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    PROTEIN PURIFICATION PROCESS
    11.
    发明申请
    PROTEIN PURIFICATION PROCESS 审中-公开
    蛋白纯化过程

    公开(公告)号：US20160115194A1

    公开(公告)日：2016-04-28

    申请号：US14894993

    申请日：2014-02-27

    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    Inventor： Peter Stanley GAGNON

    IPC: C07K1/36 ,  C07K16/32 ,  C07K16/00

    CPC classification number: C07K1/36 ,  C07K1/30 ,  C07K1/34 ,  C07K16/00 ,  C07K16/32

    Abstract: A method of purifying a target protein includes contacting a cell culture harvest or a protein preparation including at least one target protein with at least one fatty acid having 8 to 10 carbon atoms to form a mixture, contacting the mixture with one or more solids to form a mixture, the one or more solids comprise a cationic functional group, a metal binding functional group, or both, the metal binding functional group including a nitrogen-containing moiety selected from (1) a polyamine, (2) an imine, (3) an N-heterocycle, (4) an amino acid, (5) an N-hydroxyamide, (6), an arylamine, and combinations thereof, and separating solid materials after contacting the mixture with the one or more solids to provide a solution comprising the target protein.

    Abstract translation: 纯化靶蛋白的方法包括将细胞培养物收获物或包含至少一种靶蛋白质的蛋白质制剂与至少一种具有8至10个碳原子的脂肪酸接触以形成混合物，使该混合物与一种或多种固体接触以形成 一种或多种固体包含阳离子官能团，金属结合官能团或两者，所述金属结合官能团包括选自（1）多胺，（2）亚胺，（3） ）N-杂环，（4）氨基酸，（5）N-羟基酰胺，（6），芳基胺及其组合，并且在将混合物与一种或多种固体接触后分离固体材料以提供溶液 包含靶蛋白。

    MIXED MULTIFUNCTIONAL METAL AFFINITY SURFACES FOR REDUCING AGGREGATE CONTENT IN PROTEIN PREPARATIONS
    13.
    发明申请
    MIXED MULTIFUNCTIONAL METAL AFFINITY SURFACES FOR REDUCING AGGREGATE CONTENT IN PROTEIN PREPARATIONS 审中-公开
    用于降低蛋白质制备中的聚集含量的混合多功能金属亲合表面

    公开(公告)号：US20160001262A1

    公开(公告)日：2016-01-07

    申请号：US14766126

    申请日：2014-02-05

    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    Inventor： Peter Stanley GAGNON

    IPC: B01J20/24 ,  B01J20/288 ,  B01D15/38 ,  C07K1/16

    CPC classification number: B01J20/24 ,  B01D15/3828 ,  B01D15/3847 ,  B01J20/288 ,  B01J20/3251 ,  B01J20/3285 ,  B01J20/3293 ,  B01J39/26 ,  B01J41/20 ,  B01J2220/80 ,  C07K1/165 ,  B01D15/327

    Abstract: A composition for reducing the aggregate content of a protein preparation includes a first substrate having a first surface-bound ligand possessing a metal affinity functionality, the metal affmity functionality being substantially devoid of a metal, and an additional surface-bound ligand different from the first surface-bound ligand, the additional surface-bound ligand having an aggregate charge not opposite to that of the metal affinity functionality, optionally the additional surface-bound ligand is provided on an additional substrate such that the composition comprises a mixture of the first substrate and the additional substrate.

    Abstract translation: 用于降低蛋白质制剂的骨料含量的组合物包括具有第一表面结合配体的第一底物，其具有金属亲和功能，金属亲和官能团基本上不含金属，以及不同于第一 表面结合的配体，另外的表面结合配体具有与金属亲和官能团不相反的聚集体电荷，任选地附加的表面结合配体提供在另外的基底上，使得组合物包含第一底物和 附加底物。

    METHODS FOR REDUCING AGGREGATE LEVELS IN PROTEIN PREPARATIONS BY TREATMENT WITH THIO-HETEROCYCLIC CATIONS
    14.
    发明申请
    METHODS FOR REDUCING AGGREGATE LEVELS IN PROTEIN PREPARATIONS BY TREATMENT WITH THIO-HETEROCYCLIC CATIONS 有权
    通过用THIO-HETEROCYCLIC治疗来降低蛋白质制剂中聚集体水平的方法

