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41 条记录 (耗时 0.032 秒)

    Method for detecting reactions by means of coincidence analysis
    12.
    发明授权
    Method for detecting reactions by means of coincidence analysis 有权
    通过重合分析检测反应的方法

    公开(公告)号：US06200818B1

    公开(公告)日：2001-03-13

    申请号：US09581217

    申请日：2000-06-22

    申请人： Manfred Eigen ,  Thorsten Winkler ,  Jens Stephan ,  Petra Schwille ,  Andre Koltermann ,  Ulrich Kettling ,  Klaus Dörre ,  Jan Bieschke

    发明人： Manfred Eigen ,  Thorsten Winkler ,  Jens Stephan ,  Petra Schwille ,  Andre Koltermann ,  Ulrich Kettling ,  Klaus Dörre ,  Jan Bieschke

    IPC分类号： G01N2164

    CPC分类号： G01N21/6408 ,  G01N2021/6421

    摘要： The invention relates to a method for detecting reactions and conformational changes of analytes in a sample by coincidence analysis, as follows: the sample is marked with at least two different fluorescent dyes and then illuminated with at least one laser in order to stimulate the emission of fluorescence; the fluorescence signals are detected by at least two detection units and each signal is broken down into any simultaneous time segments with the desired time slot widths; the number of signals contained in at least one time segment and/or the time intervals between signals in the time segments are detected; a coincidence analysis of the data detected is carried out for at least one time segment of the first detection unit with at least one isochronous time segment of the second detection unit; statistics are produced showing the results of the coincidence analysis and/or the results are subjected to a threshold value analysis, and these statistics or a combination of more statistics are evaluated for the presence of characteristic features.

    摘要翻译： 本发明涉及通过重合分析检测样品中分析物的反应和构象变化的方法，如下：样品用至少两种不同的荧光染料标记，然后用至少一种激光照射，以刺激样品的发射 荧光; 荧光信号由至少两个检测单元检测，并且每个信号被分解成具有所需时隙宽度的任何同步时间段; 检测包含在至少一个时间段中的信号数量和/或时间段中的信号之间的时间间隔; 对于具有第二检测单元的至少一个等时时间段的第一检测单元的至少一个时间段执行检测到的数据的一致性分析; 产生显示符合分析的结果和/或对结果进行阈值分析的统计量，并且对特征特征的存在来评估这些统计量或更多统计量的组合。

    Generation of Chemical Building Blocks from Plant Biomass by Selective Depolymerization
    16.
    发明申请
    Generation of Chemical Building Blocks from Plant Biomass by Selective Depolymerization 有权
    通过选择性解聚从植物生物量生成化学构件

    公开(公告)号：US20090004697A1

    公开(公告)日：2009-01-01

    申请号：US12138096

    申请日：2008-06-12

    申请人： Andre Koltermann ,  Ulrich Kettling ,  Thomas Bruck ,  Markus Rarbach

    发明人： Andre Koltermann ,  Ulrich Kettling ,  Thomas Bruck ,  Markus Rarbach

    IPC分类号： C12P19/00

    CPC分类号： C12N9/2434 ,  C12N9/0061 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14 ,  C12P19/16 ,  C12P19/18 ,  C12P19/20 ,  C12P19/22 ,  C12P19/24 ,  C13B35/005 ,  C13K1/02 ,  C13K1/06 ,  C13K13/002

    摘要： The invention concerns a method for the enzymatic treatment of raw polymeric feedstock comprising the following steps: (a) preferably separation of soluble components from the raw polymeric feedstock, (b) treating the raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock; and (c) separating the defined monomeric or oligomeric building blocks produced in step b) from the remainder of the raw polymeric feedstock. Preferably, the enzyme system used in step b) contains not more than 50%, preferably not more than 20%, more preferably not more than 10%, more preferably not more than 5%, more preferably not more than 2%, more preferably not more than 1% of other enzyme activities apart from the enzyme activity resulting in liberation of said defined monomeric or oligomeric building blocks from the raw polymeric feedstock according to step b). Further aspects of the invention concern the use of “less pure” and thus less costly enzyme systems in subsequent enzymatic treatment steps and methods for determining the optimum sequence of enzymatic treatment steps by analysis of the raw polymeric feedstock used.

    摘要翻译： 本发明涉及用于酶处理原料聚合物原料的方法，包括以下步骤：（a）优选将可溶性组分与原料聚合原料分离，（b）用酶体系处理原料聚合物原料，以释放限定的可溶性 来自不溶性原料聚合原料的单体或低聚结构单元; 和（c）将步骤b）中制备的限定的单体或低聚结构单元与原始聚合原料的其余部分分离。 优选地，步骤b）中使用的酶系统含有不超过50％，优选不超过20％，更优选不超过10％，更优选不超过5％，更优选不超过2％ 不超过1％的其它酶活性，除了酶活性，导致根据步骤b）从所述原料聚合物原料释放所述限定的单体或低聚结构单元。 本发明的另外方面涉及在随后的酶处理步骤中使用“较不纯”且因此较低成本的酶体系，以及通过分析所用原料聚合物原料来确定酶处理步骤的最佳顺序的方法。

    Method for the production of biopolymers with modified properties
    18.
    发明授权
    Method for the production of biopolymers with modified properties 有权
    生产具有改性特性的生物聚合物的方法

    公开(公告)号：US06821758B1

    公开(公告)日：2004-11-23

    申请号：US09708497

    申请日：2000-11-09

    申请人： Andre Koltermann ,  Ulrich Kettling ,  Manfred Eigen

    发明人： Andre Koltermann ,  Ulrich Kettling ,  Manfred Eigen

    IPC分类号： C12P1934

    CPC分类号： C12N15/1027

    摘要： A method for the production of biopolymers with modified properties wherein at least one cycle comprising the following steps is completed: (a) providing a population of single-stranded polynucleotide molecules, wherein individual polynucleotide molecules comprise homologous and heterologous sequence segments and wherein individual ones of said single-stranded polynucleotide molecules can form double-stranded polynucleotide molecules with other ones of said single-stranded polynucleotide molecules within said population; (b) forming double-stranded polynucleotide molecules from the population of single-stranded polynucleotide molecules provided according to step (a) comprising double-stranded polynucleotide molecules with different heterologous sequence segments; (c) partially and exonucleolytically degrading the single-strands of the double-stranded polynucleotide molecules produced according to step (b); and (d) temple-directed single-stand synthesizing the degraded ends of the partially degraded double strand produced according to step (c), wherein steps (c) and (d) may be carried out sequentially or contemporaneously.

    摘要翻译： 一种用于生产具有改进性质的生物聚合物的方法，其中完成包括以下步骤的至少一个循环：（a）提供一群单链多核苷酸分子，其中单个多核苷酸分子包含同源和异源序列区段， 所述单链多核苷酸分子可以与所述群体内的所述单链多核苷酸分子中的其它单链多核苷酸分子形成双链多核苷酸分子;（b）从根据步骤（I）提供的单链多核苷酸分子群体形成双链多核苷酸分子 a）包含具有不同异源序列片段的双链多核苷酸分子;（c）部分和外核解离降解根据步骤（b）制备的双链多核苷酸分子的单链; 和（d）合成根据步骤（c）制备的部分降解的双链的降解末端的神经导向单支，其中步骤（c）和（d）可以顺序或同时进行。