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公开(公告)号：US20130210019A1
公开(公告)日：2013-08-15
申请号：US13817174
申请日：2011-08-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2521/301 , C12Q2521/513
摘要： The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.
摘要翻译： 本发明涉及用于扩增模板核酸的方法，其中所述方法包括在切口内切核酸酶存在下使用解旋酶依赖性扩增（HDA）反应扩增所述模板核酸，并且其中所述模板核酸包含识别的序列 通过所述切口内切核酸酶或由所述切口内切核酸酶识别的序列在HDA反应期间引入模板核酸。 本发明还涉及用于扩增核酸的试剂盒，其包含切口内切核酸酶，解旋酶和DNA聚合酶。
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公开(公告)号：US09005933B2
公开(公告)日：2015-04-14
申请号：US13817174
申请日：2011-08-16
CPC分类号： C12Q1/6844 , C12Q2521/301 , C12Q2521/513
摘要： The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.
摘要翻译： 本发明涉及用于扩增模板核酸的方法，其中所述方法包括在切口内切核酸酶存在下使用解旋酶依赖性扩增（HDA）反应扩增所述模板核酸，并且其中所述模板核酸包含识别的序列 通过所述切口内切核酸酶或由所述切口内切核酸酶识别的序列在HDA反应期间引入模板核酸。 本发明还涉及用于扩增核酸的试剂盒，其包含切口内切核酸酶，解旋酶和DNA聚合酶。
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公开(公告)号：US20120149011A1
公开(公告)日：2012-06-14
申请号：US13390887
申请日：2010-08-16
CPC分类号： C12Q1/6844 , C12Q2525/155 , C12Q2525/15 , C12Q2525/131
摘要： The invention relates to a method for detecting target nucleic acids which are detected by means of a specific sequence tag which is not part of the target nucleic acid.
摘要翻译： 本发明涉及通过不属于靶核酸的特异性序列标签检测靶核酸的方法。
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4.发明授权公开(公告)号：US09574225B2
公开(公告)日：2017-02-21
申请号：US13141593
申请日：2009-12-23
CPC分类号： C12Q1/6806 , C12N15/1013 , C12Q1/686 , C12Q2565/629 , C12Q2563/143 , C12Q2549/119 , C12Q2547/101
摘要： The invention relates to the preparation of a biological sample for performing verifications and examinations, wherein the aim of the invention is the creation of a method for preparing a biological sample having an improved PCR sensitivity compared to the reference standard having standard PCR without having to raise the cost thereof.
摘要翻译： 本发明涉及用于进行验证和检查的生物样品的制备，其中本发明的目的是创建与具有标准PCR的参考标准相比具有改进的PCR灵敏度的生物样品的制备方法，而不必提高 其成本。
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公开(公告)号：US08937174B2
公开(公告)日：2015-01-20
申请号：US12864641
申请日：2009-01-13
申请人： Thomas Rothmann , Ralf Himmelreich , Thomas Laue
发明人： Thomas Rothmann , Ralf Himmelreich , Thomas Laue
CPC分类号： B01L3/502715 , B01J2219/00335 , B01J2219/00373 , B01J2219/00423 , B01J2219/00605 , B01J2219/00641 , B01J2219/00722 , B01L7/52 , B01L2200/025 , B01L2200/027 , B01L2200/10 , B01L2300/0672 , B01L2300/0681 , B01L2300/0816 , B01L2300/1827 , C12M47/06
摘要： The invention relates to a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further processed by a microfluidic system. A container is provided with a filter and can be or is closed. A biological sample is introduced into the container. An inlet or outlet for liquids is mounted downstream of the filter. In order to carry out processing, liquids that are present in the container are not only sucked off through the filter and passed on via the inlet or outlet, but liquids required for processing are also pumped into the container through the filter. The container can be connected to a microfluidic system in a relatively easy manner since only one conduit is required for an automated processing.
摘要翻译： 本发明涉及一种用于自动处理生物样品的方法和装置，其可以具有相对较大的体积并且可以由微流体系统进一步处理。 容器设有过滤器，可以被关闭。 将生物样品引入容器中。 用于液体的入口或出口安装在过滤器的下游。 为了进行处理，存在于容器中的液体不仅通过过滤器吸出并经由入口或出口通过，而且处理所需的液体也通过过滤器被泵送到容器中。 容器可以以相对容易的方式连接到微流体系统，因为仅需要一个管道用于自动化处理。
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公开(公告)号：US20120107818A1
公开(公告)日：2012-05-03
申请号：US13318424
申请日：2010-05-04
申请人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wendy , Rainer Dahlke
发明人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wendy , Rainer Dahlke
CPC分类号： C12Q1/6818 , Y02A50/59 , C12Q2527/107 , C12Q2537/143 , C12Q2565/629
摘要： The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising the following steps, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set, wherein (a) each probe set consists of at least three probes, (b) each of the probes is specific for a nucleic acid sequence, (c) there are at least two probes in each set which carry an identical label, (d) each of the probes in a given probe set that carries an identical label has a melting temperature (Tm) which differs by more than 2° C. from the other probe in said probe set with the same label, (e) wherein the probes carrying the identical label differ in melting temperature (Tm) in a way that they are distinguishable by melting point, (f) performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.
