公开(公告)号：US09822385B2

公开(公告)日：2017-11-21

申请号：US14723776

申请日：2015-05-28

申请人： AJINOMOTO CO., INC.

发明人： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

CPC分类号： C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

摘要： An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

公开(公告)号：US20150203881A1

公开(公告)日：2015-07-23

申请号：US14668080

申请日：2015-03-25

申请人： AJINOMOTO CO., INC.

发明人： Leonid Romanovich Ptitsyn ,  Irina Borisovna Altman ,  Veronika Aleksandrovna Kotliarova ,  Olga Nikolaevna Mokhova ,  Tatyana Abramovna Yampolskaya ,  Yury Ivanovich Kozlov ,  Masaru Terashita ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/08 ,  C12P13/06

CPC分类号： C12P13/08 ,  C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24 ,  C12Y101/01002

摘要： A method for producing an L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of a wild-type alcohol dehydrogenase encoded by the adhE gene or a mutant alcohol dehydrogenase which is resistant to aerobic inactivation.

摘要翻译： 例如使用肠杆菌科的细菌来描述L-氨基酸的制造方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸，L-色氨酸或L-谷氨酸 家族，其中细菌已经被修饰以增强由adhE基因编码的野生型醇脱氢酶的活性或对有氧失活有抗性的突变型醇脱氢酶。

公开(公告)号：US20150259717A1

公开(公告)日：2015-09-17

申请号：US14723776

申请日：2015-05-28

申请人： AJINOMOTO CO., INC.

发明人： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/14

CPC分类号： C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

摘要： An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

摘要翻译： 具有羧基的酸性物质是通过在培养基中培养已被改良以增强ybjL基因表达的微生物，并从培养基中收集具有羧基的酸性物质产生的。

公开(公告)号：US20180044703A1

公开(公告)日：2018-02-15

申请号：US15787861

申请日：2017-10-19

申请人： AJINOMOTO CO., INC.

发明人： Yoshihiko Hara ,  Keita Fukui ,  Yoshinori Tajima ,  Kazue Kawamura ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/14 ,  C12P7/50 ,  C12P7/48 ,  C12P7/46 ,  C07K14/265 ,  C07K14/245 ,  C12P13/20 ,  C07K14/195

CPC分类号： C12P13/14 ,  C07K14/195 ,  C07K14/245 ,  C07K14/265 ,  C12P7/46 ,  C12P7/48 ,  C12P7/50 ,  C12P13/20

摘要： An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

公开(公告)号：US20130288313A1

公开(公告)日：2013-10-31

申请号：US13938601

申请日：2013-07-10

申请人： Ajinomoto Co., Inc.

发明人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/08

CPC分类号： C12P13/08 ,  C12P13/04

摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

摘要翻译： 通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生， 在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。

公开(公告)号：US20140045228A1

公开(公告)日：2014-02-13

申请号：US14027365

申请日：2013-09-16

申请人： AJINOMOTO CO., INC.

发明人： Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/08

CPC分类号： C12P13/08 ,  C12P13/04 ,  C12P13/14 ,  C12P13/227

摘要： An L-amino acid is produced by culturing an Enterobacteriaceae which is able to produce an L-amino acid in a medium containing glycerol, especially crude glycerol, as the carbon source to produce and accumulate the L-amino acid in the culture, and collecting the L-amino acid from the culture.

摘要翻译： 通过培养能够在含有甘油，特别是粗甘油的培养基中产生L-氨基酸的肠杆菌科生产L-氨基酸作为碳源，以在培养物中产生和积累L-氨基酸，并收集 来自培养物的L-氨基酸。