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公开(公告)号：US20170283865A1
公开(公告)日：2017-10-05
申请号：US15249684
申请日：2016-08-29
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew A. Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
CPC classification number: C12Q1/6858 , A01G24/00 , C12N5/00 , C12N15/1082 , C12N15/66 , C12N15/70 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B40/08
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
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公开(公告)号：US20160130595A1
公开(公告)日：2016-05-12
申请号：US14938643
申请日：2015-11-11
Applicant: Dow AgroSciences LLC
Inventor： Sandeep Kumar , Toby Cicak , Heather Leigh Robinson , Dayakar Reddy Pareddy , Wei Chen
IPC: C12N15/82
CPC classification number: C12N15/8216
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Arabidopsis thaliana Ubiquitin-10 gene promoter or Cassava Vein Mosaic Virus promoter, and the full-length nucleotide sequence elements from a Cassava Vein Mosaic Virus promoter. Some embodiments relate to a synthetic CsVMV bi-directional promoter that functions in plants to promote transcription of two operably linked nucleotide sequences.
Abstract translation: 本公开涉及用于促进植物或植物细胞中核苷酸序列的转录的组合物和方法，其使用来自拟南芥泛素-10基因启动子或木薯浆静脉花叶病毒启动子的最小核心启动子元件，以及全长核苷酸序列元件 从木薯静脉花叶病毒启动子。 一些实施方案涉及在植物中起作用以促进两个可操作连接的核苷酸序列的转录的合成的CsVMV双向启动子。
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公开(公告)号：US20140298547A1
公开(公告)日：2014-10-02
申请号：US14105223
申请日：2013-12-13
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
IPC: C12Q1/68
CPC classification number: C12Q1/6858 , A01G24/00 , C12N5/00 , C12N15/1082 , C12N15/66 , C12N15/70 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B40/08
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。
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公开(公告)号：US10273494B2
公开(公告)日：2019-04-30
申请号：US15484581
申请日：2017-04-11
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Sandeep Kumar , Wei Chen
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
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公开(公告)号：US09994919B2
公开(公告)日：2018-06-12
申请号：US14403648
申请日：2013-05-29
Applicant: DOW AGROSCIENCES LLC , Wei Chen , Nathan Van Opdorp , Steve Plehn , Nadia Chaidir
Inventor： Wei Chen , Nathan Van Opdorp , Steve Plehn , Peter Friedemann , Nadia Chaidir , Siva Prasad Kumpatla
IPC: C12Q1/6895 , C12Q1/68
CPC classification number: C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158
Abstract: The disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more floury2 (fl2) mutations. In embodiments, the disclosure concerns a gene specific PCR-based molecular (KASPar®) assay for identifying the floury2 trait in plants. In certain embodiments, compositions and methods are disclosed for determining the zygosity of corn plants with respect to the fl2 allele. In particular embodiments, the assay may be used for fl2 germplasm identification, accelerating introgression and molecular breeding programs in corn and other plants.
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Patent Agency Ranking
公开(公告)号：US20170137834A1
公开(公告)日：2017-05-18
申请号：US15331126
申请日：2016-10-21
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Sandeep Kumar , Navin Elango , Jeffrey Beringer , Shavell Gorman , Andrew F. Worden , Sara Bennett , Daren Hemingway , Wei Chen , Huixia Wu , Ning Zhou , Michelle Sprint Smith
IPC: C12N15/82 , C07K14/325
CPC classification number: C12N15/8227 , C12N9/14 , C12N15/8225 , C12N15/8286 , C12Y303/01001 , Y02A40/162
Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays GRMZM2G015295 gene regulatory elements.
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公开(公告)号：US10968455B2
公开(公告)日：2021-04-06
申请号：US16271986
申请日：2019-02-11
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Sandeep Kumar , Wei Chen
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
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公开(公告)号：US20190161761A1
公开(公告)日：2019-05-30
申请号：US16271986
申请日：2019-02-11
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Sandeep Kumar , Wei Chen
IPC: C12N15/82
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
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公开(公告)号：US20170334956A1
公开(公告)日：2017-11-23
申请号：US15484581
申请日：2017-04-11
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Sandeep Kumar , Wei Chen
IPC: C07K14/415 , C12N15/82 , C12N15/00
CPC classification number: C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8227 , C12N15/823
Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
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公开(公告)号：US09493844B2
公开(公告)日：2016-11-15
申请号：US14105223
申请日：2013-12-13
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
IPC: C12Q1/68
CPC classification number: C12Q1/6858 , A01G24/00 , C12N5/00 , C12N15/1082 , C12N15/66 , C12N15/70 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B40/08
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。
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