公开(公告)号：US20220267812A1

公开(公告)日：2022-08-25

申请号：US17676720

申请日：2022-02-21

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Daniel Held ,  Eric Abbate ,  Katherine Noon

IPC: C12P7/10 ,  C12N15/10 ,  C12N1/20 ,  C12N15/70 ,  C12N9/22 ,  C12N15/11 ,  C12N15/52 ,  C10L1/02

Abstract: The present disclosure provides multiplexed engineered cells, automated multi-module instruments and methods of producing biofuel producing cells for enhanced production of biofuels. This platform empowers users to design genetic variant libraries of insertions, swaps, and/or deletions, that can be intentionally or randomly positioned across the entire genome to generate engineered cell populations with improved properties for several common biofuel applications including, but not limited to, improved tolerance to biomass inhibitors, increased thermo-tolerance, increased ethanol production and/or tolerance, expanded carbon utilization abilities, and increased utilization of heterologous proteins or pathways.

公开(公告)号：US20220195423A1

公开(公告)日：2022-06-23

申请号：US17690737

申请日：2022-03-09

Applicant: Inscripta, Inc.

Inventor： Daniel Held

IPC: C12N15/10 ,  C12N15/63 ,  C12N15/11

Abstract: The present disclosure relates to compositions, methods, modules and automated integrated instrumentation for multiplex delivery of “landing pad” edits into the genomes of a population of live cells. The landing pads then may be leveraged to insert very large DNA sequences into the genomes of the population of live cells.

公开(公告)号：US20220145277A1

公开(公告)日：2022-05-12

申请号：US17522399

申请日：2021-11-09

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Tian Tian ,  Daniel Held

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.

公开(公告)号：US20220106708A1

公开(公告)日：2022-04-07

申请号：US17554373

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N15/10 ,  C12N15/85 ,  C40B30/04 ,  C07K14/74 ,  C07K16/28 ,  C12N15/81 ,  C40B20/04 ,  C40B70/00 ,  C12N9/22 ,  C40B40/02 ,  C40B40/10 ,  C12N15/90

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11332850B2

公开(公告)日：2022-05-17

申请号：US17554373

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C40B20/04 ,  C07K16/28 ,  C40B40/02 ,  C12N15/90 ,  C12N15/10 ,  C12N15/85 ,  C40B30/04 ,  C12N9/22 ,  C12N15/81 ,  C40B70/00 ,  C07K14/74 ,  C40B40/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11299731B1

公开(公告)日：2022-04-12

申请号：US17475267

申请日：2021-09-14

Applicant: Inscripta, Inc.

Inventor： Daniel Held

IPC: C12N15/10 ,  C12N15/63 ,  C12N15/11

Abstract: The present disclosure relates to compositions, methods, modules and automated integrated instrumentation for multiplex delivery of “landing pad” edits into the genomes of a population of live cells. The landing pads then may be leveraged to insert very large DNA sequences into the genomes of the population of live cells.

公开(公告)号：US20220090061A1

公开(公告)日：2022-03-24

申请号：US17475267

申请日：2021-09-14

Applicant: Inscripta, Inc.

Inventor： Daniel Held

IPC: C12N15/10 ,  C12N15/11 ,  C12N15/63

Abstract: The present disclosure relates to compositions, methods, modules and automated integrated instrumentation for multiplex delivery of “landing pad” edits into the genomes of a population of live cells. The landing pads then may be leveraged to insert very large DNA sequences into the genomes of the population of live cells.

公开(公告)号：US20210363481A1

公开(公告)日：2021-11-25

申请号：US17319006

申请日：2021-05-12

Applicant: Inscripta, Inc.

Inventor： Eric Abbate ,  Katherine Krouse ,  Richard Fox ,  Daniel Held ,  Michael Clay ,  Nandini Krishnamurthy

IPC: C12N1/20 ,  C12N15/70

Abstract: The present disclosure relates to various different types of mutations in the thrA gene in E. coli leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US20210246574A1

公开(公告)日：2021-08-12

申请号：US17232046

申请日：2021-04-15

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US20210155894A1

公开(公告)日：2021-05-27

申请号：US17159137

申请日：2021-01-26

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held ,  Eric Abbate ,  Michael Clay ,  Katherine Krouse ,  Nandini Krishnamurthy ,  Krishna Yerramsetty

IPC: C12N1/20 ,  C07K14/245

Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.