公开(公告)号：US20210395727A1

公开(公告)日：2021-12-23

申请号：US17463956

申请日：2021-09-01

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani ,  Patrick Westfall

IPC: C12N15/10

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20210269784A1

公开(公告)日：2021-09-02

申请号：US17319022

申请日：2021-05-12

Applicant: Inscripta, Inc.

Inventor： Skylar Stefani ,  Tian Tian ,  Miles Gander

IPC: C12N9/22 ,  C12N15/85 ,  C12Q1/44

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US10815467B2

公开(公告)日：2020-10-27

申请号：US16827639

申请日：2020-03-23

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani

IPC: C12N9/22 ,  C12N15/85 ,  C12Q1/44

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20200308562A1

公开(公告)日：2020-10-01

申请号：US16827639

申请日：2020-03-23

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani

IPC: C12N9/22 ,  C12Q1/44 ,  C12N15/85

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US11746347B2

公开(公告)日：2023-09-05

申请号：US17679203

申请日：2022-02-24

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani ,  Patrick Westfall

IPC: C12N15/10

CPC classification number: C12N15/1037

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US11512297B2

公开(公告)日：2022-11-29

申请号：US17522399

申请日：2021-11-09

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Tian Tian ,  Daniel Held

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.

公开(公告)号：US20220056428A1

公开(公告)日：2022-02-24

申请号：US17518556

申请日：2021-11-03

Applicant: Inscripta, Inc.

Inventor： Skylar Stefani ,  Tian Tian ,  Miles Gander

IPC: C12N9/22 ,  C12N15/85 ,  C12Q1/44

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20220025359A1

公开(公告)日：2022-01-27

申请号：US17300518

申请日：2021-07-27

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Clint Davis ,  Charles Johnson ,  Andrew Garst

IPC: C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N9/22

Abstract: The present disclosure provides compositions of matter, methods, modules and automated multi-module instrumentation for performing editing of live cells followed by curing of editing and engine vectors from prior rounds of editing, followed by curing of the curing vector.

公开(公告)号：US11203762B2

公开(公告)日：2021-12-21

申请号：US16952024

申请日：2020-11-18

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

公开(公告)号：US11053507B2

公开(公告)日：2021-07-06

申请号：US17072023

申请日：2020-10-15

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Charles Johnson ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.