-
公开(公告)号：US07884201B2
公开(公告)日：2011-02-08
申请号：US11995310
申请日：2006-08-30
申请人： Hiroko Inomata , Tomoko Mori
发明人： Hiroko Inomata , Tomoko Mori
IPC分类号： C07H21/00
CPC分类号： C12N15/1017 , C12Q1/6806
摘要： A method for separating and purifying RNA including the steps of passing a sample solution containing a nucleic acid, a washing solution and a recovering solution through a nucleic acid-adsorbing porous membrane to adsorb nucleic, adsorbing, washing and recovering, in which the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and the sample solution is obtained by a process, comprising the steps of (I) injecting a test sample containing at least one of blood and leukocyte, and further containing an anticoagulant to a container, (II) adding a hemolytic agent to the container to obtain a leukocyte pellet, (III) adding a nucleic acid-solubilizing reagent to the leukocyte pallet to obtain a mixture solution and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid.
摘要翻译： 一种用于分离和纯化RNA的方法，包括使含有核酸，洗涤溶液和回收溶液的样品溶液通过核酸吸附多孔膜以吸附核酸，吸附，洗涤和回收的步骤，其中核酸 吸附多孔膜是能够通过基本上不含离子键的相互作用吸附核酸的多孔膜，并且通过包括以下步骤的方法获得样品溶液：（I）注射含有血液和白细胞中的至少一种的测试样品 （II）向容器中添加溶血剂以获得白细胞颗粒，（III）向白细胞托盘加入核酸增溶剂以获得混合溶液，并且（IV）加入到抗体凝胶中， 向混合溶液中加入水溶性有机溶剂，得到含有核酸的样品溶液。
-
公开(公告)号：US20090143572A1
公开(公告)日：2009-06-04
申请号：US11995310
申请日：2006-08-30
申请人： Hiroko Inomata , Tomoko Mori
发明人： Hiroko Inomata , Tomoko Mori
IPC分类号： C07H21/02
CPC分类号： C12N15/1017 , C12Q1/6806
摘要： A method for separating and purifying RNA including the steps of passing a sample solution containing a nucleic acid, a washing solution and a recovering solution through a nucleic acid-adsorbing porous membrane to adsorb nucleic acid, adsorbing, washing and recovering, in which the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and the sample solution is obtained by a process, comprising the steps of (I) injecting a test sample containing at least one of blood and leukocyte, and further containing an anticoagulant to a container, (II) adding a hemolytic agent to the container to obtain a leukocyte pallet, (III) adding a nucleic acid-solubilizing reagent to the leukocyte pallet to obtain a mixture solution and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid.
摘要翻译： 一种用于分离和纯化RNA的方法，包括使含有核酸，洗涤液和回收溶液的样品溶液通过核酸吸附多孔膜吸附核酸，吸附，洗涤和回收的步骤，其中核酸 酸吸附多孔膜是能够通过基本上不含离子键的相互作用吸附核酸的多孔膜，并且通过包括以下步骤的方法获得样品溶液：（I）将含有至少一种血液和 （II）向容器中加入溶血剂以获得白细胞托盘，（III）向白细胞托盘中加入核酸增溶试剂以获得混合溶液，和（IV）将白蛋白沉淀物， 向混合溶液中加入水溶性有机溶剂，得到含有核酸的样品溶液。
-
公开(公告)号：US20110207160A1
公开(公告)日：2011-08-25
申请号：US13032155
申请日：2011-02-22
申请人： Hiroko Inomata , Mariejoy Jumawid
发明人： Hiroko Inomata , Mariejoy Jumawid
CPC分类号： G01N33/92 , C12Q1/26 , C12Q1/44 , C12Q1/60 , Y10T436/25375
摘要： It is an object of the present invention to provide a method capable of measuring low density lipoprotein cholesterol (LDL) in a body fluid with high selectivity. The present invention provides a method for measuring low density lipoprotein cholesterol (LDL-C) in a body fluid, which comprises treating the body fluid with a polymer compound having an allylamine or diallylamine unit, and measuring the low density lipoprotein cholesterol (LDL-C) using (a) cholesterol esterase and (b) cholesterol oxidase or cholesterol dehydrogenase.
