公开(公告)号：US20080248559A1

公开(公告)日：2008-10-09

申请号：US10593183

申请日：2005-03-25

申请人： Hiroko Inomata ,  Rie Iwata

发明人： Hiroko Inomata ,  Rie Iwata

IPC分类号： C12N1/08 ,  C07H1/06 ,  C12M1/00

CPC分类号： C12N15/1017 ,  B01D15/08

摘要： A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm2 of the membrane. And a method for selectively separating and purifying RNA or DNA, which comprises the steps of: (2-a) adsorbing nucleic acid; (2-b) washing by a washing solution; and (2-c) desorbing the nucleic acid from a nucleic acid-adsorbing porous membrane, wherein the washing solution contains a water-soluble organic solvent having a concentration of 50% by weight or less, and does not contain a chaotropic salt.

摘要翻译： 一种用于从含有DNA和RNA的核酸的混合溶液中选择性分离和纯化RNA的方法，其中所述方法包括以下步骤：（1-a）吸附核酸; （1-b）洗涤; （1-c）进行DNA酶处理; （1-d）洗涤; 和（1-e）通过回收溶液从核酸吸附多孔膜解吸RNA，其中在步骤（1-c）中，DNase溶液的总量为每1cm 2 130ul或更少， 2的膜。 以及选择性分离和纯化RNA或DNA的方法，包括以下步骤：（2-a）吸附核酸; （2-b）用洗涤液洗涤; 和（2-c）从核酸吸附多孔膜解吸核酸，其中洗涤液含有浓度为50重量％以下的水溶性有机溶剂，不含有离液序列。

公开(公告)号：US20080113356A1

公开(公告)日：2008-05-15

申请号：US11794426

申请日：2006-02-02

申请人： Tasuku Sasaki ,  Hiroko Inomata ,  Rie Iwata

发明人： Tasuku Sasaki ,  Hiroko Inomata ,  Rie Iwata

IPC分类号： C12Q1/68 ,  C12N1/08 ,  C07H21/04

CPC分类号： C12N15/1006

摘要： A method for separating and purifying a nucleic acid comprising steps of: (1) adding a lysis solution to a biomaterial to prepare a sample solution containing a nucleic acid, and adding a water-soluble organic solvent or a solution containing a water-soluble organic solvent to the sample solution thereby preparing a sample solution containing the water-soluble organic solvent; (2) contacting the sample solution containing the water-soluble organic solvent with a solid phase thereby adsorbing the nucleic acid on the solid phase; (3) contacting a washing solution with the solid phase thereby washing the solid phase in a state where the nucleic acid is adsorbed on the solid phase; and (4) contacting a recovering solution with the solid phase thereby desorbing the nucleic acid from the solid phase, wherein, in the step (1), the water-soluble organic solvent or the solution containing the water-soluble organic solvent is added separately in at least two batches.

摘要翻译： 一种分离和纯化核酸的方法，包括以下步骤：（1）向生物材料中加入裂解溶液以制备含有核酸的样品溶液，并加入水溶性有机溶剂或含有水溶性有机溶剂 溶剂，从而制备含有水溶性有机溶剂的样品溶液; （2）使含有水溶性有机溶剂的样品溶液与固相接触，从而将核酸吸附在固相上; （3）使洗涤液与固相接触，从而在核酸被吸附在固相上的状态下洗涤固相; 和（4）使回收溶液与固相接触，从而从固相中解吸核酸，其中在步骤（1）中，分别加入水溶性有机溶剂或含有水溶性有机溶剂的溶液 至少两批。

公开(公告)号：US07674529B2

公开(公告)日：2010-03-09

申请号：US11311340

申请日：2005-12-20

申请人： Hiroko Inomata

发明人： Hiroko Inomata

IPC分类号： C08L75/00 ,  B32B27/08 ,  B32B27/34

CPC分类号： C08G69/48 ,  C08G18/603 ,  C08G18/8116 ,  Y10T428/31551 ,  Y10T428/31725

摘要： A functional group-introduced polyamide solid phase comprising: a polyamide solid phase having an amido group; and an isocyanate compound having an isocyanate group and a functional group, wherein the functional group of the isocyanate compound is introduced onto a surface of the polyamide solid phase by reacting the amido group of the polyamide solid phase with the isocyanate group of the isocyanate compound.

