公开(公告)号：US06827840B2

公开(公告)日：2004-12-07

申请号：US10170903

申请日：2002-06-13

申请人： Kalle Levon ,  Bin Yu ,  Yanxiu Zhou

发明人： Kalle Levon ,  Bin Yu ,  Yanxiu Zhou

IPC分类号： G01N27327

CPC分类号： G01N27/3335

摘要： Enantiomeric resolution is realized by combining an electrochemical method with ligand exchange (LE) in a novel electrochemical method named chiral ligand exchange potentiometry. Chiral selector ligands preferentially recognize certain enantiomers and undergo ligand exchange with the enantiomeric labile coordination complexes to form diastereoisomeric complexes. These complexes can form in solution and be recognized by an unmodified electrode, or they can be immobilized on the surface of a modified electrode (chiral sensor) incorporated with the chiral selector ligand by polysiloxane monolayer immobilization (PMI). Considerable stereoselectivity occurs in the formation of these diastereoisomeric complexes, and their net charges (Nernst factors) are different, thus enabling enantiomers to be distinguished by potentiometric electrodes without any pre-separation processes.

摘要翻译： 通过将电化学方法与配体交换（LE）结合在一种称为手性配体交换电位法的新型电化学方法中来实现对映体拆分。 手性选择配体优先识别某些对映异构体并与对映异构体不稳定配位络合物进行配体交换以形成非对映异构体复合物。 这些配合物可以在溶液中形成并被未改性的电极识别，或者它们可以通过聚硅氧烷单层固定化（PMI）固定在掺入手性选择配体的改性电极（手性传感器）的表面上。 在形成这些非对映异构体复合物时会产生相当大的立体选择性，它们的净电荷（能斯特因子）是不同的，因此能够通过电位电极区分对映异构体，而无需任何预分离过程。

公开(公告)号：US08679806B2

公开(公告)日：2014-03-25

申请号：US12176942

申请日：2008-07-21

申请人： Kalle Levon ,  Bin Yu ,  Yanxiu Zhou

发明人： Kalle Levon ,  Bin Yu ,  Yanxiu Zhou

IPC分类号： C12N13/00 ,  C12N11/00 ,  G01N33/53 ,  C12Q1/00

CPC分类号： C12Q1/00 ,  C07K9/00 ,  C07K14/32 ,  C07K17/00 ,  C12Q1/003 ,  G01N33/53 ,  G01N33/54353 ,  G01N33/5438 ,  G01N33/54393 ,  G01N33/552

摘要： A real-time method employing a portable peptide-containing potentiometric biosensor, can directly detect and/or quantify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B. subtilis spores in a concentration range of 0.08-7.3×104 CFU/ml. The sensing method exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10−5. The biosensor method not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensing method can distinguish a Bacillus subtilis 1A700 from other B. subtilis strain. Assay time may be as low as about 5 minutes for a single test. Rapid identification of B. anthracis Sterne and B. anthracis ΔAmes was also provided.

摘要翻译： 采用便携式含肽电位生物传感器的实时方法可以直接检测和/或定量细菌孢子。 用于特异性识别枯草芽孢杆菌和炭疽芽孢杆菌的两种肽可以通过聚硅氧烷单层固定（PMI）技术来固定。 传感器通过检测例如浓度范围为0.08-7.3×104CFU / ml的枯草芽孢杆菌孢子将生物识别事件转化为潜在的变化。 该感测方法在其他种类的孢子上表现出对枯草芽孢杆菌孢子的高度选择性识别性能。 其他类型孢子传感器的选择性系数在0-1.0×10-5的范围内。 生物传感器方法不仅具有区分枯草芽孢杆菌和苏云金芽孢杆菌（Thur。）Kurstaki孢子的混合物中的枯草芽孢杆菌孢子的特异性，而且可以区分活枯枯病芽孢杆菌孢子和死枯病芽孢杆菌孢子。 此外，感测方法可以将枯草芽孢杆菌1A700与其他枯草芽孢杆菌菌株区分开。 单次测试的测定时间可能低至约5分钟。 还提供了炭疽杆菌和炭疽芽孢杆菌的快速鉴定。

