed in successive operations. Successive operations may be performed in partitions.

    COMPOSITIONS AND METHODS FOR IN SITU SEQUENCING
    3.
    发明公开
    COMPOSITIONS AND METHODS FOR IN SITU SEQUENCING 审中-公开

    公开(公告)号：US20240084378A1

    公开(公告)日：2024-03-14

    申请号：US18196333

    申请日：2023-05-11

    Applicant: 10x Genomics, Inc.

    Inventor： Patrick J. MARKS ,  Michael SCHNALL-LEVIN

    IPC: C12Q1/6874 ,  C12Q1/6841

    CPC classification number: C12Q1/6874 ,  C12Q1/6841 ,  C12Q1/682

    Abstract: Methods and compositions for performing base-by-base sequencing in situ in a cell or tissue sample that minimize optical crowding are described. In some embodiments, a sequencing primer hybridizes to a priming site 3′ to an identifier sequence (e.g., a barcode sequence) in the sample such that the sequencing primer can be extended by a polymerase in a base-by-base fashion using the identifier sequence as a template. The sample can be contacted with nucleotides in a cyclic series of nucleotide incorporation or binding steps, and signals indicative of the incorporation or binding events can be detected to generate signal code sequences comprising a series of signal codes (corresponding to signals (ON signals), absence of signals (OFF signals), or a combination thereof) detected in the sequential cycles. Decoding of the identifier sequences based at least in part of the signal code sequences can be used to detect and locate the corresponding analytes.

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