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公开(公告)号：US20050194250A1
公开(公告)日：2005-09-08
申请号：US11002736
申请日：2004-12-02
CPC分类号： C12M1/00 , C12Q1/6837 , G01N1/00 , G01N27/3276 , C12Q2565/607
摘要： Sensor arrangement, set up as an integrated circuit, having a substrate, at least three sensor electrodes arranged on the substrate such that, in an operating state in which an electrically conductive substance is introduced into the sensor arrangement, the sensor electrodes are coupled to one another by means of the electrically conductive substance, capture molecules immobilized on at least a portion of the sensor electrodes, wherein molecules to be detected can hybridize with the capture molecules, a control circuit for applying a first electrical signal to a selected sensor electrode and simultaneously applying a second electrical signal to at least two of the other sensor electrodes, the first electrical signal being a first temporally variable electrical signal and/or the second electrical signal being a second temporally variable electrical signal, a detection device, which is set up such that, in a first operating state, in which a reference liquid is introduced into the sensor arrangement, a reference value of a third temporally variable electrical signal resulting from the first temporally variable electrical signal and/or from the second temporally variable electrical signal is detected at the selected sensor electrode, and in a second operating state, in which an analyte possibly having molecules to be detected is introduced into the sensor arrangement, a sensor value of the third temporally variable electrical signal resulting from the first temporally variable electrical signal and/or from the second temporally variable electrical signal is detected at the selected sensor electrode, and an evaluation circuit, which, on the basis of the reference value and the sensor value, determines whether a hybridization event has taken place at the selected sensor electrode.
摘要翻译： 设置为集成电路的传感器布置，具有衬底，布置在衬底上的至少三个传感器电极，使得在将导电物质引入传感器装置的操作状态下，传感器电极耦合到一个 另一个通过导电物质捕获固定在传感器电极的至少一部分上的分子，其中待检测的分子可与捕获分子杂交;控制电路，用于将第一电信号施加到所选择的传感器电极，同时 将第二电信号施加到至少两个其它传感器电极，所述第一电信号是第一时间可变电信号和/或所述第二电信号是第二时间可变电信号，所述检测装置被设置为 在第一操作状态下，将参考液体引入到传感器中 在所选择的传感器电极处检测由第一时间可变电信号和/或来自第二时间可变电信号产生的第三时间可变电信号的参考值，并且在第二操作状态中，分析物可能 将要检测的分子引入传感器装置中，在所选择的传感器电极处检测由第一时间可变电信号和/或来自第二时间可变电信号产生的第三时间可变电信号的传感器值， 评估电路，其基于参考值和传感器值确定在所选择的传感器电极处是否发生杂交事件。
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公开(公告)号：US20080036444A1
公开(公告)日：2008-02-14
申请号：US11663078
申请日：2005-09-01
CPC分类号： G01N27/3276
摘要： A sensor arrangement including a control circuit is disclosed. In at least one embodiment, at least one sensor electrode can be charged and/or discharged therewith and a comparator unit for the comparison of a provided voltage for the at least one electrode with a reference voltage. A duration necessary for the charging/discharging of the at least one sensor electrode is determined, whereby, from the determined duration, it is determined whether a sensor event, in the form of a hybridisation between trap molecules and the particles for recording, has occurred.
摘要翻译： 公开了一种包括控制电路的传感器装置。 在至少一个实施例中，可以对至少一个传感器电极进行充电和/或放电，以及用于将所述至少一个电极的提供的电压与参考电压进行比较的比较器单元。 确定至少一个传感器电极的充电/放电所需的持续时间，由此，从所确定的持续时间确定是否发生了陷阱分子和用于记录的颗粒之间的杂交形式的传感器事件 。
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13.发明申请Capacitative biosensor element and method for detecting hybridization events 审中-公开用于检测杂交事件的电容式生物传感器元件和方法公开(公告)号：US20060226030A1
公开(公告)日：2006-10-12
申请号：US10562040
申请日：2004-05-11
IPC分类号： G01F1/64 , G01N33/487
CPC分类号： G01N33/54346 , B01L3/5027 , C12Q1/6825 , C12Q1/6834 , G01N27/3276 , G01N33/5438
摘要： A sensor element is for detecting DNA single strands which are possibly contained in an analyte. The sensor element includes a substrate and at least two electrodes in and/or on the substrate. In a surface area of the substrate, catcher molecules are immobilized and are adapted to hybridize to DNA single strands that are possibly contained in an analyte. The DNA single strands include a label that has dielectric properties that are different from those of the analyte. The electrodes are coupled to a detection device for detecting a change in the capacitative portion of the impedance between the electrodes due to a label that is present in an area surrounding the electrodes as a result of the hybridization event.
