公开(公告)号：US08779387B2

公开(公告)日：2014-07-15

申请号：US13033299

申请日：2011-02-23

Applicant: Clare E. Rogers ,  Jack T. Ball ,  Collin A. Rich

Inventor： Clare E. Rogers ,  Jack T. Ball ,  Collin A. Rich

IPC: G01J1/58 ,  G01N21/64

CPC classification number: G01N15/1429 ,  G01N15/1459

Abstract: A method for detecting fluorochromes in a flow cytometer, including: receiving a sample including particles each tagged with at least one of a first fluorochrome and a second fluorochrome, in which the first and second fluorochromes having distinct spillover coefficients; detecting the particles, including detecting the first and second fluorochromes with a first detector and a second detector; forming a data set for detected particles based on the detection of the first and second fluorochromes; characterizing a detected spillover coefficient for each detected fluorochrome from the data set; and sorting the detected particles into predicted fluorochrome populations based on the detected spillover coefficients. A system for detecting fluorochromes in a flow cytometer, including a flow cell, a fixed gain detection system, and a processor that generates a detected spillover coefficient for each detected particle and sorts the detected particle into predicted fluorochrome populations based on the detected spillover coefficient.

Abstract translation: 一种用于在流式细胞仪中检测荧光染料的方法，包括：接收包含每个标记有第一荧光染料和第二荧光染料中的至少一种的颗粒的样品，其中第一和第二荧光染料具有不同的溢出系数; 检测颗粒，包括用第一检测器和第二检测器检测第一和第二荧光染料; 基于第一和第二荧光色素的检测形成检测到的颗粒的数据集; 表征来自数据集的每个检测到的荧光染料的检测到的溢出系数; 并且基于检测到的溢出系数将检测到的颗粒分选成预测的荧光染色体群体。 一种用于在流式细胞仪中检测荧光染料的系统，包括流动池，固定增益检测系统和处理器，其产生每个检测到的颗粒的检测到的溢出系数，并且基于检测到的溢出系数将检测到的颗粒分解成预测的荧光染色体群。

公开(公告)号：US08658453B2

公开(公告)日：2014-02-25

申请号：US11612659

申请日：2006-12-19

Applicant: David F. Lemmerhirt ,  Collin A. Rich

Inventor： David F. Lemmerhirt ,  Collin A. Rich

IPC: H01L21/00

CPC classification number: B06B1/0292

Abstract: The first integrated circuit/transducer device 36 of the handheld probe includes CMOS circuits 110 and cMUT elements 112. The cMUT elements 112 function to generate an ultrasonic beam, detect an ultrasonic echo, and output electrical signals, while the CMOS circuits 110 function to perform analog or digital operations on the electrical signals generated through operation of the cMUT elements 112. The manufacturing method for the first integrated circuit/transducer device 36 of the preferred embodiment includes the steps of depositing the lower electrode S102; depositing a sacrificial layer S104; depositing a dielectric layer S106; removing the sacrificial layer S108, followed by the steps of depositing the upper electrode S110 and depositing a protective layer on the upper electrode S112.

Abstract translation: 手持式探头的第一集成电路/传感器装置36包括CMOS电路110和cMUT元件112.CMUT元件112用于产生超声波束，检测超声回波并输出电信号，同时CMOS电路110用于执行 对通过cMUT元件112的操作产生的电信号进行模拟或数字操作。优选实施例的第一集成电路/换能器装置36的制造方法包括以下步骤：沉积下电极S102; 沉积牺牲层S104; 沉积介电层S106; 去除牺牲层S108，随后沉积上电极S110并在上电极S112上沉积保护层的步骤。

公开(公告)号：US08432541B2

公开(公告)日：2013-04-30

申请号：US12939836

申请日：2010-11-04

Applicant: Collin A. Rich

Inventor： Collin A. Rich

IPC: G01N1/10

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G02B27/141 ,  G02B27/145

Abstract: The optical system of the preferred embodiments includes a first light source that creates a first beam of a first wavelength, a first collimating element that collimates the first beam, a second light source 102 that creates a second beam of a second wavelength, a second collimating element that collimates the second beam, a beam combining element that combines the collimated beams, and a focusing element that focuses the combined collimated beam to a single point.

