公开(公告)号：US20220380844A1

公开(公告)日：2022-12-01

申请号：US17743969

申请日：2022-05-13

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Steven Modiano ,  Silke Musa ,  Lewis Kraft ,  Randall Smith ,  Sara Bakhshi ,  Brian Mather ,  Wayne George ,  Maxwell Zimmerley

IPC: C12Q1/6874 ,  B01L3/00

Abstract: In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

公开(公告)号：US10900077B2

公开(公告)日：2021-01-26

申请号：US16405679

申请日：2019-05-07

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US10883937B2

公开(公告)日：2021-01-05

申请号：US16778935

申请日：2020-01-31

Applicant: Illumina, Inc.

Inventor： Tracy H. Fung ,  Poorya Sabounchi ,  Bernard Hirschbein ,  Joseph Pinto ,  Tarun Khurana ,  Randall Smith ,  Wenyi Feng

IPC: G01N21/64 ,  G01N33/53

Abstract: There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

公开(公告)号：US10551317B2

公开(公告)日：2020-02-04

申请号：US16206564

申请日：2018-11-30

Applicant: Illumina, Inc.

Inventor： Tracy H. Fung ,  Poorya Sabounchi ,  Bernard Hirschbein ,  Joseph Pinto ,  Tarun Khurana ,  Randall Smith ,  Wenyi Feng

IPC: G01N21/64 ,  G01N33/53

Abstract: There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

公开(公告)号：US09453258B2

公开(公告)日：2016-09-27

申请号：US13624200

申请日：2012-09-21

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2525/113 ,  C12Q2535/122 ,  C12Q2563/107

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Abstract translation: 本公开提供了用于检测样品中多个不同核苷酸的方法和系统。 具体地，本公开提供了使用比检测到的核苷酸数目少于检测到的核苷酸数目和/或比检测到的核苷酸数目更少的成像事件来检测样品中的多个不同核苷酸。

公开(公告)号：US20250084477A1

公开(公告)日：2025-03-13

申请号：US18575479

申请日：2022-11-01

Applicant: ILLUMINA, INC.

Inventor： Esteban Armijo ,  Jonathan Mark Boutell ,  Jeffrey Brodin ,  Eli M. Carrami ,  Maria Candelaria Rogert Bacigalupo ,  Randall Smith ,  Mathieu Lessard-Viger ,  Ludovic Vincent

IPC: C12Q1/6876

Abstract: An example primer set includes first and second nuclease resistant primers. The first nuclease resistant primer includes a first sequence; a first cleavage site attached at a 3′ end of the first sequence; and a first nuclease resistant modification incorporated between the first sequence and the first cleavage site. The second nuclease resistant primer includes a second sequence that is different from the first sequence; a second nuclease resistant modification incorporated at a 3′ end of the second sequence; and a second cleavage site attached between the second sequence and the second nuclease resistant modification. The second cleavage site is different from the first cleavage site.

公开(公告)号：US11827932B2

公开(公告)日：2023-11-28

申请号：US17153309

申请日：2021-01-20

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6869

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2535/122 ,  C12Q2563/107

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US11333604B2

公开(公告)日：2022-05-17

申请号：US16948524

申请日：2020-09-22

Applicant: Illumina, Inc.

Inventor： Tracy H. Fung ,  Poorya Sabounchi ,  Bernard Hirschbein ,  Joseph Pinto ,  Tarun Khurana ,  Randall Smith ,  Wenyi Feng

IPC: G01N21/64 ,  G01N33/53

Abstract: There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

公开(公告)号：US20220090179A1

公开(公告)日：2022-03-24

申请号：US17472038

申请日：2021-09-10

Applicant: ILLUMINA, INC.

Inventor： Jie Mao ,  Randall Smith

IPC: C12Q1/6834 ,  G01N21/64 ,  C12Q1/6816

Abstract: A method for detecting an abasic site is provided. The method may include flowing a solution over a substrate having a plurality of oligonucleotides coupled thereto. At least one of the oligonucleotides includes an abasic site. The solution may include a fluorophore coupled to a reactive group. The method may include reacting the reactive group with the abasic site to couple the fluorophore to the abasic site; and detecting the abasic site using fluorescence from the fluorophore.

公开(公告)号：US20200340046A1

公开(公告)日：2020-10-29

申请号：US16868308

申请日：2020-05-06

Applicant: ILLUMINA, INC.

Inventor： Peyton Shieh ,  John M. Beierle ,  Michael S. Graige ,  Alexander Fuhrmann ,  Randall Smith ,  Wei Wei ,  Naiqian Zhan

IPC: C12Q1/6837

Abstract: An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.