    公开(公告)号：US20150376230A1

    公开(公告)日：2015-12-31

    申请号：US14766123

    申请日：2014-02-05

    Applicant: AGENCY FOR SCIENCE TECHNOLOGY AND RESEARCH

    Inventor： Peter Stanley GAGNON

    IPC: C07K1/14 ,  C07K16/00

    CPC classification number: C07K1/14 ,  B01D15/327 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3828 ,  C07K1/165 ,  C07K1/20 ,  C07K16/00 ,  C07K16/3015

    Abstract: A method of reducing the aggregate content in a protein preparation having a target protein includes contacting the protein preparation with a thio-heterocyclic cation to form a mixture, contacting the mixture with at least one functionalized solid to remove excess thio-heterocyclic cations; and optionally contacting the mixture, simultaneously or sequentially, with at least one further functionalized solid to further reduce aggregate content of the protein preparation.

    Abstract translation: 降低具有靶蛋白质的蛋白质制剂中骨料含量的方法包括使蛋白制剂与硫杂环阳离子接触以形成混合物，使混合物与至少一种官能化固体接触以除去过量的硫杂环阳离子; 并任选地将所述混合物同时或顺序地与至少一种其它官能化固体接触，以进一步降低蛋白质制剂的聚集体含量。

    ANTIBODY PURIFICATION PROCESS
    17.
    发明申请
    ANTIBODY PURIFICATION PROCESS 审中-公开
    抗体纯化方法

    公开(公告)号：US20160362446A1

    公开(公告)日：2016-12-15

    申请号：US15121676

    申请日：2014-02-27

    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    Inventor： Peter Stanley GAGNON

    IPC: C07K1/30 ,  C07K16/00

    CPC classification number: C07K1/30 ,  C07K1/32 ,  C07K16/00

    Abstract: A method of purifying a target antibody includes contacting a cell culture harvest or a protein preparation including at least one target antibody with at least one fatty acid having 7 to 10 carbon atoms to form a mixture, contacting this mixture with allantoin, and then separating solid materials to provide a solution comprising the target antibody. Solid materials can be removed by filtration, sedimentation or centrifugation, and the fatty acids can be enanthic, caprylic, pelargonic, nonenoic or capric acid. The invention is also directed to kits used to facilitate this method of antibody purification.

    Abstract translation: 纯化靶抗体的方法包括将细胞培养物收获物或包含至少一种靶抗体的蛋白质制剂与至少一种具有7至10个碳原子的脂肪酸接触以形成混合物，使该混合物与尿囊素接触，然后分离固体 提供包含靶抗体的溶液的材料。 固体材料可以通过过滤，沉淀或离心去除，脂肪酸可以是甘油，辛酸，壬酸，壬烯或癸酸。 本发明还涉及用于促进抗体纯化方法的试剂盒。

    PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AND ELECTROPOSITIVE SURFACES
    18.
    发明申请
    PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AND ELECTROPOSITIVE SURFACES 审中-公开
    非离子有机聚合物和电镀表面存在的蛋白质纯化

    公开(公告)号：US20160009759A1

    公开(公告)日：2016-01-14

    申请号：US14769101

    申请日：2014-02-26

    Applicant: Agency for Science, Technology and Research

    Inventor： Peter Stanley GAGNON

    IPC: C07K1/16

    CPC classification number: C07K1/165 ,  B01D15/305 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/30 ,  C07K1/303 ,  B01D15/327 ,  B01D15/34

    Abstract: A method of purifying a desired protein from a preparation includes (a) providing the preparation in a form having less than about 5% of chromatin residing in an original production medium, (b) contacting the preparation with a nonionic organic polymer and a salt, a concentration of nonionic organic polymer being sufficient to precipitate the desired protein or cause its accretion on a hydrophilic surface, or maintain it in a precipitated state or accreted on the hydrophilic surface, the salt concentration being sufficient to produce greater than physiological conductivity, and (c) contacting the preparation with at least one electropositive surface, optionally in the presence of a salt concentration sufficient to produce greater than physiological conductivity, the desired protein does not substantially adsorb to the at least one electropositive surface while not preventing adsorption of acidic contaminants to the at least one electropositive surface.

