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    Parameters for use in particle discrimination
    11.
    发明授权
    Parameters for use in particle discrimination 有权

    公开(公告)号：US11988589B2

    公开(公告)日：2024-05-21

    申请号：US17588040

    申请日：2022-01-28

    Applicant: Becton, Dickinson and Company

    Inventor： Matthew Bahr ,  Eric D. Diebold ,  Jonathan Lin ,  Keegan Owsley

    IPC: G01N15/0227 ,  G01N15/1404

    CPC classification number: G01N15/0227 ,  G01N15/1404

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    Multi-Modal Fluorescence Imaging Flow Cytometry System
    12.
    发明公开
    Multi-Modal Fluorescence Imaging Flow Cytometry System 审中-公开

    公开(公告)号：US20230288311A1

    公开(公告)日：2023-09-14

    申请号：US18198478

    申请日：2023-05-17

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64 ,  G02B21/06

    CPC classification number: G01N15/1434 ,  G01N15/14 ,  G01N15/1425 ,  G01N15/147 ,  G01N15/1475 ,  G01N15/1429 ,  G01N21/645 ,  G02B21/06 ,  G01N2015/1447 ,  G01N2015/1454 ,  G01N2015/1006

    Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

    Systems for cell sorting based on frequency-encoded images and methods of use thereof
    15.
    发明授权
    Systems for cell sorting based on frequency-encoded images and methods of use thereof 有权

    公开(公告)号：US11493427B2

    公开(公告)日：2022-11-08

    申请号：US16817080

    申请日：2020-03-12

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Matthew Bahr ,  Keegan Owsley

    IPC: G06K9/00 ,  G01N15/14 ,  G06T7/66 ,  G06T7/70 ,  G06T7/136 ,  G06T7/11 ,  G01N15/10

    Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.

    Cell sorting using a high throughput fluorescence flow cytometer
    16.
    发明授权
    Cell sorting using a high throughput fluorescence flow cytometer 有权

    公开(公告)号：US11105728B2

    公开(公告)日：2021-08-31

    申请号：US16814394

    申请日：2020-03-10

    Applicant: Becton, Dickinson and Company

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64 ,  G01N15/10

    Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

    Phase-Correction Of Radiofrequency-Multiplexed Signals
    17.
    发明申请
    Phase-Correction Of Radiofrequency-Multiplexed Signals 有权

    公开(公告)号：US20250035535A1

    公开(公告)日：2025-01-30

    申请号：US18817809

    申请日：2024-08-28

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Keegan Owsley ,  Matthew Bahr

    IPC: G01N15/14 ,  G01N15/1404 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/149

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    Multi-Modal Fluorescence Imaging Flow Cytometry System
    20.
    发明公开
    Multi-Modal Fluorescence Imaging Flow Cytometry System 审中-公开

    公开(公告)号：US20230333002A1

    公开(公告)日：2023-10-19

    申请号：US18214317

    申请日：2023-06-26

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64 ,  G02B21/06 ,  G01N15/10

    CPC classification number: G01N15/1434 ,  G01N15/1475 ,  G01N15/1425 ,  G01N15/147 ,  G01N15/1429 ,  G01N21/645 ,  G01N15/14 ,  G02B21/06 ,  G01N2015/1006 ,  G01N2015/1454 ,  G01N2021/6482 ,  G01N2015/1447

    Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

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