摘要翻译： 传感器元件用于检测可能包含在分析物中的DNA单链。 传感器元件包括衬底和衬底中和/或上的至少两个电极。 在底物的表面区域中，捕获剂分子被固定并适于与可能包含在分析物中的DNA单链杂交。 DNA单链包括具有不同于分析物的介电性质的标记。 电极耦合到检测装置，用于由于杂交事件的结果，由于存在于电极周围的区域中的标签，用于检测电极之间的阻抗的电容部分的变化。
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公开(公告)号：US20060216813A1
公开(公告)日：2006-09-28
申请号：US10539817
申请日：2003-12-15
申请人： Walter Gumbrecht , Konrad Mund , Meinrad Schienle , Roland Thewes
发明人： Walter Gumbrecht , Konrad Mund , Meinrad Schienle , Roland Thewes
IPC分类号： C12M1/34
CPC分类号： G01N33/5438 , C12Q1/6825
摘要： A DNA chip includes a carrier and a microarray of spots containing immobilised catcher molecules which are arranged on the carrier. Each spot contains a microelectrode system for the impedance spectroscopic detection of binding events occurring between the catcher molecules and target molecules of an analyte solution applied to the spots. The microelectrode system has a pair of polarisation electrodes in order to produce an alternating electromagnetic field and a pair of sensor electrodes for measuring a voltage drop in the analyte.
摘要翻译： DNA芯片包括布置在载体上的载体和含有固定捕获剂分子的斑点的微阵列。 每个斑点包含用于阻抗光谱检测在施加到斑点的分析物溶液的捕获剂分子和靶分子之间发生的结合事件的微电极系统。 微电极系统具有一对极化电极以产生交变电磁场和一对传感器电极，用于测量分析物中的电压降。
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公开(公告)号：US08262875B2
公开(公告)日：2012-09-11
申请号：US11663078
申请日：2005-09-01
IPC分类号： B01J8/20
CPC分类号： G01N27/3276
摘要： A sensor arrangement including a control circuit is disclosed. In at least one embodiment, at least one sensor electrode can be charged and/or discharged therewith and a comparator unit for the comparison of a provided voltage for the at least one electrode with a reference voltage. A duration necessary for the charging/discharging of the at least one sensor electrode is determined, whereby, from the determined duration, it is determined whether a sensor event, in the form of a hybridization between trap molecules and the particles for recording, has occurred.
摘要翻译： 公开了一种包括控制电路的传感器装置。 在至少一个实施例中，可以对至少一个传感器电极进行充电和/或放电，以及用于将所述至少一个电极的提供的电压与参考电压进行比较的比较器单元。 确定至少一个传感器电极的充电/放电所需的持续时间，由此从确定的持续时间确定是否发生陷阱分子和用于记录的颗粒之间的杂交形式的传感器事件 。
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公开(公告)号：US07572624B2
公开(公告)日：2009-08-11
申请号：US10539817
申请日：2003-12-15
申请人： Walter Gumbrecht , Konrad Mund , Meinrad Schienle , Roland Thewes
发明人： Walter Gumbrecht , Konrad Mund , Meinrad Schienle , Roland Thewes
CPC分类号： G01N33/5438 , C12Q1/6825
摘要： A DNA chip includes a carrier and a microarray of spots containing immobilised catcher molecules which are arranged on the carrier. Each spot contains a microelectrode system for the impedance spectroscopic detection of binding events occurring between the catcher molecules and target molecules of an analyte solution applied to the spots. The microelectrode system has a pair of polarisation electrodes in order to produce an alternating electromagnetic field and a pair of sensor electrodes for measuring a voltage drop in the analyte.
摘要翻译： DNA芯片包括布置在载体上的载体和含有固定捕获剂分子的斑点的微阵列。 每个斑点包含用于阻抗光谱检测在施加到斑点的分析物溶液的捕获剂分子和靶分子之间发生的结合事件的微电极系统。 微电极系统具有一对极化电极以产生交变电磁场和一对传感器电极，用于测量分析物中的电压降。
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17.发明申请公开(公告)号：US20120108456A1
公开(公告)日：2012-05-03
申请号：US13262578
申请日：2010-03-02
CPC分类号： G01N27/3277 , G01N33/5438
摘要： A device for detecting chemical or biochemical substances in fluids includes a first carrier having a sensor array with a plurality of electrochemical sensors. A second carrier includes a porous layer having at least one functional region, in which specifically binding capturing molecules are immobilized. The at least one functional region is arranged directly adjacent to at least one non-functionalized region. Assigned to the at least one functional region and the at least one non-functionalized region are several sensors of the sensor array, for use as an electrochemical camera.
