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21 条记录 (耗时 0.02 秒)

    Process for preparing acyl-CoA synthetase LCF-18
    4.
    发明授权
    Process for preparing acyl-CoA synthetase LCF-18 失效
    制备酰基辅酶A合成酶LCF-18的方法

    公开(公告)号：US4229538A

    公开(公告)日：1980-10-21

    申请号：US28604

    申请日：1979-04-09

    申请人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani

    发明人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani

    IPC分类号： C12N9/00 ,  C12Q1/25 ,  C12N9/10

    CPC分类号： C12N9/93 ,  C12Q1/25 ,  Y10S435/875 ,  Y10S435/911

    摘要： Acyl-CoA synthetase, having a high activity to C.sub.16 -C.sub.18 long chain fatty acids, is obtained by culturing strains belonging to various genera. As is known, acyl-CoA synthetase, having a strong activity to C.sub.16 -C.sub.18 long chain fatty acids, is generally obtained from liver of rat; however, it has now been discovered that acyl-CoA synthetase can be obtained from microorganisms and this enzyme is called acyl-CoA synthetase LCF-18. By use of acyl-CoA synthetase LCF-18 of the present invention which has a high activity to C.sub.16 -C.sub.18 long chain fatty acids, serum non-esterified fatty acid of human beings can be accurately determined, and it is very useful for diagnosis of diabetes and so forth.

    摘要翻译： 通过培养属于各种属的菌株获得对C16-C18长链脂肪酸具有高活性的酰基辅酶A合成酶。 众所周知，对C16-C18长链脂肪酸具有强烈活性的酰基辅酶A合成酶通常由大鼠肝脏获得; 然而，现在已经发现可以从微生物获得酰基辅酶A合成酶，该酶称为酰基辅酶A合成酶LCF-18。 通过使用对C16-C18长链脂肪酸具有高活性的本发明的酰基-CoA合成酶LCF-18，可以准确测定人的血清非酯化脂肪酸，并且其对于诊断 糖尿病等。

    Process for producing biotin-vitamers
    6.
    发明授权
    Process for producing biotin-vitamers 失效
    生物素维生素生产工艺

    公开(公告)号：US4563426A

    公开(公告)日：1986-01-07

    申请号：US505124

    申请日：1983-06-21

    申请人： Hideaki Yamada ,  Yoshiki Tani ,  Yoshikazu Izumi

    发明人： Hideaki Yamada ,  Yoshiki Tani ,  Yoshikazu Izumi

    IPC分类号： C12P13/00 ,  C12P17/18 ,  C12R1/01 ,  C12R1/07 ,  C12R1/38 ,  C12N1/38

    CPC分类号： C12P17/186

    摘要： In a process which comprises cultivating a micro-organism having the ability to produce a biotin-vitamer in a culture medium in the presence of a biotin-vitamer precursor thereby to produce the biotin-vitamer and accumulate it in the culture medium, the improvement wherein the biotin-vitamer precursor is added to the culture medium after the microbial cells have been grown.

    摘要翻译： 在包括在生物素维生素前体的存在下培养在培养基中产生生物素维生素的能力的微生物的方法，从而产生生物素维生素并将其积累在培养基中，其改进在于 在培养微生物细胞后，将生物素维生素前体加入到培养基中。

    Process for the determination of free fatty acids
    7.
    发明授权
    Process for the determination of free fatty acids 失效
    测定游离脂肪酸的方法

    公开(公告)号：US4360591A

    公开(公告)日：1982-11-23

    申请号：US212225

    申请日：1980-12-02

    申请人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani ,  Kanzo Yamashita

    发明人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani ,  Kanzo Yamashita

    IPC分类号： G01N33/50 ,  C12Q1/25 ,  C12Q1/44 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/48

    CPC分类号： C12Q1/25

    摘要： This invention relates to a process for the determination of free fatty acids comprising a reaction system 1 in which acyl coenzyme A synthetase is allowed to act on the free fatty acids in the presence of adenosine triphosphate and coenzyme A to yield acyl coenzyme A and a reaction system 2 in which acyl coenzyme A oxidase is allowed to act on the acyl coenzyme A to form hydrogen peroxide, followed by the determination thereof, said process being characterized in that myokinase is added in the reaction system 1 to make it go rapidly to completion.

    摘要翻译： 本发明涉及一种测定游离脂肪酸的方法，其包括反应体系1，其中使酰基辅酶A合成酶在三磷酸腺苷和辅酶A存在下作用于游离脂肪酸，得到酰基辅酶A和反应 系统2，其中使酰基辅酶A氧化酶作用于酰基辅酶A以形成过氧化氢，随后进行测定，所述方法的特征在于将肌激蛋白酶加入反应体系1中使其快速完成。