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公开(公告)号：US11033839B2
公开(公告)日：2021-06-15
申请号：US16208018
申请日：2018-12-03
Applicant: EMD Millipore Corporation
Inventor： Jonathan Steen , Mikhail Kozlov , Herbert Lutz , Joseph Parrella , Elizabeth M. Goodrich , Bala Raghunath
Abstract: A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.
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公开(公告)号：US20210170336A1
公开(公告)日：2021-06-10
申请号：US16762688
申请日：2018-11-01
Applicant: EMD Millipore Corporation
Inventor： Elizabeth M. Goodrich , Akshat Gupta , Herbert Lutz
Abstract: Processes and systems for filtering a liquid sample are provided. Batches of a liquid sample can be routed to two or more cycling tanks (e.g., first and second cycling tanks). Upon filling a first cycling tank, a first batch of the liquid sample can be routed to a filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the first cycling tank or to a collection vessel. Upon filling a second cycling tank, a second batch of the liquid sample is routed to the filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the second cycling tank or to the collection vessel. The filling and continuous diafiltration of batches of the liquid sample continues to alternate between the two or more cycling tanks until a total product volume is processed.
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公开(公告)号：US20190099717A1
公开(公告)日：2019-04-04
申请号：US16207895
申请日：2018-12-03
Applicant: EMD Millipore Corporation
Inventor： Herbert Lutz , Joseph Parrella , Bala Raghunath
IPC: B01D61/14
Abstract: The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.
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公开(公告)号：US12297229B2
公开(公告)日：2025-05-13
申请号：US16259489
申请日：2019-01-28
Applicant: EMD Millipore Corporation
Inventor： Venkatesh Natarajan , Herbert Lutz , Bala Raghunath
IPC: C07K1/34 , B01D15/08 , B01D15/12 , B01D61/14 , C07K1/18 , C07K1/22 , C07K1/36 , C07K16/00 , C07K16/06 , A61L2/00
Abstract: In various embodiments, the present invention provides a process for separating target proteins from non-target proteins in a sample comprising increasing the concentration of the target proteins and non-target proteins in the sample and subsequently delivering the concentrated sample to a chromatography device. In other embodiments, the invention relates to a process for increasing the capacity of a chromatography device for a target protein by delivering a concentrated sample comprising the target protein to a chromatography device.
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公开(公告)号：US20230211291A1
公开(公告)日：2023-07-06
申请号：US18183609
申请日：2023-03-14
Applicant: EMD Millipore Corporation
Inventor： Elizabeth M. Goodrich , Akshat Gupta , Herbert Lutz
CPC classification number: B01D61/146 , B01D61/18 , B01D61/22 , B01D65/02 , B01D2311/04 , B01D2311/08 , B01D2311/25 , B01D2313/18 , B01D2313/243 , B01D2313/50 , B01D2315/10 , B01D2315/14 , B01D2315/16 , B01D2317/022 , B01D2317/04 , B01D2321/16
Abstract: Processes and systems for filtering a liquid sample are provided. Batches of a liquid sample can be routed to two or more cycling tanks (e.g., first and second cycling tanks). Upon filling a first cycling tank, a first batch of the liquid sample can be routed to a filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the first cycling tank or to a collection vessel. Upon filling a second cycling tank, a second batch of the liquid sample is routed to the filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the second cycling tank or to the collection vessel. The filling and continuous diafiltration of batches of the liquid sample continues to alternate between the two or more cycling tanks until a total product volume is processed.
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Patent Agency Ranking
公开(公告)号：US20190153027A1
公开(公告)日：2019-05-23
申请号：US16259489
申请日：2019-01-28
Applicant: EMD Millipore Corporation
Inventor： Venkatesh Natarajan , Herbert Lutz , Bala Raghunath
IPC: C07K1/18 , C07K16/00 , B01D15/08 , C07K1/22 , C07K1/36 , B01D61/14 , C07K16/06 , C07K1/34 , B01D15/12
Abstract: In various embodiments, the present invention provides a process for separating target proteins from non-target proteins in a sample comprising increasing the concentration of the target proteins and non-target proteins in the sample and subsequently delivering the concentrated sample to a chromatography device. In other embodiments, the invention relates to a process for increasing the capacity of a chromatography device for a target protein by delivering a concentrated sample comprising the target protein to a chromatography device.
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公开(公告)号：US10195550B2
公开(公告)日：2019-02-05
申请号：US14839779
申请日：2015-08-28
Applicant: EMD Millipore Corporation
Inventor： Jonathan Steen , Mikhail Kozlov , Herbert Lutz , Joseph Parrella , Elizabeth M. Goodrich , Bala Raghunath
Abstract: A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.
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公开(公告)号：US11691107B2
公开(公告)日：2023-07-04
申请号：US16762688
申请日：2018-11-01
Applicant: EMD Millipore Corporation
Inventor： Elizabeth M. Goodrich , Akshat Gupta , Herbert Lutz
CPC classification number: B01D61/146 , B01D61/18 , B01D61/22 , B01D65/02 , B01D2311/04 , B01D2311/08 , B01D2311/25 , B01D2313/18 , B01D2313/243 , B01D2313/50 , B01D2315/10 , B01D2315/14 , B01D2315/16 , B01D2317/022 , B01D2317/04 , B01D2321/16
Abstract: Processes and systems for filtering a liquid sample are provided. Batches of a liquid sample can be routed to two or more cycling tanks (e.g., first and second cycling tanks). Upon filling a first cycling tank, a first batch of the liquid sample can be routed to a filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the first cycling tank or to a collection vessel. Upon filling a second cycling tank, a second batch of the liquid sample is routed to the filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the second cycling tank or to the collection vessel. The filling and continuous diafiltration of batches of the liquid sample continues to alternate between the two or more cycling tanks until a total product volume is processed.
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公开(公告)号：US11617988B2
公开(公告)日：2023-04-04
申请号：US16207874
申请日：2018-12-03
Applicant: EMD Millipore Corporation
Inventor： Herbert Lutz , Joseph Parrella , Bala Raghunath
Abstract: The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.
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公开(公告)号：US20220402968A1
公开(公告)日：2022-12-22
申请号：US17756096
申请日：2020-12-11
Applicant: EMD Millipore Corporation
Inventor： Herbert Lutz , Kenneth Scott , Michael Bruce , Kristina Cunningham
IPC: C07K1/36 , C12M1/00 , C12N7/00 , C07K1/22 , C07K1/34 , B01D15/38 , B01D15/18 , B01D15/36 , B01D15/32
Abstract: Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to filtration to form a clarified product sample; subjecting said clarified product sample to affinity chromatography to remove impurities; subsequently subjecting said product sample to diafiltration followed by virus filtration and optional concentration. The buffer used during the diafiltration step (and thus in the virus filtration step) is the buffer desired for the final formulation of the product.
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