公开(公告)号：US08702941B2

公开(公告)日：2014-04-22

申请号：US12978280

申请日：2010-12-23

申请人： Yusuke Nakayama ,  Satoshi Yonehara

发明人： Yusuke Nakayama ,  Satoshi Yonehara

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44747 ,  G01N27/44791 ,  G01N33/721

摘要： A method for analyzing hemoglobin by electrophoresis, capable of analyzing hemoglobin A1c (HbA1c) and modified hemoglobin with improved accuracy in a shortened analysis time is provided. The method for analyzing hemoglobin by electrophoresis includes performing electrophoresis under conditions in which an acidic substance having two or more carboxyl groups is present in an electrophoresis solution. At least two of the carboxyl groups of the acidic substance each have an acid dissociation constant (pKa) lower than the pH of the electrophoresis solution at the time of analysis.

摘要翻译： 提供了一种通过电泳分析血红蛋白的方法，其能够在缩短的分析时间内以更高的精度分析血红蛋白A1c（HbA1c）和改良的血红蛋白。 通过电泳分析血红蛋白的方法包括在电泳液中存在具有两个以上羧基的酸性物质的条件下进行电泳。 酸性物质的至少两个羧基在分析时各自具有低于电泳溶液pH值的酸解离常数（pKa）。

公开(公告)号：US08021855B2

公开(公告)日：2011-09-20

申请号：US10521234

申请日：2003-04-28

申请人： Satoshi Yonehara ,  Kaori Ishimaru ,  Kaoru Hirai

发明人： Satoshi Yonehara ,  Kaori Ishimaru ,  Kaoru Hirai

IPC分类号： C12Q1/26

CPC分类号： G01N33/723 ,  C12Q1/26 ,  C12Q1/37 ,  G01N2333/906

摘要： A method of assaying a glycated protein in a sample with the use of redox reaction, in which highly reliable measurement can be obtained. A sample containing a glycated protein is treated with protease in the presence of a sulfonic acid compound, so that the glycated protein is degraded. The glycated portion of the resultant glycated protein degradation product is reacted with fructosyl amino acid oxidase, and this redox reaction is measured, thereby determining the amount of glycated protein. Sodium lauryl sulfate can be used as the sulfonic acid compound.

摘要翻译： 使用氧化还原反应测定样品中的糖化蛋白质的方法，其中可以获得高度可靠的测量。 在磺酸化合物存在下，用蛋白酶处理含有糖化蛋白质的样品，使得糖化蛋白质降解。 所得糖化蛋白质降解产物的糖化部分与果糖基氨基酸氧化酶反应，测定该氧化还原反应，由此测定糖化蛋白质的量。 十二烷基硫酸钠可用作磺酸化合物。

公开(公告)号：US07820404B2

公开(公告)日：2010-10-26

申请号：US11919791

申请日：2006-05-02

申请人： Satoshi Yonehara ,  Norio Inamura

发明人： Satoshi Yonehara ,  Norio Inamura

IPC分类号： C12Q1/37

CPC分类号： C12Q1/37 ,  G01N33/6803 ,  G01N33/723

摘要： The present invention provides a method for cleaving a glycated protein to obtain an amino acid or a peptide efficiently with a protease. By treating the glycated protein with the protease in the presence of a compound represented by R—X, the amino acid or the peptide is obtained by the cleavage. The R represents an alkyl compound with a carbon number of 9 or more, and preferably is straight-chain alkyl or straight-chain acyl with a carbon number of 9 to 16, branched-chain alkyl or branched-chain acyl with a carbon number of 10 to 40 and a main-chain carbon number of 9 to 16, or straight-chain alkyl that is substituted by cycloalkyl (a carbon number of the cycloalkyl ranges from 3 to 8, and a carbon number of the straight chain ranges from 4 to 13), where X is a sugar residue. Moreover, the glycated protein is, for example, glycated hemoglobin, and preferably β-chain N-terminal amino acid or a β-chain N-terminal peptide is cleaved by the protease treatment.