公开(公告)号：US07943067B2

公开(公告)日：2011-05-17

申请号：US10218554

申请日：2002-08-14

申请人： Sergey Kazakov ,  Marian Kaholek ,  Kalle Levon

发明人： Sergey Kazakov ,  Marian Kaholek ,  Kalle Levon

IPC分类号： B01J13/02 ,  C08J3/28 ,  C08F2/50

CPC分类号： A61K9/5138 ,  A61K9/127 ,  A61K9/1273 ,  B01J13/02 ,  B82Y5/00 ,  C08J3/075 ,  Y10T428/2982 ,  Y10T428/2984 ,  Y10T428/2985 ,  Y10T428/2987 ,  Y10T428/2989 ,  Y10T428/2991

摘要： The present invention includes a method for preparing polymer hydrogel spherical particles on a nanometer scale (nanogels). The method includes encapsulating hydrogel-forming components into liposomes, diluting the large unilamellar liposomes suspension to prevent polymerization outside the liposomes, and polymerizing the encapsulated hydrogel-forming components. The lipid bilayer may be solubilized with detergent. The phospholipid and detergent molecules and their micelles may then be removed by dialysis. The resulting nanogels may then be dried by evaporation in a temperature gradient. Poly(acrylamide), poly(N-isopropylacrylamide), and poly(N-isopropylacrylamide-co-1-vinylimidazole) hydrogel particles with a diameter from 30 to 300 nm were detected and characterized by dynamic light scattering technique. The solvent, temperature, pH, and ionic sensitivities of the nanogels were studied.

摘要翻译： 本发明包括一种制备纳米尺度聚合物水凝胶球形颗粒的方法（纳米凝胶）。 该方法包括将水凝胶形成成分包封在脂质体中，稀释大的单层脂质体悬浮液以防止在脂质体外聚合，并聚合包封的水凝胶形成成分。 脂质双层可以用洗涤剂溶解。 然后可以通过透析除去磷脂和洗涤剂分子及其胶束。 然后可以通过在温度梯度中蒸发来干燥所得的纳米凝胶。 检测直径为30〜300nm的聚（丙烯酰胺），聚（N-异丙基丙烯酰胺）和聚（N-异丙基丙烯酰胺 - 共-1-乙烯基咪唑）水凝胶颗粒，并用动态光散射技术表征。 研究了纳米凝胶的溶剂，温度，pH和离子敏感性。

公开(公告)号：US07618565B2

公开(公告)日：2009-11-17

申请号：US10218553

申请日：2002-08-14

申请人： Sergey Kazakov ,  Marian Kaholek ,  Kalle Levon

发明人： Sergey Kazakov ,  Marian Kaholek ,  Kalle Levon

IPC分类号： C08J3/28 ,  C08F2/50 ,  B01J13/02 ,  B01J13/14 ,  B01J13/20

CPC分类号： A61K9/5138 ,  A61K9/127 ,  A61K9/1273 ,  B01J13/02 ,  B82Y5/00 ,  C08J3/075 ,  Y10T428/2982 ,  Y10T428/2984 ,  Y10T428/2985 ,  Y10T428/2987 ,  Y10T428/2989 ,  Y10T428/2991

摘要： Lipobeads (liposome-encapsulated hydrogels) combine properties of hydrogels and liposomes to create systems that are sensitive to environmental conditions and respond to changes in those conditions in a fast time scale. Lipobeads may be produced by polymerizing anchored or unanchored hydrogels within liposomes or by mixing anchored or unanchored hydrogels with liposomes. Giant lipobeads may be produced by shrinking unanchored nanogels in lipobeads and fusing the resulting lipobead aggregates, long-term aging of anchored or unanchored lipobeads, or mixing anchored or unanchored aggregated nanogels with liposomes. Poly(acrylamide), poly(N-isopropylacrylamide), and poly(N-isopropylacrylamide-co-1-vinylimidazole) lipobeads were produced and characterized.

