公开(公告)号：US20230112565A1

公开(公告)日：2023-04-13

申请号：US17799230

申请日：2021-02-12

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Nongjian TAO ,  Shaopeng WANG ,  Pengfei ZHANG

IPC: G01N33/68 ,  B01L3/00 ,  G01N33/543 ,  G01N15/14

Abstract: Detecting single molecules includes binding the single molecules to a surface of an optically transparent substrate, irradiating the surface of the substrate with light having an incident angle selected to achieve total reflection of the light, thereby scattering light from the surface and from the single molecules bound to the surface, and collecting light scatted by the surface and by the single molecules bound to the surface to form a series of images. Systems for detecting single molecules include an optically transparent substrate, a means for flowing a sample solution over a surface of the substrate, a light source configured to irradiate the surface, a camera, and a collection optical system configured to collect light scatted by the surface and by the target molecules on the surface to form a series of images on the camera.

公开(公告)号：US20250012697A1

公开(公告)日：2025-01-09

申请号：US18055033

申请日：2022-11-14

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Fenni ZHANG

IPC: G01N15/0205 ,  G01N15/01 ,  G01N15/0227 ,  G06V10/774 ,  G06V20/69 ,  G16H10/40

Abstract: Provided herein is a method of detecting microorganisms in liquid samples using image features, such as time profiles of object light scattering intensity and time profiles of object position. Related methods, devices, systems, and other aspects are also provided.

公开(公告)号：US20230384311A1

公开(公告)日：2023-11-30

申请号：US18319157

申请日：2023-05-17

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Guangzhong MA

IPC: G01N33/573 ,  G01N33/543

CPC classification number: G01N33/573 ,  G01N2333/912 ,  G01N33/54386

Abstract: Provided herein are methods of detecting unlabeled biomolecules. In some embodiments, the methods include disrupting a cell population sufficient to release unlabeled biomolecules from the cell population to produce released biomolecules in which the cell population is disposed on a first inner surface of a chamber that is disposed substantially within a fluidic device and in which the chamber comprises a fluidic material. In some embodiments, the methods also include binding the released biomolecules to a second inner surface of the chamber to produce surface-bound biomolecules, introducing an incident light toward the second inner surface of the chamber concurrent with, and/or after, producing the surface-bound biomolecules, and detecting light scattered by the surface-bound biomolecules over a duration to produce a set of biomolecule imaging data. Related fluidic devices, systems, and computer readable media are also provided.

公开(公告)号：US20230296635A1

公开(公告)日：2023-09-21

申请号：US18185808

申请日：2023-03-17

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Zijian WAN ,  Heng ZHU

IPC: G01N33/94 ,  G01N33/543 ,  G01N21/64

CPC classification number: G01N33/94 ,  G01N33/54373 ,  G01N21/6428 ,  G01N2333/035 ,  G01N2500/04 ,  G01N2333/726 ,  G01N2021/6439

Abstract: Provided herein are methods of determining binding kinetics of a ligand. In some embodiments, the methods include contacting the ligand with a first surface of a substrate, which first surface comprises an electrically conductive coating and a population of virions connected to the first surface via one or more linker moieties, wherein viral envelopes of the virions display one or more proteins that bind, or are capable of binding, to the ligand, applying an alternating current electric field to the substrate to induce the virions to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the virions over a duration. Related virion oscillator array devices, systems and computer readable media are also provided.

公开(公告)号：US20230230249A1

公开(公告)日：2023-07-20

申请号：US18002438

申请日：2021-06-23

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Fenni ZHANG ,  Nongjian TAO

IPC: G06T7/00 ,  C12Q1/06 ,  C12Q1/18 ,  C12M1/00

CPC classification number: G06T7/0016 ,  C12Q1/06 ,  C12Q1/18 ,  C12M41/06

Abstract: Detecting single bacterial cells in a sample includes collecting, from a sample provided to an imaging apparatus, a multiplicity of images of the sample over a length of time; assessing a trajectory of each bacterial cell in the sample; and assessing, based on the trajectory of each bacterial cell in the sample, a number of bacterial cell divisions that occur in the sample during the length of time.