    Abstract translation: 从制剂中纯化所需蛋白质的方法包括（a）提供具有少于约5％染色质残留在原始生产培养基中的形式的制剂，（b）使制剂与非离子有机聚合物和盐接触， 非离子有机聚合物的浓度足以沉淀所需蛋白质或使其在亲水表面上的沉积，或将其保持在沉淀状态或在亲水表面上增加，盐浓度足以产生大于生理电导率，和（ c）任选地在足以产生大于生理电导率的盐浓度的情况下任选地将制剂与至少一个正电性表面接触，所需蛋白质基本上不吸附至至少一个正电面，同时不阻止酸性污染物的吸附 所述至少一个正电面。

    MATERIALS AND METHODS FOR REMOVING ENDOTOXINS FROM PROTEIN PREPARATIONS
    19.
    发明申请
    MATERIALS AND METHODS FOR REMOVING ENDOTOXINS FROM PROTEIN PREPARATIONS 有权
    从蛋白质制备中去除内毒素的材料和方法

    公开(公告)号：US20160002290A1

    公开(公告)日：2016-01-07

    申请号：US14769245

    申请日：2014-02-20

    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    Inventor： Peter Stanley GAGNON ,  Vincent VAGENENDE

    IPC: C07K1/36 ,  C07K1/34 ,  C07K1/30 ,  C07K1/18

    CPC classification number: C07K1/36 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/18 ,  C07K1/30 ,  C07K1/34

    Abstract: A method includes (i) adding allantoin in a supersaturating amount to a protein preparation including a desired protein and at least one endotoxin as a contaminant, (ii) removing solids after the adding step to provide a sample for further purification by void exclusion chromatography on a packed particle bed of electropositive particles in a column, the packed particle bed having an interparticle volume, (iii) applying a sample volume to the packed particle bed, wherein the electropositive particles support void exclusion chromatography, and wherein the sample volume is not greater than the interparticle volume, and (iv) eluting a purified sample including the desired protein and a reduced amount of the endotoxin. The method is optionally carried out with only the allantoin treatment or only the void exclusion chromatography.

    Abstract translation: 一种方法包括（i）将超滤量的尿囊素加入到包含所需蛋白质和至少一种作为污染物的内毒素的蛋白质制剂中，（ii）在加入步骤后除去固体，以提供样品进一步通过空隙排阻色谱法进行纯化 在柱中的正电性颗粒的填充颗粒床，所述填充颗粒床具有颗粒间体积，（iii）将样品体积施加到所述填充颗粒床，其中所述正电荷颗粒支持空隙排阻层析，并且其中所述样品体积不大 （iv）洗脱含有所需蛋白质和减少量内毒素的纯化样品。 该方法任选仅通过尿囊素处理或仅进行空白排阻色谱进行。

    PROTEIN PURIFICATION METHODS
    20.
    发明申请
    PROTEIN PURIFICATION METHODS 有权
    蛋白纯化方法

    公开(公告)号：US20150376231A1

    公开(公告)日：2015-12-31

    申请号：US14766131

    申请日：2014-02-05

    Applicant: AGENCY FOR SCIENCE TECHNOLOGY AND RESEARCH

    Inventor： Peter Stanley GAGNON

    IPC: C07K1/30 ,  C07K16/00 ,  B01D15/36 ,  C07K16/28

    CPC classification number: C07K1/303 ,  B01D15/36 ,  C07K1/30 ,  C07K16/00 ,  C07K16/2863 ,  C07K16/32

    Abstract: A method for the purification of a desired protein from a protein preparation includes conditioning the protein preparation by treatment with soluble organic multivalent ions, immobilized organic multivalent ions, or both, optionally in the presence of supersaturated allantoin, thereby removing at least 90% of chromatin, then (1) precipitating the desired protein with a nonionic organic polymer in the presence of non-protein-precipitating salts at greater than physiological concentration to provide a precipitate of the desired protein; or (2) precipitating the desired protein with a nonionic organic polymer in the absence of non-precipitating salts at greater than physiological concentration to provide a precipitate and subsequently washing the precipitate with a nonionic organic polymer in the presence of non-protein-precipitating salts at greater than physiological concentration.

    Abstract translation: 用于从蛋白质制剂中纯化所需蛋白质的方法包括通过用可溶性有机多价离子，固定的有机多价离子或两者处理，任选地在过饱和尿囊素存在下调节蛋白质制剂，由此除去至少90％的染色质 ，然后（1）在非蛋白质沉淀盐存在下，用非离子有机聚合物在大于生理浓度下沉淀所需蛋白质以提供所需蛋白质的沉淀物; 或（2）在不存在非沉淀盐的情况下，用非离子有机聚合物在大于生理浓度下沉淀所需蛋白质以提供沉淀物，随后在非蛋白质沉淀盐存在下用非离子有机聚合物洗涤沉淀物 大于生理浓度。

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