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1.发明申请Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography 有权使用碳水化合物非离子型去垢剂与二氧化硅色谱法在质粒制备物中还原内毒素的方法公开(公告)号：US20050245733A1
公开(公告)日：2005-11-03
申请号：US11108317
申请日：2005-04-18
Applicant: Kevin Ray , Carol Kreader , Fuqiang Chen , David Cutter
Inventor： Kevin Ray , Carol Kreader , Fuqiang Chen , David Cutter
CPC classification number: C07H21/04 , C12N15/101
Abstract: The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.
Abstract translation: 本发明提供使用碳水化合物非离子洗涤剂与二氧化硅色谱法还原质粒制备中的内毒素的方法。
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公开(公告)号：US20070087369A1
公开(公告)日：2007-04-19
申请号：US11564540
申请日：2006-11-29
Applicant: Fuqiang Chen , David Cutter
Inventor： Fuqiang Chen , David Cutter
CPC classification number: C12Q1/6806 , C12Q2527/125 , C12Q2523/308 , C12Q2523/113
Abstract: Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.
Abstract translation: 提供用于分离RNA的方法和试剂盒，其能够从生物来源快速制备RNA。 在一个方面，RNA从含有高水平次级代谢物的困难的植物组织和细胞中分离，而不使用有机提取或盐沉淀方法。 该方法采用新的裂解和结合条件以制备不含次级代谢物的RNA。
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公开(公告)号：US20070015165A1
公开(公告)日：2007-01-18
申请号：US11180087
申请日：2005-07-13
Applicant: Fuqiang Chen , David Cutter
Inventor： Fuqiang Chen , David Cutter
CPC classification number: C12Q1/6806 , C12Q2527/125 , C12Q2523/308 , C12Q2523/113
Abstract: Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.
Abstract translation: 提供用于分离RNA的方法和试剂盒，其能够从生物来源快速制备RNA。 在一个方面，RNA从含有高水平次级代谢物的困难的植物组织和细胞中分离，而不使用有机提取或盐沉淀方法。 该方法采用新的裂解和结合条件以制备不含次级代谢物的RNA。
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公开(公告)号：US20100175861A1
公开(公告)日：2010-07-15
申请号：US12731534
申请日：2010-03-25
Applicant: Jiubo Ma , Zhishang Yang , Shizhu Zhao , Shoudu Zhang , Yuexuan Sheng , Lianmin Yang , Zengqiang Wang , Fuqiang Chen , Xinqiang Xu
Inventor： Jiubo Ma , Zhishang Yang , Shizhu Zhao , Shoudu Zhang , Yuexuan Sheng , Lianmin Yang , Zengqiang Wang , Fuqiang Chen , Xinqiang Xu
CPC classification number: F28F1/04 , B23K26/242 , B23K26/244 , F28D1/05366 , F28F2275/067
Abstract: A tube assembly for a beat exchanger is provided having a first U-shaped stainless steel assembly component and a second U-shaped stainless steel assembly component. In addition, the tube assembly has at least one laser-welded joint coupling the first and second U-shaped assembly components together. The laser-welded joint has a weld that extends along substantially an entire length of the tube assembly.
Abstract translation: 提供了一种用于节拍交换器的管组件，其具有第一U形不锈钢组件部件和第二U形不锈钢组件部件。 此外，管组件具有将第一和第二U形组件部件联接在一起的至少一个激光焊接接头。 激光焊接接头具有沿管组件的大致整个长度延伸的焊缝。
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公开(公告)号：US20140273233A1
公开(公告)日：2014-09-18
申请号：US14292212
申请日：2014-05-30
Applicant: Fuqiang Chen , Gregory Davis
Inventor： Fuqiang Chen , Gregory Davis
IPC: C12N15/90
CPC classification number: C12N15/85 , C07K2319/09 , C12N9/22 , C12N15/902
Abstract: The present invention provides methods for modifying chromosomal sequences. In particular, methods are provided for using RNA-guided endonucleases or modified RNA-guided endonucleases to modify targeted chromosomal sequences.
