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公开(公告)号:US09175338B2
公开(公告)日:2015-11-03
申请号:US12945767
申请日:2010-11-12
申请人: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
发明人: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译: 提供了方法,组合物和系统用于表征修饰的核酸。 在某些优选实施方案中,提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基,酶修饰的碱基,脱碱基位点,非天然碱基,二级结构和与模板核酸结合的试剂。
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公开(公告)号:US09175341B2
公开(公告)日:2015-11-03
申请号:US12635618
申请日:2009-12-10
申请人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
发明人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
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公开(公告)号:US20130029853A1
公开(公告)日:2013-01-31
申请号:US13633673
申请日:2012-10-02
申请人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
发明人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译: 提供了方法,组合物和系统用于表征修饰的核酸。 在某些优选实施方案中,提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基,酶修饰的碱基,脱碱基位点,非天然碱基,二级结构和与模板核酸结合的试剂。
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公开(公告)号:US20110183320A1
公开(公告)日:2011-07-28
申请号:US12945767
申请日:2010-11-12
申请人: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
发明人: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译: 提供了方法,组合物和系统用于表征修饰的核酸。 在某些优选实施方案中,提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基,酶修饰的碱基,脱碱基位点,非天然碱基,二级结构和与模板核酸结合的试剂。
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公开(公告)号:US20100221716A1
公开(公告)日:2010-09-02
申请号:US12635618
申请日:2009-12-10
申请人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes
发明人: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译: 提供了方法,组合物和系统用于表征修饰的核酸。 在某些优选实施方案中,提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基,酶修饰的碱基,脱碱基位点,非天然碱基,二级结构和与模板核酸结合的试剂。
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公开(公告)号:US08986928B2
公开(公告)日:2015-03-24
申请号:US12757789
申请日:2010-04-09
申请人: Stephen Turner , Benjamin Flusberg , Mathieu Foquet , Hans Callebaut , Robert Sebra , Bidhan Chaudhuri , Jon Sorenson , Keith Bjornson , Adrian Fehr , Jonas Korlach , Robin Emig
发明人: Stephen Turner , Benjamin Flusberg , Mathieu Foquet , Hans Callebaut , Robert Sebra , Bidhan Chaudhuri , Jon Sorenson , Keith Bjornson , Adrian Fehr , Jonas Korlach , Robin Emig
IPC分类号: C12Q1/68 , G01N27/447 , G01N33/487 , G01N33/50
CPC分类号: G01N33/48721 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , C12Q1/6874 , C12Q2565/631 , G01N27/447 , G01N27/44791 , C12Q2563/116 , C12Q2527/113
摘要: The invention relates to devices and methods for nanopore sequencing. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.
摘要翻译: 本发明涉及纳米孔测序的装置和方法。 本发明包括纳入电子电路的纳米孔阵列,例如在CMOS中。 在一些情况下,纳米孔阵列包括用于隔离电子信号的电阻开口以改善测序。 公开了通过纳米孔控制易位的方法。
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公开(公告)号:US20100331194A1
公开(公告)日:2010-12-30
申请号:US12757789
申请日:2010-04-09
申请人: Stephen Turner , Benjamin Flusberg , Mathieu Foquet , Hans Callebaut , Robert Sebra , Bidhan Chaudhuri , Jon Sorenson , Keith Bjornson , Adrian Fehr , Jonas Korlach , Robin Emig
发明人: Stephen Turner , Benjamin Flusberg , Mathieu Foquet , Hans Callebaut , Robert Sebra , Bidhan Chaudhuri , Jon Sorenson , Keith Bjornson , Adrian Fehr , Jonas Korlach , Robin Emig
CPC分类号: G01N33/48721 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , C12Q1/6874 , C12Q2565/631 , G01N27/447 , G01N27/44791 , C12Q2563/116 , C12Q2527/113
摘要: The invention relates to devices and methods for nanopore sequencing. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.
摘要翻译: 本发明涉及纳米孔测序的装置和方法。 本发明包括纳入电子电路的纳米孔阵列,例如在CMOS中。 在一些情况下,纳米孔阵列包括用于隔离电子信号的电阻开口以改善测序。 公开了通过纳米孔控制易位的方法。
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公开(公告)号:US20090286245A1
公开(公告)日:2009-11-19
申请号:US12414191
申请日:2009-03-30
申请人: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
发明人: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , C12Q1/6874 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101 , C12Q2565/1015 , C12Q2527/137 , C12Q2521/101
摘要: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
摘要翻译: 用于核苷酸测序的组合物,试剂盒,方法和系统,其包括产生聚合酶反应,其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶,包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。
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公开(公告)号:US08133672B2
公开(公告)日:2012-03-13
申请号:US12414191
申请日:2009-03-30
申请人: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
发明人: Keith Bjornson , Harold Lee , Jonas Korlach , Stephen Turner , Arek Bibillo , Fred Christians , Robin Emig , Molly He , Insil Park , Lei Jia , Andrei Fedorov , John Lyle
CPC分类号: C12Q1/6869 , C12Q1/6874 , C12Q2527/125 , C12Q2527/119 , C12Q2527/101 , C12Q2565/1015 , C12Q2527/137 , C12Q2521/101
摘要: Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
摘要翻译: 用于核苷酸测序的组合物,试剂盒,方法和系统,其包括产生聚合酶反应,其在聚合酶反应的可观察期内显示两个动力学可观察步骤。 可以通过例如选择合适的聚合酶,包括辅因子的聚合酶反应条件和包括引发的模板和核苷酸的聚合酶反应底物来产生两个缓慢的步骤系统。
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公开(公告)号:US09063156B2
公开(公告)日:2015-06-23
申请号:US12814075
申请日:2010-06-11
申请人: Jonas Korlach , Stephen Turner , Benjamin Flusberg , Mark Chaisson , Eric Schadt , Jeffrey Wegener
发明人: Jonas Korlach , Stephen Turner , Benjamin Flusberg , Mark Chaisson , Eric Schadt , Jeffrey Wegener
IPC分类号: C12Q1/68 , G01N33/53 , G01N33/68 , G01N33/542 , G01N33/543
CPC分类号: G01N33/68 , C12Q1/6825 , C12Q1/6827 , C12Q1/6869 , G01N33/542 , G01N33/54306 , G01N33/54373 , G01N33/582 , G01N33/6875 , G01N2333/705 , G01N2458/10 , C12Q2563/107 , C12Q2522/101 , C12Q2561/113 , C12Q2537/164 , C12Q2523/125
摘要: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions, and for determining various characteristics of the different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to stimulate, enhance, or inhibit such reactions.
摘要翻译: 本发明一般涉及用于进行各种不同生物反应的单分子实时分析以及用于确定不同生物反应的各种特征的组合物,方法和系统。 分析这些反应的能力提供研究这些反应的机会,并且潜在地鉴定影响这些反应的因素和/或方法,例如刺激,增强或抑制这些反应。
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