Abstract translation: 优选实施例的光学系统包括产生第一波长的第一光束的第一光源，准直第一光束的第一准直元件，产生第二波长的第二光束的第二光源102，第二准直 对第二光束进行准直的元件，组合准直光束的光束组合元件以及将组合的准直光束聚焦到单个点的聚焦元件。

公开(公告)号：US08309428B2

公开(公告)日：2012-11-13

申请号：US11612656

申请日：2006-12-19

Applicant: David F. Lemmerhirt ,  Collin A. Rich

Inventor： David F. Lemmerhirt ,  Collin A. Rich

IPC: H01L21/30

CPC classification number: B06B1/0292 ,  A61B8/00 ,  A61B8/4472

Abstract: The first integrated circuit/transducer device 36 of the handheld probe includes CMOS circuits 110 and cMUT elements 112. The cMUT elements 112 function to generate an ultrasonic beam, detect an ultrasonic echo, and output electrical signals, while the CMOS circuits 110 function to perform analog or digital operations on the electrical signals generated through operation of the cMUT elements 112. The manufacturing method for the first integrated circuit/transducer device 36 of the preferred embodiment includes the steps of depositing the lower electrode S102; depositing a sacrificial layer S104; depositing a dielectric layer S106; depositing the upper electrode S108; depositing a protective layer on the upper electrode S110; and removing the sacrificial layer S112. In the preferred embodiment, the manufacturing method also includes the step of depositing a sealant layer to seal a cavity between the lower electrode and the upper electrode S114.

Abstract translation: 手持式探头的第一集成电路/传感器装置36包括CMOS电路110和cMUT元件112.CMUT元件112用于产生超声波束，检测超声回波并输出电信号，同时CMOS电路110用于执行 对通过cMUT元件112的操作产生的电信号进行模拟或数字操作。优选实施例的第一集成电路/换能器装置36的制造方法包括以下步骤：沉积下电极S102; 沉积牺牲层S104; 沉积介电层S106; 沉积上电极S108; 在上电极S110上沉积保护层; 并去除牺牲层S112。 在优选实施例中，制造方法还包括沉积密封剂层以密封下电极和上电极S114之间的腔的步骤。

公开(公告)号：US08303894B2

公开(公告)日：2012-11-06

申请号：US12857290

申请日：2010-08-16

Applicant: Collin A. Rich

Inventor： Collin A. Rich

IPC: G01N21/00 ,  G01N1/00 ,  C12M1/36 ,  C12Q3/00 ,  B08B7/00 ,  G01N35/08

CPC classification number: G01N1/14 ,  G01N15/06 ,  G01N15/1404 ,  G01N2015/1413 ,  G05D7/0682 ,  Y10T137/0402 ,  Y10T137/85978 ,  Y10T137/85986 ,  Y10T436/115831 ,  Y10T436/117497

Abstract: The fluidic system including a sheath pump that pumps sheath fluid from a sheath container into an interrogation zone, a waste pump that pumps waste fluid from the interrogation zone to a waste container, in which the flow rate of the sheath fluid is different from the flow rate of the waste fluid thereby drawing a sample fluid from a sample container into the interrogation zone, a detection system that provides a data set of input signals from the sample fluid, an analysis engine that recognizes aggregate particle events in the data set, and a controller that automatically adjusts the flow rate of the sample fluid into the interrogation zone based on the recognition of aggregate particle events, by controlling at least one of the flow rates of the sheath fluid and the waste fluid.

