摘要:
The present invention provides a fructosylamine oxidase which is obtainable by culturing Fusarium proliferatum, and purifying two types of fructosylamine oxidase (FAO) with different substrate specificities from the culture, and which is useful in the measurement of amadori compounds.
摘要:
The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant. The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.
摘要:
Disclosed is a protein selected from: (1) a protein comprising the amino acid sequence of SEQ ID NO: 1 in the Sequence Listing; (2) a protein comprising the amino acid sequence of SEQ ID NO: 1 in the Sequence Listing with the deletion, addition, insertion and/or substitution of one or more amino acid residues, and having an alkane polyol dehydrogenase activity; or (3) a protein comprising an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 1 in the Sequence Listing, and having an alkane polyol dehydrogenase activity. Also disclosed is a process for producing an alcohol, a ketone, an optically-active alcohol, dihydroxyacetone or a derivative thereof, using the protein.
摘要翻译:公开了选自以下的蛋白质:(1)包含序列表中SEQ ID NO:1的氨基酸序列的蛋白质; (2)包含序列表中SEQ ID NO:1的氨基酸序列的蛋白质,其具有一个或多个氨基酸残基的缺失,添加,插入和/或取代并具有烷烃多元醇脱氢酶活性; 或(3)包含与序列表中SEQ ID NO:1的氨基酸序列具有80%以上同一性并具有烷烃多元醇脱氢酶活性的氨基酸序列的蛋白质。 还公开了使用该蛋白质生产醇,酮,光学活性醇,二羟基丙酮或其衍生物的方法。
摘要:
Disclosed is a protein selected from:(1) a protein comprising the amino acid sequence of SEQ ID NO: 1 in the Sequence Listing; (2) a protein comprising the amino acid sequence of SEQ ID NO: 1 in the Sequence Listing with the deletion, addition, insertion and/or substitution of one or more amino acid residues, and having an alkane polyol dehydrogenase activity; or (3) a protein comprising an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 1 in the Sequence Listing, and having an alkane polyol dehydrogenase activity. Also disclosed is a process for producing an alcohol, a ketone, an optically-active alcohol, dihydroxyacetone or a derivative thereof, using the protein.
摘要翻译:公开了选自以下的蛋白质:(1)包含序列表中SEQ ID NO:1的氨基酸序列的蛋白质; (2)包含序列表中SEQ ID NO:1的氨基酸序列的蛋白质,其具有一个或多个氨基酸残基的缺失,添加,插入和/或取代并具有烷烃多元醇脱氢酶活性; 或(3)包含与序列表中SEQ ID NO:1的氨基酸序列具有80%以上同一性并具有烷烃多元醇脱氢酶活性的氨基酸序列的蛋白质。 还公开了使用该蛋白质生产醇,酮,光学活性醇,二羟基丙酮或其衍生物的方法。
摘要:
The present invention provides a fructosylamine oxidase which is obtainable by culturing Fusarium proliferatum, and purifying two types of fructosylamine oxidase (FAO) with different substrate specificities from the culture, and which is useful in the measurement of amadori compounds.
摘要:
A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.
摘要:
The objective of the present invention is to produce a novel (R)-2,3-butanediol dehydrogenase useful for the production of ketones, alcohols, and particularly optically active vicinal diols. It has been found that Pichia angusta produces novel (R)-2,3-butanediol dehydrogenase that shows the high activity and high stereoselectivity. Further, polynucleotide encoding this (R)-2,3-butanediol dehydrogenase was cloned, and the nucleotide sequence thereof was determined. The expression of the glycerol dehydrogenase was carried out in heterologous microorganisms.
摘要:
The present invention relates to a fructosyl amino acid oxidase that is obtainable from a culture of a strain of the genus Aspergillus. This strain grows in a selective medium containing at least either of fructosyl lysine and fructosyl-N.sup..alpha. -Z-lysine and is capable of producing a fructosyl amino acid oxidase.
摘要:
The present invention provides a recombinant protein which shows fructosyl amino acid oxidase activity, a DNA encoding the same, an expression vector containing the DNA, a transformant transformed by the expression vector, and the method of preparing recombinant fructosyl amino acid oxidase by culturing the resultant transformant, and the recombinant fructosyl amino acid oxidase thus obtained.
摘要:
A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.