Methods for reducing enzyme consumption in second generation bioethanol fermentation in the prescence of lignin
    12.
    发明授权
    Methods for reducing enzyme consumption in second generation bioethanol fermentation in the prescence of lignin 有权
    第二代生物乙醇发酵中木质素测定中消耗酶消耗的方法

    公开(公告)号:US07972826B2

    公开(公告)日:2011-07-05

    申请号:US12863601

    申请日:2009-02-02

    Abstract: Provided are methods of liquefaction, saccharification and fermentation of pre-treated lignocellulosic biomass in production of bioethanol. Addition of PEG or surfactant is effective to enhance cellulase hydrolysis yields at high dry matter (>20%) at surprisingly low concentrations compared with comparable results in low dry matter hydrolysis. This effect is most pronounced at comparatively low cellulase loading

    Abstract translation: 提供在生物乙醇生产中预处理的木质纤维素生物质的液化,糖化和发酵的方法。 与低干物质水解中可比较的结果相比,添加PEG或表面活性剂有助于在高干物质(> 20%)下以令人惊讶的低浓度提高纤维素酶水解产率。 在相对较低的纤维素酶负载量<7 FPU(g DM)-1下,这种作用最显着。 在高干物质含量下,通过加入少量的PEG或表面活性剂可以减少纤维素酶消耗而不损失水解产率。

    Methods for reducing enzyme consumption in second generation bioethanol fermentation in the presence of lignin.
    13.
    发明申请
    Methods for reducing enzyme consumption in second generation bioethanol fermentation in the presence of lignin. 有权
    在木质素存在下减少第二代生物乙醇发酵中酶消耗的方法。

    公开(公告)号:US20100291650A1

    公开(公告)日:2010-11-18

    申请号:US12863601

    申请日:2009-02-02

    Abstract: Provided are methods of liquefaction, saccharification and fermentation of pre-treated lignocellulosic biomass in production of bioethanol. Addition of PEG or surfactant is effective to enhance cellulase hydrolysis yields at high dry matter (>20%) at surprisingly low concentrations compared with comparable results in low dry matter hydrolysis. This effect is most pronounced at comparatively low cellulase loading

    Abstract translation: 提供在生物乙醇生产中预处理的木质纤维素生物质的液化,糖化和发酵的方法。 与低干物质水解中可比较的结果相比,添加PEG或表面活性剂有助于在高干物质(> 20%)下以令人惊讶的低浓度提高纤维素酶水解产率。 在相对较低的纤维素酶负载量<7 FPU(g DM)-1下,这种作用最显着。 在高干物质含量下,通过加入少量的PEG或表面活性剂可以减少纤维素酶消耗而不损失水解产率。

    Non-sterile fermentation of bioethanol
    17.
    发明申请
    Non-sterile fermentation of bioethanol 失效
    生物乙醇的无菌发酵

    公开(公告)号:US20120122174A1

    公开(公告)日:2012-05-17

    申请号:US13356664

    申请日:2012-01-24

    Applicant: Jan Larsen

    Inventor: Jan Larsen

    Abstract: A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations.

    Abstract translation: 存在一定范围的浓度,其中衍生自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵酵母。 通过优化发酵抑制剂的含量落在此范围内,木质纤维素生物质的酵母发酵可以在非无菌条件下进行,乙醇产量与在无菌条件下达到的相似。 优化的抑制剂水平可以通过在预处理期间和之后控制木质纤维素生物质的水/生物量比来实现,例如通过用预定量的淡水或再循环的处理溶液洗涤先前预处理的木质纤维素生物质的纤维部分。 来自木质纤维素生物质预处理的液体馏分或处理溶液的粗提取物也可为第一代淀粉发酵提供有效的抗物质处理。

    Non-sterile fermentation of bioethanol.
    18.
    发明申请
    Non-sterile fermentation of bioethanol. 失效
    生物乙醇的无菌发酵。

    公开(公告)号:US20110171708A1

    公开(公告)日:2011-07-14

    申请号:US13074011

    申请日:2011-03-29

    Applicant: Jan Larsen

    Inventor: Jan Larsen

    Abstract: A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-bacterial treatment for first generation starch fermentations.

    Abstract translation: 存在一定范围的浓度,其中衍生自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵酵母。 通过优化发酵抑制剂的含量落在此范围内,木质纤维素生物质的酵母发酵可以在非无菌条件下进行,乙醇产量与在无菌条件下达到的相似。 优化的抑制剂水平可以通过在预处理期间和之后控制木质纤维素生物质的水/生物量比来实现,例如通过用预定量的淡水或再循环的处理溶液洗涤先前预处理的木质纤维素生物质的纤维部分。 来自木质纤维素生物质预处理的液体馏分或处理溶液的粗提取物也可为第一代淀粉发酵提供有效的抗菌处理。

    Processing lignocellulosic biomass to fixed, high levels of dry matter content.
    19.
    发明申请
    Processing lignocellulosic biomass to fixed, high levels of dry matter content. 审中-公开
    加工木质纤维素生物质固定,含水量高。

    公开(公告)号:US20110027852A1

    公开(公告)日:2011-02-03

    申请号:US12935587

    申请日:2009-04-14

    Applicant: Jan Larsen

    Inventor: Jan Larsen

    CPC classification number: C12P19/00 Y02E50/16

    Abstract: The invention relates in general to methods of processing lignocellulosic biomass and to methods of pre-treatment of lignocellulosic pretreated with steam biomass. In particular, the invention provides methods which fix moisture levels in lignocellulosic biomass to levels near the inherent water holding capacity of the material.

    Abstract translation: 本发明一般涉及木质纤维素生物质的处理方法和预处理用蒸汽生物质预处理的木质纤维素的方法。 特别地,本发明提供了将木质纤维素生物质中的水分含量固定到接近材料的固有含水量的水平的方法。

    Enzymatic hydrolysis of biomasses having a high dry matter (DM) content
    20.
    发明授权
    Enzymatic hydrolysis of biomasses having a high dry matter (DM) content 有权
    具有高干物质(DM)含量的生物质的酶水解

    公开(公告)号:US07842490B2

    公开(公告)日:2010-11-30

    申请号:US11720079

    申请日:2005-11-07

    CPC classification number: C12P19/12 C12P7/10 C12P19/14 Y02E50/16 Y02E50/343

    Abstract: The present invention relates to a process for liquefaction and saccharification of polysaccharide containing biomasses, having a relatively high dry matter content. The present invention combines enzymatic hydrolysis with a type of mixing relying on the principle of gravity ensuring that the biomasses are subjected to mechanical forces, primarily shear and tear forces. Furthermore, the present invention relates to the further utilization of such processed biomasses, e.g. for subsequent fermentation into bio-ethanol, bio-gas, specialty carbohydrates for food and fees as well as carbon feedstock for processing into plastics and chemicals.

    Abstract translation: 本发明涉及具有相对高的干物质含量的含多糖的生物质的液化和糖化方法。 本发明根据重力原理将酶水解与一种混合相结合,确保生物质受到机械力,主要是剪切力和撕裂力。 此外,本发明涉及进一步利用这种经处理的生物质,例如, 用于随后发酵成生物乙醇,生物气体,特殊碳水化合物用于食品和费用以及用于加工成塑料和化学品的碳原料。

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