Abstract translation: 流体系统包括将皮套流体从护套容器泵送到询问区域的护套泵，将废液从询问区泵送到废物容器的废水泵，其中护套流体的流速不同于流动 从而将样品流体从样品容器抽入询问区，提供来自样品流体的输入信号的数据集的检测系统，识别数据集中的聚集粒子事件的分析引擎和 控制器，通过控制鞘液和废液的流速中的至少一个，基于聚集颗粒事件的识别，自动调节进入询问区的样品流体的流量。

公开(公告)号：US08283177B2

公开(公告)日：2012-10-09

申请号：US12476860

申请日：2009-06-02

Applicant: Jack T. Ball ,  Aaron Kehrer ,  Nathaniel C. Bair ,  Collin A. Rich

Inventor： Jack T. Ball ,  Aaron Kehrer ,  Nathaniel C. Bair ,  Collin A. Rich

IPC: G01N21/15 ,  G01N21/09 ,  G01N21/05 ,  G01N35/08

CPC classification number: G01N15/1404 ,  B01L3/02 ,  B01L3/5027 ,  B01L99/00 ,  G01N2015/1413 ,  G05D7/0682 ,  Y10T436/25375

Abstract: A method for cleaning a fluidic system of a flow cytometer having a sheath pump to pump sheath fluid towards an interrogation zone and a waste pump to pump the sheath fluid and a sample fluid as waste fluid from the interrogation zone, wherein the sheath pump and/or the waste pump draw sample fluid into the flow cytometer through a drawtube towards the interrogation zone. The method includes controlling the sheath pump and the waste pump to cooperatively flush a fluid out through the drawtube, thereby cleaning the fluidic system of the flow cytometer.

Abstract translation: 一种用于清洗流式细胞仪的流体系统的方法，所述流式细胞仪具有护套泵以将鞘液朝向询问区泵送，而废泵泵送鞘液和作为来自询问区的废液的样品流体，其中护套泵和/ 或废泵将样品流体通过拉管朝向询问区抽入流式细胞仪。 该方法包括控制护套泵和废水泵通过抽吸管共同冲洗流体，从而清洗流式细胞仪的流体系统。

公开(公告)号：US08229684B2

公开(公告)日：2012-07-24

申请号：US12770341

申请日：2010-04-29

Applicant: Clement James Goebel ,  Collin A. Rich

Inventor： Clement James Goebel ,  Collin A. Rich

IPC: G06F17/40 ,  G06F17/00

CPC classification number: G01N15/1459 ,  G01N2015/0092 ,  G01N2015/1477

Abstract: The detection system of the first preferred embodiment includes a detector, having a wide dynamic range, that receives photonic inputs from an interrogation zone and produces an analog signal; and an analog-to-digital converter (ADC), having a high bit resolution, that is coupled to the detector and converts an analog signal to a digital signal. The digital signal includes an initial data set of the full dynamic range of the input signals from the flow cytometer sample. The method of extracting and analyzing data from a flow cytometer system of the first preferred embodiment preferably includes the steps of: collecting a full dynamic range of input signals from a flow cytometer sample; recognizing and annotating aggregate particle events; and storing an initial data set and an annotated data set of the full dynamic range of the input signals from the flow cytometer sample.

Abstract translation: 第一优选实施例的检测系统包括具有宽动态范围的检测器，其接收来自询问区的光子输入并产生模拟信号; 以及具有高比特分辨率的模数转换器（ADC），其耦合到检测器并将模拟信号转换为数字信号。 数字信号包括来自流式细胞仪样本的输入信号的完整动态范围的初始数据集。 从第一优选实施例的流式细胞仪系统提取和分析数据的方法优选地包括以下步骤：从流式细胞仪样品收集输入信号的完整动态范围; 识别和注释聚集粒子事件; 并存储来自流式细胞仪样本的输入信号的完整动态范围的初始数据集和注释数据集。

公开(公告)号：US08004674B2

公开(公告)日：2011-08-23

申请号：US12476877

申请日：2009-06-02

Applicant: Jack T. Ball ,  Collin A. Rich ,  Clare E. Rogers

Inventor： Jack T. Ball ,  Collin A. Rich ,  Clare E. Rogers

IPC: G01J3/30

CPC classification number: G01J3/4406

Abstract: A system for a flow cytometer that collects data for a sample prepared with a plurality of fluorochromes that includes a fixed gain detection system that collects data for a plurality of fluorescence channels, fluorochrome compensation factors for a plurality of fluorochromes types, and a computer system that has an interface that gathers fluorochrome information of the sample and an analysis program that compensates for spectral spillover in the collected data. The fixed gain detection system preferably has a wide dynamic range. A fluorochrome compensation factor preferably remains constant for a fixed gain detection system. The analysis program preferably uses the fluorochrome compensation factors to compensate for spectral spillover.