摘要翻译： 用于检测流体中的化学或生化物质的装置包括具有传感器阵列的多个电化学传感器的第一载体。 第二载体包括具有至少一个功能区的多孔层，其中固定有特异性结合的捕获分子。 所述至少一个功能区域直接邻近至少一个非功能化区域布置。 分配给至少一个功能区域和至少一个非功能化区域是传感器阵列的几个传感器，用作电化学照相机。
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18.发明申请公开(公告)号：US20120252055A1
公开(公告)日：2012-10-04
申请号：US13499376
申请日：2010-09-29
申请人： Alexander Frey , Walter Gumbrecht , Peter Paulicka , Meinrad Schienle , Daniel Sickert , Manfred Stanzel
发明人： Alexander Frey , Walter Gumbrecht , Peter Paulicka , Meinrad Schienle , Daniel Sickert , Manfred Stanzel
CPC分类号： G01N27/416 , C12M41/38 , C12M41/46 , G01N27/404 , G01N27/423 , G01N33/48735
摘要： An arrangement and a method measures cell vitalities with a sensor array. The sensor array is formed on a surface of a semiconductor chip. The semiconductor chip has integrated circuits and an integrated circuit is associated with each sensor of the sensor array, for processing the measurement signals of the respective sensor. The integrated circuits are formed in the semiconductor chip spatially in each case below the associated sensor and neighboring sensors of the sensor array have a centre-to-centre in the range of micrometers. The pH and/or pO2 can be measured in the environment of a living cell.
摘要翻译： 一种安排和方法用传感器阵列测量细胞活力。 传感器阵列形成在半导体芯片的表面上。 半导体芯片具有集成电路，并且集成电路与传感器阵列的每个传感器相关联，用于处理相应传感器的测量信号。 集成电路在空间上形成在半导体芯片中，在每个情况下在相关传感器下方，并且传感器阵列的相邻传感器具有在微米范围内的中心到中心。 pH和/或pO2可以在活细胞的环境中测量。
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公开(公告)号：US09995703B2
公开(公告)日：2018-06-12
申请号：US13262578
申请日：2010-03-02
IPC分类号： C40B60/00 , C40B60/12 , G01N27/327 , G01N33/543 , C40B30/04 , C40B50/18
CPC分类号： G01N27/3277 , G01N33/5438
摘要： A device for detecting chemical or biochemical substances in fluids for use in an electrochemical camera. The device includes a first carrier having a sensor array with a plurality of electrochemical sensors. A second carrier includes a porous layer having at least one functional region, in which specifically binding capturing molecules are immobilized. The at least one functional region is arranged directly adjacent to at least one non-functionalized region. Assigned to the at least one functional region and the at least one non-functionalized region are several sensors of the sensor array, for use as the electrochemical camera.
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20.发明授权公开(公告)号：US08916035B2
公开(公告)日：2014-12-23
申请号：US13499376
申请日：2010-09-29
申请人： Alexander Frey , Walter Gumbrecht , Peter Paulicka , Meinrad Schienle , Daniel Sickert , Manfred Stanzel
发明人： Alexander Frey , Walter Gumbrecht , Peter Paulicka , Meinrad Schienle , Daniel Sickert , Manfred Stanzel
IPC分类号： G01N21/41 , C12M1/34 , G01N27/42 , G01N27/416 , G01N27/404
CPC分类号： G01N27/416 , C12M41/38 , C12M41/46 , G01N27/404 , G01N27/423 , G01N33/48735
摘要： An arrangement and a method measures cell vitalities with a sensor array. The sensor array is formed on a surface of a semiconductor chip. The semiconductor chip has integrated circuits and an integrated circuit is associated with each sensor of the sensor array, for processing the measurement signals of the respective sensor. The integrated circuits are formed in the semiconductor chip spatially in each case below the associated sensor and neighboring sensors of the sensor array have a center-to-center in the range of micrometers. The pH and/or pO2 can be measured in the environment of a living cell.
摘要翻译： 一种安排和方法用传感器阵列测量细胞活力。 传感器阵列形成在半导体芯片的表面上。 半导体芯片具有集成电路，并且集成电路与传感器阵列的每个传感器相关联，用于处理相应传感器的测量信号。 集成电路在空间上形成在半导体芯片中，在每个情况下在相关传感器下方，并且传感器阵列的相邻传感器具有在微米范围内的中心到中心。 pH和/或pO2可以在活细胞的环境中测量。
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