公开(公告)号：US20160257944A1

公开(公告)日：2016-09-08

申请号：US15159140

申请日：2016-05-19

Applicant: AJINOMOTO CO., INC.

Inventor： Kunio NAKATA ,  Yoshinori Tajima ,  Uno Tagami ,  Takashi Oku ,  Yasuhiro Kashima

IPC: C12N9/88 ,  C08K3/22 ,  C08K3/04 ,  C12P5/00 ,  C08F36/08

CPC classification number: C12N9/88 ,  C08F36/08 ,  C08K3/04 ,  C08K3/22 ,  C08K2003/2296 ,  C12N15/09 ,  C12P5/007 ,  C12P5/026 ,  C12Y402/03007 ,  C12Y402/03027 ,  C08L9/00

Abstract: Modified isoprene synthases that have one or more mutations of a given amino acid residue(s) in any amino acid sequence of (a) the amino acid sequence of SEQ ID NO:4, (b) an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO:4, or (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:4, and has an isoprene synthetic activity are useful for preparing isoprene monomer in improved yields.

Abstract translation: 在（a）SEQ ID NO：4的氨基酸序列的任何氨基酸序列中具有一个或多个给定氨基酸残基的一个或多个突变的修饰的异戊二烯合酶，​​（b）具有一个或几个氨基酸的氨基酸序列 在SEQ ID NO：4的氨基酸序列中的酸取代，缺失，插入或添加，或（c）与SEQ ID NO：4的氨基酸序列具有90％或更高同一性的氨基酸序列，并且具有异戊二烯 合成活性可用于以提高的产率制备异戊二烯单体。

公开(公告)号：US20150259717A1

公开(公告)日：2015-09-17

申请号：US14723776

申请日：2015-05-28

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC: C12P13/14

CPC classification number: C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

Abstract translation: 具有羧基的酸性物质是通过在培养基中培养已被改良以增强ybjL基因表达的微生物，并从培养基中收集具有羧基的酸性物质产生的。