公开(公告)号：US12195880B2

公开(公告)日：2025-01-14

申请号：US17872042

申请日：2022-07-25

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Shultz Adler ,  Matthew James Spindler ,  Rena Aviva Mizrahi

IPC: C40B50/06 ,  C07K14/725 ,  C07K14/74 ,  C07K16/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6888 ,  C40B40/08

Abstract: Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

公开(公告)号：US11685779B2

公开(公告)日：2023-06-27

申请号：US17386504

申请日：2021-07-27

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Sheila Keating ,  Adam Shultz Adler ,  Michael Asensio ,  Kacy Stadtmiller ,  Emily Benzie ,  Ariel Niedecken ,  Angelica V. Medina-Cucurella ,  Rena Aviva Mizrahi ,  Yoong Wearn Lim

IPC: C07K16/28 ,  A61P31/14 ,  C40B40/10 ,  A61K39/00

CPC classification number: C07K16/2803 ,  A61P31/14 ,  C40B40/10 ,  A61K2039/505 ,  C07K2317/565 ,  C07K2317/92

Abstract: Provided herein are compositions comprising recombinant polyclonal proteins (RPPs) derived from mammalian plasma cells and plasmablasts. Also provided are methods of using the RPPs.

公开(公告)号：US20230070140A1

公开(公告)日：2023-03-09

申请号：US17862101

申请日：2022-07-11

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C40B40/08 ,  C07K16/00 ,  C12N15/10 ,  C07K14/725 ,  C12Q1/6876

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US11591652B2

公开(公告)日：2023-02-28

申请号：US17520915

申请日：2021-11-08

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/6874 ,  C12N15/10 ,  C07K16/00 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6888 ,  C40B50/06

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US20220056522A1

公开(公告)日：2022-02-24

申请号：US17520915

申请日：2021-11-08

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/6874 ,  C12N15/10 ,  C07K16/00 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6888

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US10787706B2

公开(公告)日：2020-09-29

申请号：US16592622

申请日：2019-10-03

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/6874 ,  C12N15/10 ,  C07K16/00 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6888 ,  C40B50/06

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US20200140947A1

公开(公告)日：2020-05-07

申请号：US16592622

申请日：2019-10-03

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/6881

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US10329557B2

公开(公告)日：2019-06-25

申请号：US15920092

申请日：2018-03-13

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Shultz Adler ,  Matthew James Spindler ,  Rena Aviva Mizrahi

IPC: C40B50/06 ,  C12N15/10 ,  C12Q1/6888 ,  C07K16/00 ,  C07K14/725 ,  C07K14/74 ,  C12Q1/6869 ,  C12Q1/6806

Abstract: Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

公开(公告)号：US20170247684A1

公开(公告)日：2017-08-31

申请号：US15598314

申请日：2017-05-18

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C12N15/10

CPC classification number: C12N15/1037 ,  C07K14/7051 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6876 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US20160362683A1

公开(公告)日：2016-12-15

申请号：US15156226

申请日：2016-05-16

Applicant: Gigagen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C12N15/10 ,  C07K16/00 ,  C12Q1/68

CPC classification number: C12N15/1037 ,  C07K14/7051 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6876 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.