摘要翻译： 引入官能团的聚酰胺固相，其包含：具有酰胺基的聚酰胺固相; 和具有异氰酸酯基和官能团的异氰酸酯化合物，通过使聚酰胺固相的酰胺基与异氰酸酯化合物的异氰酸酯基反应，将异氰酸酯化合物的官能团引入聚酰胺固相的表面。

公开(公告)号：US20060135707A1

公开(公告)日：2006-06-22

申请号：US11311340

申请日：2005-12-20

申请人： Hiroko Inomata

发明人： Hiroko Inomata

IPC分类号： C08G69/48

CPC分类号： C08G69/48 ,  C08G18/603 ,  C08G18/8116 ,  Y10T428/31551 ,  Y10T428/31725

摘要： A functional group-introduced polyamide solid phase comprising: a polyamide solid phase having an amido group; and an isocyanate compound having an isocyanate group and a functional group, wherein the functional group of the isocyanate compound is introduced onto a surface of the polyamide solid phase by reacting the amido group of the polyamide solid phase with the isocyanate group of the isocyanate compound.

摘要翻译： 引入官能团的聚酰胺固相，其包含：具有酰胺基的聚酰胺固相; 和具有异氰酸酯基和官能团的异氰酸酯化合物，通过使聚酰胺固相的酰胺基与异氰酸酯化合物的异氰酸酯基反应，将异氰酸酯化合物的官能团引入聚酰胺固相的表面。

公开(公告)号：US08357502B2

公开(公告)日：2013-01-22

申请号：US12730437

申请日：2010-03-24

申请人： Mariejoy Jumawid ,  Hiroko Inomata

发明人： Mariejoy Jumawid ,  Hiroko Inomata

IPC分类号： C12Q1/60

CPC分类号： G01N33/92 ,  C12Q1/60

摘要： It is an object of the present invention to provide a method for measuring low density lipoprotein cholesterol (LDL-C) in a body fluid, which is able to selectively measure low density lipoprotein cholesterol in a body fluid without using a low density lipoprotein cholesterol-selective surfactant having a risk of generating endocrine-disrupting chemicals. The present invention provides a method for measuring low density lipoprotein cholesterol (LDL-C) in a body fluid, which comprises measuring low density lipoprotein cholesterol (LDL-C) by using (a) cholesterol esterase and (b) cholesterol oxidase or cholesterol dehydrogenase, in the presence of a polyoxyethylene-polyoxypropylene copolymer and a polyglyceryl ether.

摘要翻译： 本发明的目的是提供一种用于测量体液中的低密度脂蛋白胆固醇（LDL-C）的方法，其能够选择性地测量体液中的低密度脂蛋白胆固醇，而不使用低密度脂蛋白胆固醇 - 具有产生内分泌干扰物质的危险的选择性表面活性剂。 本发明提供一种测定体液中的低密度脂蛋白胆固醇（LDL-C）的方法，其包括使用（a）胆固醇酯酶和（b）胆固醇氧化酶或胆固醇脱氢酶测定低密度脂蛋白胆固醇（LDL-C） 在聚氧乙烯 - 聚氧丙烯共聚物和聚甘油醚存在下进行。

公开(公告)号：US07843003B2

公开(公告)日：2010-11-30

申请号：US11822028

申请日：2007-06-29

申请人： Mitsuhiro Yoshimura ,  Hiroko Inomata

发明人： Mitsuhiro Yoshimura ,  Hiroko Inomata

IPC分类号： H01L29/66

CPC分类号： H01L29/7815 ,  H01L29/0696 ,  H01L29/4232 ,  H01L29/4238 ,  H01L2924/0002 ,  H01L2924/00

摘要： An insulated gate semiconductor device includes a one conductivity type semiconductor layer, a first operation part in a surface of the semiconductor layer and a second operation part in the surface of the semiconductor layer that is smaller in area than the first operation part. A first channel region and a first transistor of an opposite conductivity type are provided in the first operation part and a second channel region and a second transistor of the opposite conductivity type are provided in the second operation part. The first operation part is disposed around the second operation part. Accordingly, design rules for four corner portions can be made uniform and depletion layer spreading in corner portions at a peripheral edge of a channel region of an operation part in application of a reverse voltage is also made approximately uniform. Thus, stable VDSS breakdown voltage characteristics can be obtained.