摘要翻译： 本发明提供了用蛋白酶有效切割糖基化蛋白质以获得氨基酸或肽的方法。 通过在由R-X表示的化合物存在下用蛋白酶处理糖化蛋白质，通过裂解获得氨基酸或肽。 R表示碳数为9以上的烷基化合物，优选碳数为9〜16的直链烷基或直链酰基，碳数为碳数为9〜16的支链烷基或支链酰基 10〜40，主链碳数为9〜16，或被环烷基取代的直链烷基（环烷基的碳数为3〜8，直链的碳数为4〜 13），其中X是糖残基。 此外，糖化蛋白质是例如糖化血红蛋白，优选通过蛋白酶处理来切割N-末端氨基酸或一个或多个N-末端肽。

公开(公告)号：US20090081718A1

公开(公告)日：2009-03-26

申请号：US11919791

申请日：2006-05-02

申请人： Satoshi Yonehara ,  Norio Inamura

发明人： Satoshi Yonehara ,  Norio Inamura

IPC分类号： C12Q1/37 ,  C12P21/06 ,  C12P13/04 ,  C12Q1/26

CPC分类号： C12Q1/37 ,  G01N33/6803 ,  G01N33/723

摘要： The present invention provides a method for cleaving a glycated protein to obtain an amino acid or a peptide efficiently with a protease. By treating the glycated protein with the protease in the presence of a compound represented by R—X, the amino acid or the peptide is obtained by the cleavage. The R represents an alkyl compound with a carbon number of 9 or more, and preferably is straight-chain alkyl or straight-chain acyl with a carbon number of 9 to 16, branched-chain alkyl or branched-chain acyl with a carbon number of 10 to 40 and a main-chain carbon number of 9 to 16, or straight-chain alkyl that is substituted by cycloalkyl (a carbon number of the cycloalkyl ranges from 3 to 8, and a carbon number of the straight chain ranges from 4 to 13), where X is a sugar residue. Moreover, the glycated protein is, for example, glycated hemoglobin, and preferably β-chain N-terminal amino acid or a β-chain N-terminal peptide is cleaved by the protease treatment.

摘要翻译： 本发明提供了用蛋白酶有效切割糖基化蛋白质以获得氨基酸或肽的方法。 通过在由R-X表示的化合物存在下用蛋白酶处理糖化蛋白质，通过裂解获得氨基酸或肽。 R表示碳数为9以上的烷基化合物，优选碳数为9〜16的直链烷基或直链酰基，碳数为碳数为9〜16的支链烷基或支链酰基 10〜40，主链碳数为9〜16，或被环烷基取代的直链烷基（环烷基的碳数为3〜8，直链的碳数为4〜 13），其中X是糖残基。 此外，糖化蛋白质是例如糖化血红蛋白，优选通过蛋白酶处理来切割β-链N末端氨基酸或β-链N末端肽。

公开(公告)号：US20090053823A1

公开(公告)日：2009-02-26

申请号：US12087833

申请日：2007-01-18

申请人： Satoshi Yonehara ,  Norio Inamura

发明人： Satoshi Yonehara ,  Norio Inamura

IPC分类号： G01N21/78

CPC分类号： C09B69/04 ,  C09B67/0076 ,  G01N31/22 ,  Y10T436/10 ,  Y10T436/17 ,  Y10T436/173845 ,  Y10T436/25 ,  Y10T436/2525

摘要： A liquid reagent in which a methylene blue compound color former is stably stored in a liquid state; and a method of stabilizing a methylene blue compound color former in a liquid state. A methylene blue compound color former is stabilized by causing it to coexist with either a quaternary ammonium compound having a C12 or higher hydrocarbon chain or a salt thereof in a liquid medium. Examples of the methylene blue compound color former include 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino) phenothiazine.

