公开(公告)号：US20240326054A1

公开(公告)日：2024-10-03

申请号：US18427317

申请日：2024-01-30

Applicant: 10x Genomics, Inc.

Inventor： Mohammad RAHIMI LENJI ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  B01F23/411 ,  B01L3/02

CPC classification number: B01L3/502784 ,  B01L3/021 ,  B01F23/411 ,  B01L2200/0673 ,  B01L2300/0867

Abstract: The invention provides kits, devices, methods, and systems for forming droplets or particles and methods of their use. The devices may be used to form droplets of a size suitable for utilization as microscale chemical reactors, e.g., for genetic sequencing. In general, droplets are formed in a device by flowing a first liquid through a channel and into a droplet formation region including a second liquid, i.e., the continuous phase. The invention allows for more efficient recovery of droplets or processed droplets.

公开(公告)号：US20230278038A1

公开(公告)日：2023-09-07

申请号：US18177562

申请日：2023-03-02

Applicant: 10x Genomics, Inc.

Inventor： Mohammad RAHIMI LENJI ,  Yiran ZHANG ,  Francis MARCOGLIESE ,  Tobias Daniel WHEELER ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011

CPC classification number: B01L3/502792 ,  B01F23/4144 ,  B01F33/3011 ,  B01F23/4105 ,  B01L2300/0867 ,  B01L2200/16 ,  B01L2200/0673 ,  B01L2300/0816

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. Devices use flow focusing arrangements of channels to produce droplets. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20190060905A1

公开(公告)日：2019-02-28

申请号：US15977824

申请日：2018-05-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F13/00

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20240326052A1

公开(公告)日：2024-10-03

申请号：US18738471

申请日：2024-06-10

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Kevin Ness ,  Debkishore Mitra ,  Donald A. MASQUELIER ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  B01L3/02 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N2035/00237 ,  G01N35/085 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US20230356231A1

公开(公告)日：2023-11-09

申请号：US18223903

申请日：2023-07-19

Applicant: 10x Genomics, Inc.

Inventor： Michael John TIERNEY ,  Bill Kengli LIN ,  Rajiv VERMA ,  Lynna CHEN ,  Kevin GILMARTIN ,  Rajiv BHARADWAJ

IPC: B01L7/00 ,  B01L3/00

CPC classification number: B01L7/52 ,  B01L3/502707

Abstract: The invention provides methods for rapid setting, adjusting, and controlling the temperature of a microfluidic device.

公开(公告)号：US20230324421A1

公开(公告)日：2023-10-12

申请号：US18132115

申请日：2023-04-07

Applicant: 10x Genomics, Inc.

Inventor： Yiran ZHANG ,  Felice Alessio BAVA ,  Rhyan Blaine DOCKTER ,  Rajiv BHARADWAJ

IPC: G01N35/00 ,  B01L3/00

CPC classification number: G01N35/00029 ,  B01L3/502715 ,  G01N2035/00168 ,  B01L2200/0689 ,  B01L2200/025 ,  B01L2300/0627 ,  B01L2200/16

Abstract: Disclosed herein are systems for receiving a biological sample for analysis and methods for preparing and/or analyzing a biological sample. In various embodiments, a system includes a first layer comprising a first region configured to receive a sample, a gasket disposed on the first layer such that the gasket surrounds the first region, and a housing layer. The first layer is disposed between the gasket and the housing layer. The system further includes a clamp configured to apply a force on the gasket to form a liquid tight seal against the first layer.

公开(公告)号：US20220193667A1

公开(公告)日：2022-06-23

申请号：US17563645

申请日：2021-12-28

Applicant: 10x Genomics, Inc.

Inventor： Tobias Daniel WHEELER ,  Rajiv BHARADWAJ

IPC: B01L3/00

Abstract: A microfluidic system includes a microfluidic chip having a plurality of fluid channels, each fluid channel having an opening providing access to an interior of the fluid channel, and a gasket disposable on the microfluidic chip in an aligned configuration, the gasket including a first side configured to face the microfluidic chip in the aligned configuration, a second side opposite the first side, and an aperture extending through the gasket from the first side to the second side, the aperture being sized and positioned to allow a communication of pressure from the second side of the gasket to the openings of at least two fluid channels when the gasket is in the aligned configuration.

公开(公告)号：US20210205811A1

公开(公告)日：2021-07-08

申请号：US17144575

申请日：2021-01-08

Applicant: 10X Genomics, Inc.

Inventor： Alireza SALMANZADEH ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  G01N29/22

Abstract: Devices, systems, and their methods of use, for sorting droplets or particles are provided. A source of acoustic energy can be employed to sort droplets or particles of a desired and predictable property.

公开(公告)号：US20200114358A1

公开(公告)日：2020-04-16

申请号：US16706313

申请日：2019-12-06

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F5/04 ,  B01F11/02 ,  B01F13/00 ,  B01F3/08 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6806 ,  B01L3/02

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20190060906A1

公开(公告)日：2019-02-28

申请号：US15977861

申请日：2018-05-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08

CPC classification number: B01L3/0241 ,  B01F3/0807 ,  B01F3/0811 ,  B01F5/0473 ,  B01F5/0478 ,  B01F5/048 ,  B01F11/0241 ,  B01F13/0062 ,  B01F13/0071 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0851 ,  B01L2300/161 ,  B01L2400/086 ,  C12N2533/50 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2563/185 ,  C40B40/06 ,  G01N33/54313 ,  G01N33/58 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.