摘要翻译： 本发明的目的是提供能够以高选择性测量体液中的低密度脂蛋白胆固醇（LDL）的方法。 本发明提供一种测定体液中的低密度脂蛋白胆固醇（LDL-C）的方法，其包括用具有烯丙胺或二烯丙基胺单元的高分子化合物处理体液，测定低密度脂蛋白胆固醇（LDL-C ）使用（a）胆固醇酯酶和（b）胆固醇氧化酶或胆固醇脱氢酶。
-
公开(公告)号：US20070009893A1
公开(公告)日：2007-01-11
申请号：US10568101
申请日：2004-09-08
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。
-
公开(公告)号：US08859221B2
公开(公告)日：2014-10-14
申请号：US11798141
申请日：2007-05-10
申请人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
发明人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
摘要： An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived from Schizophyllum commune or Pseudomonas sp. and cholesterol oxidase derived from Pseudomonas sp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.
摘要翻译： 本发明要达到的目的是提供一种通过使用便宜的材料方便，快速，具体地测量样品中的HDL-C的方法，并提供用于HDL-C检测的试剂盒和干燥分析元件 用于HDL-C检测。 本发明提供了一种测定体液测试样品中的高密度脂蛋白胆固醇（HDL-C）的方法，其中来源于裂殖酵母（Schizophyllum commune）或假单胞菌属（Pseudomonas sp。）的胆固醇酯酶。 和来源于假单胞菌属的胆固醇氧化酶。 用于从HDL-C产生过氧化氢，从而选择性地测量HDL-C。
-
6.发明授权公开(公告)号：US08163472B2
公开(公告)日：2012-04-24
申请号：US12410788
申请日：2009-03-25
申请人： Hiroko Inomata
发明人： Hiroko Inomata
IPC分类号： C12Q1/00
CPC分类号： G01N33/521 , C12Q1/28
摘要： It is an object of the present invention to reduce influence of hemolysis in a dry analytical element used for measurement of components in a body fluid sample such as blood. The present invention provides a method for producing a dry analytical element for body fluid component measurement comprising at least a reagent layer containing an H2O2 color developing reagent and a spreading layer provided on the reagent layer, which comprises steps of providing a spreading layer substrate on the reagent layer containing an H2O2 color developing reagent and preparing a spreading layer by coating a low-viscosity solution containing oxidase to the spreading layer substrate and then coating a high-viscosity solution containing other reagent components than oxidase thereto.
摘要翻译： 本发明的目的是减少用于测量体液样品如血液中的组分的干燥分析元件中溶血的影响。 本发明提供一种体液成分测定用干燥分析元件的制造方法，至少包含含有H 2 O 2显色剂的试剂层和设置在试剂层上的扩散层，该方法包括以下步骤： 试剂层，通过将含有氧化酶的低粘度溶液涂布在扩散层基材上，然后涂布含有除氧化酶以外的其他试剂成分的高粘度溶液，制备扩散层。
-
公开(公告)号：US07572578B2
公开(公告)日：2009-08-11
申请号：US10568101
申请日：2004-09-08
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。
-
8.发明授权Method for selectively separating and purifying RNA and method for separating and purifying nucleic acid 有权选择性分离和纯化RNA的方法和分离和纯化核酸的方法公开(公告)号：US07824855B2
公开(公告)日：2010-11-02
申请号：US10593183
申请日：2005-03-25
申请人： Hiroko Inomata , Rie Iwata
发明人： Hiroko Inomata , Rie Iwata
CPC分类号： C12N15/1017 , B01D15/08
摘要： A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm2 of the membrane. And a method for selectively separating and purifying RNA or DNA, which comprises the steps of: (2-a) adsorbing nucleic acid; (2-b) washing by a washing solution; and (2-c) desorbing the nucleic acid from a nucleic acid-adsorbing porous membrane, wherein the washing solution contains a water-soluble organic solvent having a concentration of 50% by weight or less, and does not contain a chaotropic salt.