摘要翻译： 本发明涉及用于扩增和检测反应盒中的核酸序列的方法，包括以下步骤：（i）提供包含至少一种核酸分子的样品，（ii）在所述盒的第一反应室中提供试剂 用于扩增反应，（iii）将样品与扩增试剂混合，（iv）扩增筒的第一反应室中的至少一种核酸，（v）将至少部分扩增反应转移到第二和 每个探针组由至少三个探针组成，（b）每个探针对于核酸序列是特异性的，（c）存在至少两个探针 在每个携带相同标签的组中，（d）给定探针组中携带相同标记物的每个探针具有与所述探针中的另一个探针相差大于2℃的熔解温度（Tm） 设置相同的标签，（e）其中携带相同标记的探针以熔点可区分的方式在熔融温度（Tm）上不同，（f）进行熔点分析以确定哪个探针 已经特异地结合核酸。
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公开(公告)号：US08852863B2
公开(公告)日：2014-10-07
申请号：US13318424
申请日：2010-05-04
申请人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wende , Rainer Dahlke
发明人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wende , Rainer Dahlke
CPC分类号： C12Q1/6818 , Y02A50/59 , C12Q2527/107 , C12Q2537/143 , C12Q2565/629
摘要： The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.
摘要翻译： 本发明涉及用于扩增和检测反应盒中的核酸序列的方法，其包括：（i）提供包含至少一个核酸分子的样品，（ii）在所述盒的第一反应室中提供用于扩增的试剂 反应，（iii）将样品与扩增试剂混合，（iv）扩增筒的第一反应室中的至少一种核酸，（v）将至少部分扩增反应转移到第二和第三反应室 的每个包含探针组并进行熔点分析，以便确定哪个探针具体结合核酸。
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8.发明申请公开(公告)号：US20110318784A1
公开(公告)日：2011-12-29
申请号：US13141593
申请日：2009-12-23
CPC分类号： C12Q1/6806 , C12N15/1013 , C12Q1/686 , C12Q2565/629 , C12Q2563/143 , C12Q2549/119 , C12Q2547/101
摘要： The invention relates to the preparation of a biological sample for performing verifications and examinations, wherein the aim of the invention is the creation of a method for preparing a biological sample having an improved PCR sensitivity compared to the reference standard having standard PCR without having to raise the cost thereof.
摘要翻译： 本发明涉及用于进行验证和检查的生物样品的制备，其中本发明的目的是创建与具有标准PCR的参考标准相比具有改进的PCR灵敏度的生物样品的制备方法，而不必提高 其成本。
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9.发明授权公开(公告)号：US08361326B2
公开(公告)日：2013-01-29
申请号：US12676376
申请日：2008-09-19
申请人： Ralf Himmelreich , Thomas Rothmann
发明人： Ralf Himmelreich , Thomas Rothmann
CPC分类号： B03C1/286 , B03C1/01 , B03C2201/18
摘要： The present invention relates to a device and a method for treating liquids with magnetic particles, wherein at least one further central element which ensures collection and homogenization of the particles is additionally provided.
摘要翻译： 本发明涉及一种用于用磁性颗粒处理液体的装置和方法，其中还提供了确保颗粒收集和均质化的至少一个另外的中心元件。
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公开(公告)号：US20120070823A1
公开(公告)日：2012-03-22
申请号：US12864641
申请日：2009-01-13
申请人： Thomas Rothmann , Ralf Himmelreich , Thomas Laue
发明人： Thomas Rothmann , Ralf Himmelreich , Thomas Laue
CPC分类号： B01L3/502715 , B01J2219/00335 , B01J2219/00373 , B01J2219/00423 , B01J2219/00605 , B01J2219/00641 , B01J2219/00722 , B01L7/52 , B01L2200/025 , B01L2200/027 , B01L2200/10 , B01L2300/0672 , B01L2300/0681 , B01L2300/0816 , B01L2300/1827 , C12M47/06
摘要： The invention relates to a method and a device for processing a biological sample. According to the invention, the processing comprises binding, washing and eluting biomolecules of the biological sample. The aim of the invention is to provide a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further process by a microfluidic system. According to the invention, a container (1) is provided with a filter (10) and can be or is closed. A biological sample is introduced into the container. An inlet or outlet (4) for liquids is mounted downstream of the filter. In order to carry out processing, liquids that are present in the container are not only sucked off through the filter and passed on via the inlet or outlet, but liquids required for processing are also pumped into the container through the filter. The container can be connected to a microfluidic system in a relatively easy manner since only one conduit is required for an automated processing.
摘要翻译： 本发明涉及一种用于处理生物样品的方法和装置。 根据本发明，加工包括生物样品的结合，洗涤和洗脱生物分子。 本发明的目的是提供一种用于自动处理生物样品的方法和装置，其可以具有相对较大的体积并且可以通过微流体系统进一步处理。 根据本发明，容器（1）设置有过滤器（10）并且可以是或者是关闭的。 将生物样品引入容器中。 用于液体的入口或出口（4）安装在过滤器的下游。 为了进行处理，存在于容器中的液体不仅通过过滤器吸出并经由入口或出口通过，而且处理所需的液体也通过过滤器被泵送到容器中。 容器可以以相对容易的方式连接到微流体系统，因为仅需要一个管道用于自动化处理。
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