摘要翻译： 其中亚甲蓝化合物成色剂以液态稳定储存的液体试剂; 以及稳定液态的亚甲蓝化合物着色剂的方法。 通过使其与液体介质中具有C12或更高级烃链的季铵化合物或其盐共存，来稳定亚甲基蓝化合物着色剂。 亚甲基蓝化合物显色剂的实例包括10-（羧甲基氨基羰基）-3,7-双（二甲基氨基）吩噻嗪。

公开(公告)号：US07407783B2

公开(公告)日：2008-08-05

申请号：US10528992

申请日：2003-09-16

申请人： Nobuyuki Yoshida ,  Yoshiki Tani ,  Satoshi Yonehara

发明人： Nobuyuki Yoshida ,  Yoshiki Tani ,  Satoshi Yonehara

IPC分类号： C12N9/06 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/00 ,  C12Q1/42 ,  C07K1/00 ,  C07H21/04

CPC分类号： C12N9/0022 ,  C12Q1/26

摘要： The present invention provides a fructosylamine oxidase which is obtainable by culturing Fusarium proliferatum, and purifying two types of fructosylamine oxidase (FAO) with different substrate specificities from the culture, and which is useful in the measurement of amadori compounds.

摘要翻译： 本发明提供了可通过培养镰刀菌（Fusarium proliferatum）获得的果糖胺氧化酶，并从培养物中纯化具有不同底物特异性的两种类型的果糖胺氧化酶（FAO），其可用于测量阿马多利化合物。

公开(公告)号：US07189576B2

公开(公告)日：2007-03-13

申请号：US10384757

申请日：2003-03-11

申请人： Takao Fukuoka ,  Atsuko Katayama ,  Kenji Yamamoto ,  Satoshi Yonehara

发明人： Takao Fukuoka ,  Atsuko Katayama ,  Kenji Yamamoto ,  Satoshi Yonehara

IPC分类号： G01N31/00

CPC分类号： C12Q1/28 ,  G01N21/272 ,  G01N21/78 ,  G01N31/00 ,  G01N31/22 ,  G01N33/525 ,  G01N33/54393 ,  Y10T436/23 ,  Y10T436/255

摘要： A method of measuring an analyte, comprising a step of measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is caused to exist in the reaction system including the formation reaction of the detectable substance, whereby high-sensitivity measurement is made possible, the detectable substance can be stabilized to improve accuracy of the measurement, a rate of a chemical reaction is increased to enable quick measurement, and high-sensitivity measurement is made possible even in a reaction system which forms an insoluble substance. Also, it can be provided an analytical testing piece for measuring an analyte, by measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein the testing piece comprises at least one test portion having a detection portion for detecting the detectable substance and contains a layered inorganic compound at least in the test portion, whereby diffusion and elution of a dyestuff or the like is prevented, more sensitive and accurate simple, analysis is made possible, and easy handling is possible.

摘要翻译： 一种测量分析物的方法，包括通过使用包含可检测物质的形成反应的反应系统来测量可检测物质的步骤，所述反应系统基于样品中包含的分析物的化学反应，其中存在层状无机化合物 在包括可检测物质的形成反应的反应体系中，由于可以进行高灵敏度的测定，可以使可检测物质稳定化，提高测定精度，提高化学反应的速度，能够进行快速测定，高 即使在形成不溶性物质的反应体系中也能进行敏感度测定。 此外，可以提供一种用于测量分析物的分析测试片，通过使用包括可检测物质的形成反应的反应系统，通过测量包含在样品中的分析物的化学反应来测量可检测物质，其中测试片 至少包含至少一个测试部分，其具有用于检测可检测物质的检测部分，并且至少在测试部分中含有层状无机化合物，从而防止染料等的扩散和洗脱，更加灵敏和准确的简单，分析 可能，易于处理是可能的。

公开(公告)号：US06790665B2

公开(公告)日：2004-09-14

申请号：US10380961

申请日：2003-03-20

申请人： Satoshi Yonehara ,  Yuji Yagi

发明人： Satoshi Yonehara ,  Yuji Yagi

IPC分类号： G01N3372

CPC分类号： G01N33/721 ,  G01N21/31 ,  G01N21/78 ,  G01N33/723 ,  Y10S436/904

摘要： A method of determining Hb is provided, by which an amount of Hb can be determined easily and accurately without fear of damage to the environment. Hemoglobin in a sample is denatured with a tetrazolium compound to give denatured hemoglobin, and an amount of an optical change in the sample is measured at an absorption wavelength specific to the denatured hemoglobin. Using the amount of the optical change thus measured, an amount of the hemoglobin in the sample can be determined. The amount of the optical change preferably is measured at a wavelength in a range from 520 to 670 nm. According to this method, an amount of Hb can be determined with high accuracy as shown in FIG. 1.

