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    METHOD FOR THE TREATMENT OF A SAMPLE CONTAINING BIOMOLECULES
    11.
    发明申请
    METHOD FOR THE TREATMENT OF A SAMPLE CONTAINING BIOMOLECULES 有权
    用于处理含有生物分子样品的方法

    公开(公告)号：US20100323363A1

    公开(公告)日：2010-12-23

    申请号：US12862156

    申请日：2010-08-24

    申请人： Christian KORFHAGE ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    发明人： Christian KORFHAGE ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    IPC分类号： C12Q1/68

    CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

    摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡聚物和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括均匀的杂多聚物，d）R1R2NR3型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）碳酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

    Method for the treatment of a sample containing biomolecules
    12.
    发明授权
    Method for the treatment of a sample containing biomolecules 有权
    用于处理含有生物分子的样品的方法

    公开(公告)号：US07838233B2

    公开(公告)日：2010-11-23

    申请号：US11910247

    申请日：2006-04-01

    申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    IPC分类号： C12Q1/68 ,  C12P21/06 ,  C07K1/00 ,  C07K14/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-older heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides In particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

    摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡聚物和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括同质杂多酸，d）R1R2NR3类型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5 - 烷基和芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供一种用于裂解生物样品的方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

    Method For Facilitating an Automated Isolation of a Biopolymer Using Magnetic Particles
    13.
    发明申请
    Method For Facilitating an Automated Isolation of a Biopolymer Using Magnetic Particles 有权
    促进使用磁性颗粒自动分离生物聚合物的方法

    公开(公告)号：US20080132694A1

    公开(公告)日：2008-06-05

    申请号：US10567351

    申请日：2004-08-16

    申请人： Ralf Himmelreich ,  Sabine Werner

    发明人： Ralf Himmelreich ,  Sabine Werner

    IPC分类号： C07H21/04

    CPC分类号： C12N15/1013

    摘要： The present invention provides a method for isolating biopolymers, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from an aqueous solution utilizing magnetic particles, particularly silica magnetic particles. The method of the present invention involves forming a complex of the magnetic particles and the biopolymer in a mixture of said particles and said aqueous solution, comprising a salt and an additive; separating the complex from the mixture by a magnetic force, and eluting the biopolymer from the complex, wherein advantageously no substantial clustering of said magnetic particles occurs during the performance of the method. In a preferred embodiment the method of the invention is performed as an automated process.

    摘要翻译： 本发明提供一种利用磁性颗粒，特别是二氧化硅磁性颗粒从水溶液中分离生物聚合物，特别是核酸，如DNA或RNA或DNA和RNA的混合分子的方法。 本发明的方法包括在所述颗粒和所述水溶液的混合物中形成磁性颗粒和生物聚合物的复合物，其包含盐和添加剂; 通过磁力将复合物与混合物分离，并从复合物中洗脱生物聚合物，其中在实施该方法期间有利地不发生所述磁性颗粒的基本聚集。 在优选实施例中，本发明的方法作为自动化过程进行。

    Helicase dependent isothermal amplification using nicking enzymes
    14.
    发明授权
    Helicase dependent isothermal amplification using nicking enzymes 有权
    使用切酶的Helicase依赖性等温扩增

    公开(公告)号：US09005933B2

    公开(公告)日：2015-04-14

    申请号：US13817174

    申请日：2011-08-16

    申请人： Christian Korfhage ,  Gerd Grosshauser ,  Thomas Rothmann ,  Ralf Himmelreich

    发明人： Christian Korfhage ,  Gerd Grosshauser ,  Thomas Rothmann ,  Ralf Himmelreich

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q2521/301 ,  C12Q2521/513

    摘要： The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.

    摘要翻译： 本发明涉及用于扩增模板核酸的方法，其中所述方法包括在切口内切核酸酶存在下使用解旋酶依赖性扩增（HDA）反应扩增所述模板核酸，并且其中所述模板核酸包含识别的序列 通过所述切口内切核酸酶或由所述切口内切核酸酶识别的序列在HDA反应期间引入模板核酸。 本发明还涉及用于扩增核酸的试剂盒，其包含切口内切核酸酶，解旋酶和DNA聚合酶。

    DETECTION OF MULTIPLE NUCLEIC ACID SEQUENCES IN A REACTION CARTRIDGE
    17.
    发明申请
    DETECTION OF MULTIPLE NUCLEIC ACID SEQUENCES IN A REACTION CARTRIDGE 有权
    在反应盒中检测多个核酸序列

    公开(公告)号：US20120107818A1

    公开(公告)日：2012-05-03

    申请号：US13318424

    申请日：2010-05-04

    申请人： Thomas Rothmann ,  Holger Engel ,  Ralf Himmelreich ,  Andy Wendy ,  Rainer Dahlke

    发明人： Thomas Rothmann ,  Holger Engel ,  Ralf Himmelreich ,  Andy Wendy ,  Rainer Dahlke

    IPC分类号： C12Q1/68 ,  C12M1/40

    CPC分类号： C12Q1/6818 ,  Y02A50/59 ,  C12Q2527/107 ,  C12Q2537/143 ,  C12Q2565/629

    摘要： The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising the following steps, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set, wherein (a) each probe set consists of at least three probes, (b) each of the probes is specific for a nucleic acid sequence, (c) there are at least two probes in each set which carry an identical label, (d) each of the probes in a given probe set that carries an identical label has a melting temperature (Tm) which differs by more than 2° C. from the other probe in said probe set with the same label, (e) wherein the probes carrying the identical label differ in melting temperature (Tm) in a way that they are distinguishable by melting point, (f) performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.

    摘要翻译： 本发明涉及用于扩增和检测反应盒中的核酸序列的方法，包括以下步骤：（i）提供包含至少一种核酸分子的样品，（ii）在所述盒的第一反应室中提供试剂 用于扩增反应，（iii）将样品与扩增试剂混合，（iv）扩增筒的第一反应室中的至少一种核酸，（v）将至少部分扩增反应转移到第二和 每个探针组由至少三个探针组成，（b）每个探针对于核酸序列是特异性的，（c）存在至少两个探针 在每个携带相同标签的组中，（d）给定探针组中携带相同标记物的每个探针具有与所述探针中的另一个探针相差大于2℃的熔解温度（Tm） 设置相同的标签，（e）其中携带相同标记的探针以熔点可区分的方式在熔融温度（Tm）上不同，（f）进行熔点分析以确定哪个探针 已经特异地结合核酸。

    Method for enriching short-chain nucleic acids
    19.
    发明授权
    Method for enriching short-chain nucleic acids 有权
    丰富短链核酸的方法

    公开(公告)号：US07977109B2

    公开(公告)日：2011-07-12

    申请号：US12096155

    申请日：2006-12-08

    申请人： Christoph Ritt ,  Ralf Himmelreich ,  Martin Weber

    发明人： Christoph Ritt ,  Ralf Himmelreich ,  Martin Weber

    IPC分类号： G01N30/00

    CPC分类号： C12N15/101 ,  Y10T436/143333 ,  Y10T436/255

    摘要： The present invention relates to a method for enriching nucleic acids with a length of not more than 300 nucleotides. The invention also relates to a kit for enriching nucleic acids with a length of not more than 300 nucleotides, to the use of such a kit, to the use of an anion exchange matrix and to a method for treating a disease.

    摘要翻译： 本发明涉及一种富集长度不超过300个核苷酸的核酸的方法。 本发明还涉及用于富集长度不超过300个核苷酸的核酸的试剂盒，使用这种试剂盒，使用阴离子交换基质和治疗疾病的方法。