摘要翻译： 绝缘栅半导体器件包括一个导电型半导体层，半导体层表面中的第一操作部分和半导体层表面中比第一操作部分小的第二操作部分。 在第一操作部分中设置有相反导电类型的第一沟道区和第一晶体管，并且在第二操作部中设置相反导电型的第二沟道区和第二晶体管。 第一操作部被配置在第二操作部的周围。 因此，可以使四个角部的设计规则均匀，并且在施加反向电压的同时，在操作部的沟道区的周缘处的拐角部分扩散的耗尽层也大致均匀。 因此，可以获得稳定的VDSS击穿电压特性。

公开(公告)号：US20090143572A1

公开(公告)日：2009-06-04

申请号：US11995310

申请日：2006-08-30

申请人： Hiroko Inomata ,  Tomoko Mori

发明人： Hiroko Inomata ,  Tomoko Mori

IPC分类号： C07H21/02

CPC分类号： C12N15/1017 ,  C12Q1/6806

摘要： A method for separating and purifying RNA including the steps of passing a sample solution containing a nucleic acid, a washing solution and a recovering solution through a nucleic acid-adsorbing porous membrane to adsorb nucleic acid, adsorbing, washing and recovering, in which the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and the sample solution is obtained by a process, comprising the steps of (I) injecting a test sample containing at least one of blood and leukocyte, and further containing an anticoagulant to a container, (II) adding a hemolytic agent to the container to obtain a leukocyte pallet, (III) adding a nucleic acid-solubilizing reagent to the leukocyte pallet to obtain a mixture solution and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid.

摘要翻译： 一种用于分离和纯化RNA的方法，包括使含有核酸，洗涤液和回收溶液的样品溶液通过核酸吸附多孔膜吸附核酸，吸附，洗涤和回收的步骤，其中核酸 酸吸附多孔膜是能够通过基本上不含离子键的相互作用吸附核酸的多孔膜，并且通过包括以下步骤的方法获得样品溶液：（I）将含有至少一种血液和 （II）向容器中加入溶血剂以获得白细胞托盘，（III）向白细胞托盘中加入核酸增溶试剂以获得混合溶液，和（IV）将白蛋白沉淀物， 向混合溶液中加入水溶性有机溶剂，得到含有核酸的样品溶液。

公开(公告)号：US20080001221A1

公开(公告)日：2008-01-03

申请号：US11822028

申请日：2007-06-29

申请人： Mitsuhiro Yoshimura ,  Hiroko Inomata

发明人： Mitsuhiro Yoshimura ,  Hiroko Inomata

IPC分类号： H01L29/76

CPC分类号： H01L29/7815 ,  H01L29/0696 ,  H01L29/4232 ,  H01L29/4238 ,  H01L2924/0002 ,  H01L2924/00

摘要： An insulated gate semiconductor device includes a one conductivity type semiconductor layer, a first operation part in a surface of the semiconductor layer and a second operation part in the surface of the semiconductor layer that is smaller in area than the first operation part. A first channel region and a first transistor of an opposite conductivity type are provided in the first operation part and a second channel region and a second transistor of the opposite conductivity type are provided in the second operation part. The first operation part is disposed around the second operation part. Accordingly, design rules for four corner portions can be made uniform and depletion layer spreading in corner portions at a peripheral edge of a channel region of an operation part in application of a reverse voltage is also made approximately uniform. Thus, stable VDSS breakdown voltage characteristics can be obtained.

摘要翻译： 绝缘栅半导体器件包括一个导电型半导体层，半导体层表面中的第一操作部分和半导体层表面中比第一操作部分小的第二操作部分。 在第一操作部分中设置有相反导电类型的第一沟道区和第一晶体管，并且在第二操作部中设置相反导电型的第二沟道区和第二晶体管。 第一操作部被配置在第二操作部的周围。 因此，可以使四个角部的设计规则均匀，并且在施加反向电压的同时，在操作部的沟道区的周缘处的拐角部分扩散的耗尽层也大致均匀。 因此，可以获得稳定的VDSS击穿电压特性。

公开(公告)号：US20070275429A1

公开(公告)日：2007-11-29

申请号：US11798141

申请日：2007-05-10

申请人： Yoshihiko Abe ,  Nobuhito Masuda ,  Hiroko Inomata

发明人： Yoshihiko Abe ,  Nobuhito Masuda ,  Hiroko Inomata

IPC分类号： C12Q1/60

CPC分类号： C12Q1/60 ,  G01N33/92

摘要： An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived from Schizophyllum commune or Pseudomonas sp. and cholesterol oxidase derived from Pseudomonas sp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.

摘要翻译： 本发明要达到的目的是提供一种通过使用便宜的材料方便，快速，具体地测量样品中的HDL-C的方法，并提供用于HDL-C检测的试剂盒和干燥分析元件 用于HDL-C检测。 本发明提供了一种测定体液测试样品中的高密度脂蛋白胆固醇（HDL-C）的方法，其中来源于裂殖酵母（Schizophyllum commune）或假单胞菌属（Pseudomonas sp。）的胆固醇酯酶。 和来源于假单胞菌属的胆固醇氧化酶。 用于从HDL-C产生过氧化氢，从而选择性地测量HDL-C。