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11.发明授权Genome editing using targeting endonucleases and single-stranded nucleic acids 有权使用靶向内切核酸酶和单链核酸进行基因组编辑公开(公告)号：US09512444B2
公开(公告)日：2016-12-06
申请号：US13811884
申请日：2011-07-22
Applicant: Fuqiang Chen , Shondra M. Pruett-Miller , Gregory D. Davis
Inventor： Fuqiang Chen , Shondra M. Pruett-Miller , Gregory D. Davis
CPC classification number: C12N15/85 , C12N9/22 , C12N15/907
Abstract: The present invention provides methods and kits for editing specific chromosomal sequences in cells. In particular, targeting endonucleases and single-stranded nucleic acids are used to edit the chromosomal sequence.
Abstract translation: 本发明提供用于编辑细胞中特定染色体序列的方法和试剂盒。 特别地，靶向核酸内切酶和单链核酸用于编辑染色体序列。
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12.发明授权公开(公告)号：US09062303B2
公开(公告)日：2015-06-23
申请号：US12820250
申请日：2010-06-22
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
Abstract translation: 本发明提供了从生物样品中快速和有效地分离小RNA的方法。 特别地，将小RNA从生物样品中的大RNA，DNA，蛋白质和其他大分子中分离和分离。
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13.发明申请公开(公告)号：US20100256351A1
公开(公告)日：2010-10-07
申请号：US12820250
申请日：2010-06-22
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
IPC: C07H21/02
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
Abstract translation: 本发明提供了从生物样品中快速和有效地分离小RNA的方法。 特别地，将小RNA从生物样品中的大RNA，DNA，蛋白质和其他大分子中分离和分离。
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Patent Agency Ranking
14.发明授权Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography 有权使用碳水化合物非离子型去垢剂与二氧化硅色谱法在质粒制备物中还原内毒素的方法公开(公告)号：US07531308B2
公开(公告)日：2009-05-12
申请号：US11108317
申请日：2005-04-18
Applicant: Kevin Bernard Ray , Carol Ann Kreader , Fuqiang Chen , David Eric Cutter
Inventor： Kevin Bernard Ray , Carol Ann Kreader , Fuqiang Chen , David Eric Cutter
CPC classification number: C07H21/04 , C12N15/101
Abstract: The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.
Abstract translation: 本发明提供使用碳水化合物非离子洗涤剂与二氧化硅色谱法还原质粒制备中的内毒素的方法。
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公开(公告)号：US20080026375A1
公开(公告)日：2008-01-31
申请号：US11461273
申请日：2006-07-31
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: B01J45/00 , B01J20/286 , B01J39/26 , B01J41/20 , G01N30/96
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
Abstract translation: 本发明提供带电分离介质，包含分离介质的试剂盒，以及使用该分离介质结合并任选分离生物分子的方法。 带电分离介质和方法利用非共价结合分离介质的聚离子将期望的生物分子结合到分离介质。
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16.发明申请公开(公告)号：US20070202511A1
公开(公告)日：2007-08-30
申请号：US11363982
申请日：2006-02-28
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12N15/1003 , C12Q1/6806 , C12Q2527/125
Abstract: The present invention provides extraction compositions and methods for the rapid and efficient isolation of small RNA molecules from a biological sample. In particular, the extraction compositions, when contacted with a biological sample, releases the small RNA molecules from the other molecules in a biological sample, and the released small RNA molecules may then be isolated.
Abstract translation: 本发明提供了用于从生物样品中快速和有效地分离小RNA分子的提取组合物和方法。 特别地，当与生物样品接触时，提取组合物从生物样品中的其他分子释放小RNA分子，然后可以分离释放的小RNA分子。
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公开(公告)号：US20070087369A1
公开(公告)日：2007-04-19
申请号：US11564540
申请日：2006-11-29
Applicant: Fuqiang Chen , David Cutter
Inventor： Fuqiang Chen , David Cutter
CPC classification number: C12Q1/6806 , C12Q2527/125 , C12Q2523/308 , C12Q2523/113
Abstract: Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.
Abstract translation: 提供用于分离RNA的方法和试剂盒，其能够从生物来源快速制备RNA。 在一个方面，RNA从含有高水平次级代谢物的困难的植物组织和细胞中分离，而不使用有机提取或盐沉淀方法。 该方法采用新的裂解和结合条件以制备不含次级代谢物的RNA。
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公开(公告)号：US20070015165A1
公开(公告)日：2007-01-18
申请号：US11180087
申请日：2005-07-13
Applicant: Fuqiang Chen , David Cutter
Inventor： Fuqiang Chen , David Cutter
CPC classification number: C12Q1/6806 , C12Q2527/125 , C12Q2523/308 , C12Q2523/113
Abstract: Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.
Abstract translation: 提供用于分离RNA的方法和试剂盒，其能够从生物来源快速制备RNA。 在一个方面，RNA从含有高水平次级代谢物的困难的植物组织和细胞中分离，而不使用有机提取或盐沉淀方法。 该方法采用新的裂解和结合条件以制备不含次级代谢物的RNA。
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