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19 条记录 (耗时 0.025 秒)

    Type II restriction endonuclease SexAI
    11.
    发明授权
    Type II restriction endonuclease SexAI 失效
    II型限制性内切酶SexAI

    公开(公告)号：US5354669A

    公开(公告)日：1994-10-11

    申请号：US94374

    申请日：1993-07-19

    申请人： Klaus Kaluza ,  Johannes Auer ,  Bruno Frey

    发明人： Klaus Kaluza ,  Johannes Auer ,  Bruno Frey

    IPC分类号： C12N9/22 ,  C12P19/34

    CPC分类号： C12N9/22

    摘要： The new type II restriction endonuclease SexAI has the following recognition sequence and preferably cleaves at the cleavage site defined by the mark: ##STR1## It is preferably obtainable from microorganisms of the genus Streptomyces.

    摘要翻译： 新型II型限制性内切核酸酶SexAI具有以下识别序列，优选在由标记：链霉菌属的无机物定义的切割位点切割。

    Method for the recombinant expression of an N-terminal fragment of hepatocyte growth factor
    13.
    发明授权
    Method for the recombinant expression of an N-terminal fragment of hepatocyte growth factor 失效
    重组表达肝细胞生长因子N-末端片段的方法

    公开(公告)号：US07754448B2

    公开(公告)日：2010-07-13

    申请号：US10591045

    申请日：2005-03-02

    申请人： Johannes Auer ,  Apollon Papadimitriou ,  Christian Schantz ,  Stefan Seeber

    发明人： Johannes Auer ,  Apollon Papadimitriou ,  Christian Schantz ,  Stefan Seeber

    IPC分类号： C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C07K14/4753 ,  C12N15/70

    摘要： A method for the production of the α-chain of hepatocyte growth factor or an N-terminal fragment thereof (NK polypeptide) by expression of a nucleic acid encoding said NK polypeptide in a microbial host cell, isolating inclusion bodies containing said NK polypeptide in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK polypeptide, which is characterized in that in said nucleic acid at least one of the codons of amino acids selected from the group consisting of codons at positions 33, 35 and 36 is CGT, results in an improved expression yield.

    摘要翻译： 通过在微生物宿主细胞中表达编码所述NK多肽的核酸，通过将包含所述NK多肽的包含体分离成变性来生产肝细胞生长因子或其N-末端片段（NK多肽）的α-链的方法 形式，包涵体的溶解和变性NK多肽的生成，其特征在于在所述核酸中，选自33,35和36位的密码子中的至少一个氨基酸的密码子是CGT， 导致表达产率提高。

    Nucleic acid molecule comprising a nucleic acid sequence coding for a chemokine, a neuropeptide precursor, or at least one neuropeptide
    18.
    发明申请
    Nucleic acid molecule comprising a nucleic acid sequence coding for a chemokine, a neuropeptide precursor, or at least one neuropeptide 审中-公开
    包含编码趋化因子，神经肽前体或至少一种神经肽的核酸序列的核酸分子

    公开(公告)号：US20080319165A1

    公开(公告)日：2008-12-25

    申请号：US12010221

    申请日：2008-01-22

    申请人： Hans Werner Muller ,  Frank Bosse ,  Mark Gleichmann ,  Clemens Gillen ,  Johannes Auer

    发明人： Hans Werner Muller ,  Frank Bosse ,  Mark Gleichmann ,  Clemens Gillen ,  Johannes Auer

    IPC分类号： C07K7/00 ,  C07K14/00

    CPC分类号： C07K14/522 ,  A61K38/00 ,  A61K48/00

    摘要： The invention concerns a nucleic acid molecule which includes a nucleic acid sequence coding for a chemokine, a neuropeptide precursor or at least one neuropeptide, as well as a host cell which contains this nucleic acid molecule. In addition the invention concerns a polypeptide molecule which functions as chemokine or neuropeptide or contains at least one neuropeptide, as well as fragments thereof which include at least one neuropeptide, and a procedure for the manufacture of the polypeptide molecule or of a fragment thereof. In addition the invention concerns antibodies, demonstration procedures and test-kits as well as pharmaceutical preparations.The purpose on which present invention is based is to make new means available which can be put to use aimed at the diagnosis and/or treatment of diseases which are associated with a defect of the SDF-1 factor or its receptor (CXCR4).

    摘要翻译： 本发明涉及核酸分子，其包括编码趋化因子，神经肽前体或至少一种神经肽的核酸序列，以及含有该核酸分子的宿主细胞。 此外，本发明涉及用作趋化因子或神经肽或含有至少一种神经肽的多肽分子及其包含至少一种神经肽的片段，以及用于制备多肽分子或其片段的方法。 此外，本发明涉及抗体，示范程序和测试试剂盒以及药物制剂。 本发明所基于的目的是提供可用于诊断和/或治疗与SDF-1因子或其受体（CXCR4）的缺陷相关的疾病的新手段。

    Method for the recombinant expression of an n-terminal fragment of hepatocyte growth factor
    19.
    发明申请
    Method for the recombinant expression of an n-terminal fragment of hepatocyte growth factor 失效
    重组表达肝细胞生长因子的n末端片段的方法

    公开(公告)号：US20070154985A1

    公开(公告)日：2007-07-05

    申请号：US10591045

    申请日：2005-03-02

    申请人： Johannes Auer ,  Apollon Papadimitriou ,  Christian Schantz ,  Stefan Seeber

    发明人： Johannes Auer ,  Apollon Papadimitriou ,  Christian Schantz ,  Stefan Seeber

    IPC分类号： C07K14/475 ,  C07H21/04 ,  C12P21/06

    CPC分类号： C07K14/4753 ,  C12N15/70

    摘要： A method for the production of a-chain of hepatocyte growth factor or an N-terminal fragment thereof (NK polypeptide) by expression of a nucleic acid encoding said NK polypeptide in a microbial host cell, isolating of inclusion bodies containing said NK polypeptide in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK polypeptide, which is characterized in that in said nucleic acid at least one of the codons of amino acids selected from the group consisting of codons at positions 33, 35 and 36 is CGT, results in an improved expression yield.

    摘要翻译： 通过在微生物宿主细胞中表达编码所述NK多肽的核酸，通过在变性中分离含有所述NK多肽的包涵体来生产肝细胞生长因子或其N-末端片段（NK多肽）的α-链的方法 形式，包涵体的溶解和变性NK多肽的生成，其特征在于，在所述核酸中，选自33,35和36位的密码子的氨基酸的至少一个密码子是CGT， 导致表达产率提高。