摘要翻译： 一种用于从含有DNA和RNA的核酸的混合溶液中选择性分离和纯化RNA的方法，其中所述方法包括以下步骤：（1-a）吸附核酸; （1-b）洗涤; （1-c）进行DNA酶处理; （1-d）洗涤; 和（1-e）通过回收溶液从核酸吸附多孔膜解吸RNA，其中在步骤（1-c）中，每1cm 2膜的DNA酶溶液的总量为130μl以下 。 以及选择性分离和纯化RNA或DNA的方法，包括以下步骤：（2-a）吸附核酸; （2-b）用洗涤液洗涤; 和（2-c）从核酸吸附多孔膜解吸所述核酸，其中所述洗涤液含有浓度为50重量％以下的水溶性有机溶剂，并且不含离液序列。
-
9.发明申请Method For Selectively Separating and Purifying Rna and Method For Separating and Purifying Nucleic Acid 有权选择性分离和纯化Rna的方法和分离和纯化核酸的方法公开(公告)号：US20080248559A1
公开(公告)日：2008-10-09
申请号：US10593183
申请日：2005-03-25
申请人： Hiroko Inomata , Rie Iwata
发明人： Hiroko Inomata , Rie Iwata
CPC分类号： C12N15/1017 , B01D15/08
摘要： A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid; (1-b) washing; (1-c) subjecting to a DNase treatment; (1-d) washing; and (1-e) desorbing the RNA from a nucleic acid-adsorbing porous membrane by a recovering solution, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm2 of the membrane. And a method for selectively separating and purifying RNA or DNA, which comprises the steps of: (2-a) adsorbing nucleic acid; (2-b) washing by a washing solution; and (2-c) desorbing the nucleic acid from a nucleic acid-adsorbing porous membrane, wherein the washing solution contains a water-soluble organic solvent having a concentration of 50% by weight or less, and does not contain a chaotropic salt.
摘要翻译： 一种用于从含有DNA和RNA的核酸的混合溶液中选择性分离和纯化RNA的方法，其中所述方法包括以下步骤：（1-a）吸附核酸; （1-b）洗涤; （1-c）进行DNA酶处理; （1-d）洗涤; 和（1-e）通过回收溶液从核酸吸附多孔膜解吸RNA，其中在步骤（1-c）中，DNase溶液的总量为每1cm 2 130ul或更少， 2的膜。 以及选择性分离和纯化RNA或DNA的方法，包括以下步骤：（2-a）吸附核酸; （2-b）用洗涤液洗涤; 和（2-c）从核酸吸附多孔膜解吸核酸，其中洗涤液含有浓度为50重量％以下的水溶性有机溶剂，不含有离液序列。
-
公开(公告)号：US20080113356A1
公开(公告)日：2008-05-15
申请号：US11794426
申请日：2006-02-02
申请人： Tasuku Sasaki , Hiroko Inomata , Rie Iwata
发明人： Tasuku Sasaki , Hiroko Inomata , Rie Iwata
CPC分类号： C12N15/1006
摘要： A method for separating and purifying a nucleic acid comprising steps of: (1) adding a lysis solution to a biomaterial to prepare a sample solution containing a nucleic acid, and adding a water-soluble organic solvent or a solution containing a water-soluble organic solvent to the sample solution thereby preparing a sample solution containing the water-soluble organic solvent; (2) contacting the sample solution containing the water-soluble organic solvent with a solid phase thereby adsorbing the nucleic acid on the solid phase; (3) contacting a washing solution with the solid phase thereby washing the solid phase in a state where the nucleic acid is adsorbed on the solid phase; and (4) contacting a recovering solution with the solid phase thereby desorbing the nucleic acid from the solid phase, wherein, in the step (1), the water-soluble organic solvent or the solution containing the water-soluble organic solvent is added separately in at least two batches.
摘要翻译： 一种分离和纯化核酸的方法，包括以下步骤：（1）向生物材料中加入裂解溶液以制备含有核酸的样品溶液，并加入水溶性有机溶剂或含有水溶性有机溶剂 溶剂，从而制备含有水溶性有机溶剂的样品溶液; （2）使含有水溶性有机溶剂的样品溶液与固相接触，从而将核酸吸附在固相上; （3）使洗涤液与固相接触，从而在核酸被吸附在固相上的状态下洗涤固相; 和（4）使回收溶液与固相接触，从而从固相中解吸核酸，其中在步骤（1）中，分别加入水溶性有机溶剂或含有水溶性有机溶剂的溶液 至少两批。
-
-
-
-
-
-
-
-
-
搜索结果
19 条记录 (耗时 0.023 秒)
