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公开(公告)号：US09157127B2
公开(公告)日：2015-10-13
申请号：US14183113
申请日：2014-02-18
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q1/689 , C12Q2600/158 , G01N21/6428 , G01N21/6456 , G01N2021/6432 , Y02A50/53 , C12Q2521/507 , C12Q2522/101 , C12Q2527/137 , C12Q2565/629 , C12Q2563/155 , C12Q2563/159
摘要： A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.
摘要翻译： 一种方法包括提供包含重组酶，单链结合蛋白和一种或多种寡核苷酸的混合物; 并检测反应混合物中的颗粒。
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公开(公告)号：US20150240298A1
公开(公告)日：2015-08-27
申请号：US14705150
申请日：2015-05-06
申请人： Alere San Diego Inc.
发明人： Olaf Piepenburg , Niall A. Armes
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6806 , C12Q1/6848 , C12Q2549/101 , C12Q2527/125 , C12Q2521/507 , C12Q2527/137
摘要： This disclosure describes kits, reagents and methods for Recombinase Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed kits, reagents and methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
摘要翻译： 本公开描述了利用重组酶和相关蛋白质的特性的靶DNA的重组酶聚合酶扩增（RPA）的试剂盒，试剂和方法，以允许DNA聚合酶反应的序列特异性引发的单链同源DNA侵入双链DNA。 所公开的试剂盒，试剂和方法具有不需要热循环或嗜热酶的优点，因此相对于其它扩增方法提供了容易且价格实惠的实施和便携性。
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公开(公告)号：US08962255B2
公开(公告)日：2015-02-24
申请号：US14033679
申请日：2013-09-23
CPC分类号： C12Q1/6844 , C12Q1/6848 , C12Q1/686 , C12Q1/70 , C12Q2522/101 , C12Q2521/507 , C12Q2527/101 , C12Q2527/125 , C12Q2531/119
摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
摘要翻译： 本公开描述了利用重组酶和相关蛋白质的特性的靶DNA的重组酶 - 聚合酶扩增（RPA）的相关新方法，以允许DNA聚合酶反应的序列特异性引发的单链同源DNA侵入双链DNA。 所公开的方法具有不需要热循环或嗜热酶的优点。 此外，所公开的方法的改进的持续性可以允许将DNA扩增长达数百个百兆位。
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公开(公告)号：US20140141434A1
公开(公告)日：2014-05-22
申请号：US13911268
申请日：2013-06-06
发明人： Niall A. Armes , Derek L. Stemple
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6806 , C12Q1/6848 , C12Q1/686 , G06F17/30864 , G06F17/30902 , C12Q2521/507 , C12Q2522/101 , C12Q2549/119 , C12Q2527/101 , C12Q2531/119
摘要： This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length.
摘要翻译： 本公开描述了利用细菌RecA和相关蛋白质的性质的靶DNA的重组酶 - 聚合酶扩增（RPA）的三种相关新方法，以单链同源DNA侵入双链DNA，允许DNA聚合酶反应的序列特异性引发 。 所公开的方法具有不要求热循环或嗜热酶的优点。 此外，所公开的方法的改进的持续性允许DNA长达数百百万位碱基的扩增。
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公开(公告)号：US20200095584A1
公开(公告)日：2020-03-26
申请号：US16703190
申请日：2019-12-04
IPC分类号： C12N15/113 , C12Q1/6816 , C12Q1/6827 , C12Q1/6853 , C12Q1/6844 , C12Q1/6804 , G01N21/78 , G01N33/558
摘要： This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
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公开(公告)号：US09932577B2
公开(公告)日：2018-04-03
申请号：US14066334
申请日：2013-10-29
IPC分类号： C12Q1/68 , C12N15/113 , G01N21/78 , G01N33/558 , G01N21/77
CPC分类号： C12N15/113 , C12Q1/6804 , C12Q1/6816 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , G01N21/78 , G01N33/558 , G01N2021/7759 , G01N2021/7763 , G01N2021/7793 , C12Q2521/507 , C12Q2565/102 , C12Q2537/1376 , C12Q2565/101 , C12Q2525/119 , C12Q2521/301 , C12Q2561/113 , C12Q2537/125 , C12Q2525/125 , C12Q2563/131 , C12Q2565/625
摘要： This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
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公开(公告)号：US20170044598A1
公开(公告)日：2017-02-16
申请号：US15099754
申请日：2016-04-15
发明人： Niall A. Armes , Derek L. Stemple
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6806 , C12Q1/6848 , C12Q1/686 , G06F16/951 , G06F16/9574 , C12Q2521/507 , C12Q2522/101 , C12Q2549/119 , C12Q2527/101 , C12Q2531/119
摘要： This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length.
摘要翻译： 本公开描述了利用细菌RecA和相关蛋白质的性质的目标DNA的重组酶 - 聚合酶扩增（RPA）的三种相关新方法，以单链同源DNA侵入双链DNA，允许DNA聚合酶反应的序列特异性引发 。 所公开的方法具有不需要热循环或嗜热酶的优点。 此外，所公开的方法的改进的持续性允许DNA长达数百百万位碱基的扩增。
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公开(公告)号：US09309502B2
公开(公告)日：2016-04-12
申请号：US14610915
申请日：2015-01-30
申请人： Alere San Diego Inc.
CPC分类号： C12Q1/6844 , B01L7/52 , B01L2200/10 , B01L2300/0627 , B01L2300/18 , C12N9/1252 , C12Q1/6806 , C12Q1/6818 , C12Y207/07007 , G01N33/5308 , C12Q2531/113 , C12Q2522/101 , C12Q2521/507 , C12Q2527/125
摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.
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公开(公告)号：US20160097090A1
公开(公告)日：2016-04-07
申请号：US14966352
申请日：2015-12-11
IPC分类号： C12Q1/68 , G01N21/78 , G01N33/558
CPC分类号： C12N15/113 , C12Q1/6804 , C12Q1/6816 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , G01N21/78 , G01N33/558 , G01N2021/7759 , G01N2021/7763 , G01N2021/7793 , C12Q2521/507 , C12Q2565/102 , C12Q2537/1376 , C12Q2565/101 , C12Q2525/119 , C12Q2521/301 , C12Q2561/113 , C12Q2537/125 , C12Q2525/125 , C12Q2563/131 , C12Q2565/625
摘要： This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
摘要翻译： 本公开提供了用于快速多重RPA反应的方法和试剂以及用于检测多重RPA反应产物的改进方法。 此外，本公开提供了消除RPA过程之间的残留污染的新方法。
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公开(公告)号：US20160032374A1
公开(公告)日：2016-02-04
申请号：US14626504
申请日：2015-02-19
CPC分类号： C12Q1/6844 , C12Q1/6848 , C12Q1/686 , C12Q1/70 , C12Q2522/101 , C12Q2521/507 , C12Q2527/101 , C12Q2527/125 , C12Q2531/119
摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
摘要翻译： 本公开描述了利用重组酶和相关蛋白质的特性的靶DNA的重组酶 - 聚合酶扩增（RPA）的相关新方法，以允许DNA聚合酶反应的序列特异性引发的单链同源DNA侵入双链DNA。 所公开的方法具有不需要热循环或嗜热酶的优点。 此外，所公开的方法的改进的持续性可以允许将DNA扩增长达数百个百兆位。
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