摘要翻译： 提供一种确定Hb的方法，通过该方法可以容易且准确地确定Hb的量，而不用担心对环境的损害。 样品中的血红蛋白用四唑化合物变性以变性血红蛋白，并且在变性血红蛋白特异性的吸收波长处测量样品中的光学变化量。 使用如此测量的光学变化量，可以确定样品中血红蛋白的量。 光学变化的量优选在520〜670nm的波长下测定。 根据该方法，可以高精度地确定Hb量，如图3所示。 1。

公开(公告)号：US09121821B2

公开(公告)日：2015-09-01

申请号：US12376739

申请日：2007-08-29

申请人： Yoshihide Tanaka ,  Shinichi Wakida ,  Yusuke Nakayama ,  Satoshi Yonehara

发明人： Yoshihide Tanaka ,  Shinichi Wakida ,  Yusuke Nakayama ,  Satoshi Yonehara

IPC分类号： G01N27/447

CPC分类号： G01N27/447 ,  G01N27/44756

摘要： A process for analyzing a sample by a capillary electrophoresis method is provided that allows for high analytic precision and reduction in apparatus size, and can be readily carried out by electrophoresing a complex of a sample and an anionic group-containing compound in the capillary channel, wherein the capillary channel includes an A layer that is coated on an inner wall thereof and a B layer that is coated on the A layer, where the A and B layers are as described.

摘要翻译： 提供了通过毛细管电泳方法分析样品的方法，其允许高分析精度和装置尺寸的降低，并且可以容易地通过在毛细通道中电泳样品和含阴离子基团的化合物的络合物来进行， 其中毛细通道包括涂覆在其内壁上的A层和涂覆在A层上的B层，其中A层和B层如上所述。

公开(公告)号：US08361292B2

公开(公告)日：2013-01-29

申请号：US12514293

申请日：2008-04-23

申请人： Yusuke Nakayama ,  Satoshi Yonehara

发明人： Yusuke Nakayama ,  Satoshi Yonehara

IPC分类号： G01N27/447

CPC分类号： G01N27/44747

摘要： The present invention provides a method for analyzing hemoglobin by capillary electrophoresis, that allows the apparatus to be smaller in size, allows a highly precise analysis to be obtained, and allows the analysis to be performed in a short period of time. The analytical method of the present invention are methods for analyzing hemoglobin by capillary electrophoresis, comprising: a sample-providing step of providing a sample containing hemoglobin; a capillary tube-providing step of providing a capillary tube containing a buffer solution; and an electrophoresis step of carrying out electrophoresis of the sample, by introducing the sample into the buffer solution in the capillary tube, and applying a voltage across both ends of the capillary tube; wherein the electrophoresis is carried out following at least one of modes (A) and (B) below: (A) the electrophoresis is carried out with a surfactant (a) added to the buffer solution, the surfactant (a) being a non-ionic surfactant having an alkyl group as a hydrophobic portion and a sugar as a hydrophilic portion; and (B) the electrophoresis is carried out with a surfactant (b) added to the sample, the surfactant (b) being a betaine-type amphoteric surfactant.

摘要翻译： 本发明提供了一种通过毛细管电泳分析血红蛋白的方法，其允许装置尺寸更小，可以获得高精度分析，并允许在短时间内进行分析。 本发明的分析方法是通过毛细管电泳分析血红蛋白的方法，包括：提供含有血红蛋白的样品的样品提供步骤; 毛细管提供步骤，提供含有缓冲溶液的毛细管; 以及电泳步骤，通过将样品引入毛细管中的缓冲溶液中并在毛细管的两端施加电压，进行样品的电泳; 其中所述电泳按照以下模式（A）和（B）中的至少一种进行：（A）用添加到缓冲溶液中的表面活性剂（a）进行电泳，所述表面活性剂（a） 具有作为疏水部分的烷基和作为亲水部分的糖的离子表面活性剂; （B）用表面活性剂（b）进行电泳，表面活性剂（b）为甜菜碱型两性表面活性剂。