公开(公告)号：US4933275A

公开(公告)日：1990-06-12

申请号：US791114

申请日：1985-10-24

申请人： Jack R. Wands ,  Mehmet Ozturk ,  Dominique Bellet

发明人： Jack R. Wands ,  Mehmet Ozturk ,  Dominique Bellet

IPC分类号： C07K14/195 ,  C07K14/005 ,  C07K14/575 ,  C07K16/00 ,  C07K16/26 ,  C07K19/00 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/53 ,  G01N33/577

CPC分类号： C07K16/26 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/814 ,  Y10S436/817 ,  Y10S436/818

摘要： A method for the determination of a free protein subunit of a quaternary protein in a sample, which comprises:(a) contacting a sample with a first immunological binding partner which is or will be bound to a carrier, wherein the first immunological binding partner binds epitopic determinants bindable only on the free protein subunit;(b) incubating the components of step (a) for a period of time and under conditions sufficient to form an immune complex between the free protein subunit, the first immunological binding partner, and the carrier;(c) separating the carrier of step (b) from the sample;(d) adding to the carrier of step (c), a detectably-labeled second immunological binding partner, wherein the second immunological binding partner binds epitopic determinants bindable on both the free protein subunit and the quaternary protein; and(e) determining the detectably-labeled second immunological binding partner in the carrier or in liquid phase.

摘要翻译： 一种用于测定样品中季蛋白质的游离蛋白质亚基的方法，其包括：（a）使样品与第一免疫结合配偶体接触，所述第一免疫结合配偶体与载体结合或将结合到载体上，其中所述第一免疫结合配偶体结合 仅在游离蛋白亚基上结合的表位决定簇; （b）将步骤（a）的组分在足以在游离蛋白质亚基，第一免疫结合配偶体和载体之间形成免疫复合物的条件下孵育一段时间; （c）将步骤（b）的载体与样品分离; （d）向步骤（c）的载体添加可检测标记的第二免疫结合配偶体，其中所述第二免疫结合配偶体结合可结合游离蛋白质亚基和季蛋白的表位决定簇; 和（e）确定载体中或液相中的可检测标记的第二免疫结合配偶体。