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公开(公告)号:US20090286245A1
公开(公告)日:2009-11-19
申请号:US12414191
申请日:2009-03-30
申请人: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
发明人: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6874 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101 , C12Q2565/1015 , C12Q2527/137 , C12Q2521/101
摘要: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
摘要翻译: 用于核苷酸测序的组合物,试剂盒,方法和系统,其包括产生聚合酶反应,其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶,包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。
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公开(公告)号:US20110183320A1
公开(公告)日:2011-07-28
申请号:US12945767
申请日:2010-11-12
申请人: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
发明人: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译: 提供了方法,组合物和系统用于表征修饰的核酸。 在某些优选实施方案中,提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基,酶修饰的碱基,脱碱基位点,非天然碱基,二级结构和与模板核酸结合的试剂。
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公开(公告)号:US09175338B2
公开(公告)日:2015-11-03
申请号:US12945767
申请日:2010-11-12
申请人: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
发明人: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译: 提供了方法,组合物和系统用于表征修饰的核酸。 在某些优选实施方案中,提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基,酶修饰的碱基,脱碱基位点,非天然碱基,二级结构和与模板核酸结合的试剂。
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公开(公告)号:US20100221716A1
公开(公告)日:2010-09-02
申请号:US12635618
申请日:2009-12-10
申请人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes
发明人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译: 提供了方法,组合物和系统用于表征修饰的核酸。 在某些优选实施方案中,提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基,酶修饰的碱基,脱碱基位点,非天然碱基,二级结构和与模板核酸结合的试剂。
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公开(公告)号:US09175341B2
公开(公告)日:2015-11-03
申请号:US12635618
申请日:2009-12-10
申请人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
发明人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
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公开(公告)号:US20130029853A1
公开(公告)日:2013-01-31
申请号:US13633673
申请日:2012-10-02
申请人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
发明人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译: 提供了方法,组合物和系统用于表征修饰的核酸。 在某些优选实施方案中,提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基,酶修饰的碱基,脱碱基位点,非天然碱基,二级结构和与模板核酸结合的试剂。
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公开(公告)号:US08133672B2
公开(公告)日:2012-03-13
申请号:US12414191
申请日:2009-03-30
申请人: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
发明人: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
CPC分类号: C12Q1/6869 , C12Q1/6874 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101 , C12Q2565/1015 , C12Q2527/137 , C12Q2521/101
摘要: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
摘要翻译: 用于核苷酸测序的组合物,试剂盒,方法和系统,其包括产生聚合酶反应,其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶,包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。
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公开(公告)号:US20090208957A1
公开(公告)日:2009-08-20
申请号:US12315626
申请日:2008-12-03
申请人: Jonas Korlach , Daniel Roitman , John Eid , Geoff Otto , Paul Hardenbol , Benjamin Flusberg
发明人: Jonas Korlach , Daniel Roitman , John Eid , Geoff Otto , Paul Hardenbol , Benjamin Flusberg
CPC分类号: G01N33/54373 , C12Q1/6874 , G01N33/542 , G01N33/54313 , G01N33/573 , G01N33/58 , G01N33/585 , C12Q2565/607 , C12Q2565/531 , C12Q2563/143 , C12Q2563/137
摘要: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.
摘要翻译: 描述了增强荧光标记策略的系统和方法以及使用非荧光和/或非光学标记策略的系统和方法,例如与使用ZMW的单分子测序一样。
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公开(公告)号:US09063156B2
公开(公告)日:2015-06-23
申请号:US12814075
申请日:2010-06-11
申请人: Jonas Korlach , Stephen Turner , Benjamin Flusberg , Mark Chaisson , Eric Schadt , Jeffrey Wegener
发明人: Jonas Korlach , Stephen Turner , Benjamin Flusberg , Mark Chaisson , Eric Schadt , Jeffrey Wegener
IPC分类号: C12Q1/68 , G01N33/53 , G01N33/68 , G01N33/542 , G01N33/543
CPC分类号: G01N33/68 , C12Q1/6825 , C12Q1/6827 , C12Q1/6869 , G01N33/542 , G01N33/54306 , G01N33/54373 , G01N33/582 , G01N33/6875 , G01N2333/705 , G01N2458/10 , C12Q2563/107 , C12Q2522/101 , C12Q2561/113 , C12Q2537/164 , C12Q2523/125
摘要: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions, and for determining various characteristics of the different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to stimulate, enhance, or inhibit such reactions.
摘要翻译: 本发明一般涉及用于进行各种不同生物反应的单分子实时分析以及用于确定不同生物反应的各种特征的组合物,方法和系统。 分析这些反应的能力提供研究这些反应的机会,并且潜在地鉴定影响这些反应的因素和/或方法,例如刺激,增强或抑制这些反应。
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公开(公告)号:US20100323912A1
公开(公告)日:2010-12-23
申请号:US12814075
申请日:2010-06-11
申请人: Jonas Korlach , Stephen Turner , Benjamin Flusberg , Mark Chaisson , Eric Schadt , Jeffrey Wegener
发明人: Jonas Korlach , Stephen Turner , Benjamin Flusberg , Mark Chaisson , Eric Schadt , Jeffrey Wegener
IPC分类号: C40B30/04 , C12Q1/68 , G01N33/566 , C40B50/14
CPC分类号: G01N33/68 , C12Q1/6825 , C12Q1/6827 , C12Q1/6869 , G01N33/542 , G01N33/54306 , G01N33/54373 , G01N33/582 , G01N33/6875 , G01N2333/705 , G01N2458/10 , C12Q2563/107 , C12Q2522/101 , C12Q2561/113 , C12Q2537/164 , C12Q2523/125
摘要: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions, and for determining various characteristics of the different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to stimulate, enhance, or inhibit such reactions.
摘要翻译: 本发明一般涉及用于进行各种不同生物反应的单分子实时分析以及用于确定不同生物反应的各种特征的组合物,方法和系统。 分析这些反应的能力提供研究这些反应的机会,并且潜在地鉴定影响这些反应的因素和/或方法,例如刺激,增强或抑制这些反应。
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