Abstract translation: 一种用于流式细胞计数器的系统，其收集由多个荧光染料制备的样品的数据，所述荧光染料包括收集多个荧光通道的数据的固定增益检测系统，用于多个荧光染料类型的荧光染料补偿因子，以及计算机系统， 具有收集样品的荧光染料信息的界面和补偿收集的数据中的光谱外溢的分析程序。 固定增益检测系统优选具有宽的动态范围。 对于固定增益检测系统，荧光染料补偿因子优选保持恒定。 分析程序优选使用荧光染料补偿因子来补偿光谱溢出。

公开(公告)号：US20110061471A1

公开(公告)日：2011-03-17

申请号：US12942846

申请日：2010-11-09

Applicant: Collin A. Rich ,  Jack T. Ball ,  Grant C. Howes

Inventor： Collin A. Rich ,  Jack T. Ball ,  Grant C. Howes

IPC: G01N1/20

CPC classification number: G01N15/14

Abstract: A system and method for a flow cytometer system including a sheath pump that pumps sheath fluid from a sheath container into an interrogation zone, a waste pump that pumps waste fluid from the interrogation zone into a waste container, wherein the sheath pump and waste pump cooperatively and simultaneously draw sample fluid from a sample container into the interrogation zone, a controller that adjusts the flow rate of the sample fluid from the sample container into the interrogation zone, and a sensor system that coordinates with the controller to measure the amount of sample fluid in the sample container when the controller substantially pauses the sample fluid flow from the sample container into the interrogation zone. The system may further include a processor that monitors a measured volume of sample fluid introduced into the flow cytometer and an expected sample volume.

Abstract translation: 一种用于流式细胞仪系统的系统和方法，包括将鞘液从皮肤容器泵送到询问区的鞘泵，将废液从询问区泵送到废液容器中的废泵，其中鞘泵和废泵协同 同时将样品流体从样品容器中抽出到询问区中，控制器调节样品流体从样品容器进入询问区的流量，以及与控制器协调测量样品流量的传感器系统 在样品容器中，当控制器基本上暂停样品流体从样品容器进入询问区时。 该系统还可以包括处理器，其监测引入流式细胞仪中的样品流体的测量体积和预期的样品体积。

公开(公告)号：US07857005B2

公开(公告)日：2010-12-28

申请号：US12425830

申请日：2009-04-17

Applicant: Collin A. Rich ,  Nathaniel C. Bair

Inventor： Collin A. Rich ,  Nathaniel C. Bair

IPC: F16L55/04

CPC classification number: F16L55/04 ,  F04B43/12 ,  F15B1/021 ,  F15B1/10 ,  F15B21/008 ,  F15B2201/205 ,  F15B2201/215 ,  F15B2201/3151 ,  G01N15/1404 ,  G01N15/1459 ,  Y10T137/3118

Abstract: A pulsation attenuator for a fluidic system with a fluidic pump. The pulsation attenuator includes a fluidic channel, a first fluidic device adapted to attenuate pulsations, and a second fluidic device adapted to attenuate pulsations. Preferably, the first fluidic device includes a first fluidic resistor and a first fluidic capacitor, and the second fluidic device includes a second fluidic resistor and a second fluidic capacitor. Preferably, the first fluidic resistor and second fluidic resistor are resistive channels. Preferably, the first fluidic capacitor and second fluidic capacitor include a membrane that expands and accumulates fluid and then contracts and reintroduces the accumulated fluid into the fluidic channel.

Abstract translation: 一种具有流体泵的流体系统的脉动衰减器。 脉动衰减器包括流体通道，适于衰减脉动的第一流体装置，以及适于衰减脉动的第二流体装置。 优选地，第一流体装置包括第一流体电阻器和第一流体电容器，并且第二流体装置包括第二流体电阻器和第二流体电容器。 优选地，第一流体电阻器和第二流体电阻器是电阻通道。 优选地，第一流体电容器和第二流体电容器包括膨胀和积聚流体然后收缩并将积聚的流体再引入流体通道的膜。