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    Digital amplification assays with unconventional and/or inverse changes in photoluminescence
    14.
    发明授权
    Digital amplification assays with unconventional and/or inverse changes in photoluminescence 有权

    公开(公告)号：US11072820B2

    公开(公告)日：2021-07-27

    申请号：US16159488

    申请日：2018-10-12

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Eli A. Hefner ,  Dianna Maar

    IPC: C12Q1/6844 ,  C12Q1/6816 ,  G01N21/64

    Abstract: Methods of, and compositions for, analysis using digital amplification assays with unconventional/inverse changes in photoluminescence to indicate the presence of one or more targets. In an exemplary method, isolated volumes may be formed, only a subset of which contain a target. Each volume may include a probe having a label, a sink having a quencher and configured to hybridize with the probe to quench the label, and a separator configured to hybridize with the probe and/or the sink to block hybridization of the sink with the probe. An amplification reaction may be performed in the volumes to generate an amplicon corresponding to the target. The separator may hybridize with the amplicon, and may be extended or degraded by the amplification reaction. Photoluminescence of the label may be detected from the volumes, and the photoluminescence of target-positive volumes may be less than that of target-negative volumes.

    Amplification reporter with base-pairing oligomers
    20.
    发明授权
    Amplification reporter with base-pairing oligomers 有权

    公开(公告)号：US10604789B2

    公开(公告)日：2020-03-31

    申请号：US15411482

    申请日：2017-01-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

    IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

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