公开(公告)号：US20190083655A1

公开(公告)日：2019-03-21

申请号：US15707074

申请日：2017-09-18

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  John Richard Nelson ,  Wei Gao

IPC: A61K48/00

CPC classification number: A61K48/0066 ,  C12N15/63 ,  C12N15/67 ,  C12Q2531/125

Abstract: A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.

公开(公告)号：US09957478B2

公开(公告)日：2018-05-01

申请号：US15345418

申请日：2016-11-07

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Brian Michael Davis ,  Evelina Roxana Loghin ,  Kenneth Roger Conway ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne

IPC: C12N5/00 ,  B29C37/00 ,  B29C59/02 ,  B29C71/00 ,  B29C70/00 ,  B29K25/00 ,  B29K105/00 ,  B29L31/00

CPC classification number: C12N5/0068 ,  B29C37/0025 ,  B29C59/02 ,  B29C71/0081 ,  B29K2025/06 ,  B29K2105/256 ,  B29K2883/00 ,  B29L2031/712 ,  C12N2501/999 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  C12N2539/10 ,  Y10T156/1039

Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

公开(公告)号：US20170051248A1

公开(公告)日：2017-02-23

申请号：US15345418

申请日：2016-11-07

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Brian Michael Davis ,  Evelina Roxana Loghin ,  Kenneth Roger Conway ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne

IPC: C12N5/00 ,  B29C71/00 ,  B29C37/00 ,  B29C59/02

CPC classification number: C12N5/0068 ,  B29C37/0025 ,  B29C59/02 ,  B29C71/0081 ,  B29K2025/06 ,  B29K2105/256 ,  B29K2883/00 ,  B29L2031/712 ,  C12N2501/999 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  C12N2539/10 ,  Y10T156/1039

Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

Abstract translation: 提供了用于多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体和使用相同载体培养基质细胞的方法。

公开(公告)号：US20140356949A1

公开(公告)日：2014-12-04

申请号：US14461860

申请日：2014-08-18

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Evelina Roxana Loghin ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne ,  Scott Michael Miller

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  Y10T156/1039

Abstract: A carrier for expansion of induced pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing induced pluripotent stem cells using the same carrier are also provided.

Abstract translation: 提供了用于诱导多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体的方法，以及使用相同的载体培养诱导的多能干细胞。

公开(公告)号：US20140093946A1

公开(公告)日：2014-04-03

申请号：US14039323

申请日：2013-09-27

Applicant: General Electric Company

Inventor： Jean-Baptiste Mathieu ,  Brian Michael Davis ,  Vasile Bogdan Neculaes ,  Evelina Roxana Loghin ,  Kenneth Roger Conway

IPC: C12N15/64

CPC classification number: C12N15/64 ,  C12N15/87

Abstract: An embodiment of a system is provided herein, wherein the system allows for the analysis and selection of numerous experimental conditions to optimize transfection efficiency and cell viability. The system is used for magnetic particle based nucleic acid delivery by optimizing various parameters. The system comprises a control module; an incubation module for incubating magnetic nanoparticle and nucleic acid; a transfection module and an analysis module.

Abstract translation: 本文提供了一种系统的实施例，其中所述系统允许分析和选择许多实验条件以优化转染效率和细胞活力。 该系统通过优化各种参数用于基于磁性粒子的核酸递送。 该系统包括控制模块; 用于孵育磁性纳米颗粒和核酸的孵育模块; 转染模块和分析模块。