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Patent Agency Ranking
6.发明申请PROCESS FOR THE REDUCTION OF ENDOTOXINS IN A PLASMID PREPARATION USING A CARBOHYDRATE NON-IONIC DETERGENT WITH SILICA CHROMATOGRAPHY 审中-公开使用二氧化硅色谱法制造碳酸盐非离子清除剂，在减少制剂中减少内毒素的方法公开(公告)号：US20090240044A1
公开(公告)日：2009-09-24
申请号：US12416581
申请日：2009-04-01
Applicant: Kevin Bernard Ray , Carol Ann Kreader , Fuqiang Chen , David Eric Cutter
Inventor： Kevin Bernard Ray , Carol Ann Kreader , Fuqiang Chen , David Eric Cutter
CPC classification number: C07H21/04 , C12N15/101
Abstract: The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.
Abstract translation: 本发明提供使用碳水化合物非离子洗涤剂与二氧化硅色谱法还原质粒制备中的内毒素的方法。
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公开(公告)号：US20080026374A1
公开(公告)日：2008-01-31
申请号：US11461263
申请日：2006-07-31
Applicant: Fuqiang Chen , Carol Kreader
Inventor： Fuqiang Chen , Carol Kreader
CPC classification number: C12Q1/6813
Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
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公开(公告)号：US20050164260A1
公开(公告)日：2005-07-28
申请号：US11027150
申请日：2004-12-30
Applicant: Fuqiang Chen
Inventor： Fuqiang Chen
CPC classification number: C12N15/1003 , C12Q1/6806
Abstract: The present invention provides methods, compositions and kits for isolating and purifying at least one nucleic acid directly from a biological sample, or for preparing a biological sample for subsequent isolation and purification of at least one nucleic acid, without removal of the biological sample's cell culture medium or cellular fluid.
Abstract translation: 本发明提供了用于从生物样品直接分离和纯化至少一种核酸的方法，组合物和试剂盒，或用于制备用于随后分离和纯化至少一种核酸的生物样品，而不去除生物样品的细胞培养物 中等或细胞液。
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公开(公告)号：US20140273230A1
公开(公告)日：2014-09-18
申请号：US14213895
申请日：2014-03-14
Applicant: Fuqiang Chen , Gregory Davis
Inventor： Fuqiang Chen , Gregory Davis
IPC: C12N15/85
CPC classification number: C12N15/85 , C07K2319/09 , C12N9/22 , C12N15/902
Abstract: The present invention provides methods for modifying chromosomal sequences. In particular, methods are provided for using RNA-guided endonucleases or modified RNA-guided endonucleases to modify targeted chromosomal sequences.
Abstract translation: 本发明提供了修饰染色体序列的方法。 特别地，提供了使用RNA引导的内切核酸酶或修饰的RNA引导的内切核酸酶来修饰靶向染色体序列的方法。
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10.发明申请GENOME EDITING USING TARGETING ENDONUCLEASES AND SINGLE-STRANDED NUCLEIC ACIDS 有权使用靶向内切酶和单条核酸的基因组编辑公开(公告)号：US20130137180A1
公开(公告)日：2013-05-30
申请号：US13811884
申请日：2011-07-22
Applicant: Fuqiang Chen , Shondra M. Pruett-Miller , Gregory D. Davis
Inventor： Fuqiang Chen , Shondra M. Pruett-Miller , Gregory D. Davis
IPC: C12N15/85
CPC classification number: C12N15/85 , C12N9/22 , C12N15/907
Abstract: The present invention provides methods and kits for editing specific chromosomal sequences in cells. In particular, targeting endonucleases and single-stranded nucleic acids are used to edit the chromosomal sequence.
Abstract translation: 本发明提供用于编辑细胞中特定染色体序列的方法和试剂盒。 特别地，靶向核酸内切酶和单链核酸用于编辑染色体序列。
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