摘要翻译： Lipobeads（脂质体包封的水凝胶）结合水凝胶和脂质体的特性，以创建对环境条件敏感的系统，并以快速时间尺度响应这些状况的变化。 脂质体可以通过在脂质体内聚合锚定或未锚定的水凝胶或通过将锚定或未锚定的水凝胶与脂质体混合来制备。 巨型脂蛋白可以通过在脂珠体中收缩未溶解的纳米凝胶并使所得脂质体聚集体缩短，锚定或未锚定脂肪细胞的长期老化或将锚定或未锚定的聚集的纳米凝胶与脂质体混合而产生。 制备聚（丙烯酰胺），聚（N-异丙基丙烯酰胺）和聚（N-异丙基丙烯酰胺 - 共-1-乙烯基咪唑）脂珠和表征。

公开(公告)号：US20090114344A1

公开(公告)日：2009-05-07

申请号：US11666567

申请日：2005-11-01

申请人： Victor Barinov ,  Robert Dabrowski ,  Kalle Levon

发明人： Victor Barinov ,  Robert Dabrowski ,  Kalle Levon

IPC分类号： B32B38/00

CPC分类号： B32B43/006 ,  B32B37/12 ,  B32B38/00 ,  B32B2037/1246 ,  B32B2038/0052 ,  C09J5/00 ,  C09J2205/302 ,  Y10T29/49755 ,  Y10T29/49821 ,  Y10T156/11 ,  Y10T156/1158 ,  Y10T156/19

摘要： The invention concerns a method for controlling the adhesive bond between an electric-field responsive material and a substrate, which method comprises using an electric field to control the strength and integrity of the adhesive bond. The method may be used, for example, for removing or delaminating a material, which material is responsive to an electric field, from a substrate. The invention further comprises elimination of the application of the electric field and application of the previously removed electric-field-responsive material to the substrate, thereby resuming the strength and integrity of the adhesive bond. The invention includes apparatus for use in the bond controlling process.

摘要翻译： 本发明涉及一种用于控制电场响应材料和基底之间的粘合剂结合的方法，该方法包括使用电场来控制粘合剂粘结的强度和完整性。 该方法可以用于例如从衬底去除或分层材料，该材料响应于电场。 本发明还包括消除电场的应用和将先前去除的电场响应材料应用于衬底，从而恢复粘合剂结合的强度和完整性。 本发明包括用于键控制过程的装置。

公开(公告)号：US20080197025A1

公开(公告)日：2008-08-21

申请号：US11090944

申请日：2005-03-25

申请人： Yanxiu Zhou ,  Bin Yu ,  Kalle Levon

发明人： Yanxiu Zhou ,  Bin Yu ,  Kalle Levon

IPC分类号： G01N27/406

CPC分类号： C12Q1/6825 ,  C12Q2600/156 ,  G01N27/333 ,  C12Q2565/518

摘要： Ionic interactions are monitored to detect hybridization. The measurement may be done measuring the potential change in the solution with the ion sensitive electrode (which may be the conducting polymer (e.g., polyaniline) itself), without applying any external energy during the binding. The double helix formation during the complimentary hybridization makes this electrode act as an ion selective electrode—the nucleotide hydrogen bonding is specific and thus monitoring the ionic phosphate group addition becomes selective. Polyaniline on the surface of nylon film forms a positively charged polymer film. Thiol linkage can be utilized for polyaniline modification and thiol-modified single strand oligonucleotide chains can be added to polyaniline. The sensitivity is because the double helix formation during the complimentary hybridization makes this electrode act as an ion selective electrode as the nucleotide hydrogen bonding is specific and thus monitoring the ionic phosphate group addition becomes selective.

摘要翻译： 监测离子相互作用以检测杂交。 测量可以通过离子敏感电极（其可以是导电聚合物（例如聚苯胺）本身））测量溶液中的潜在变化，而在结合期间不施加任何外部能量。 在互补杂交期间的双螺旋形成使得该电极用作离子选择性电极 - 核苷酸氢键是特异性的，因此监测离子性磷酸酯基添加成为选择性的。 尼龙膜表面的聚苯胺形成带正电的聚合物膜。 硫醇连接可用于聚苯胺改性，硫醇改性的单链寡核苷酸链可以加入到聚苯胺中。 敏感性是因为互补杂交期间的双螺旋形成使得该电极作为离子选择性电极，因为核苷酸氢键是特异性的，因此监测离子性磷酸酯基添加成为选择性的。

公开(公告)号：US4610809A

公开(公告)日：1986-09-09

申请号：US694796

申请日：1985-01-25

申请人： Ermo Kaila ,  Anita Kinanen ,  Kalle Levon ,  Johannes Turunen ,  Jan-Erik sterholm ,  J. Johan Lindberg

发明人： Ermo Kaila ,  Anita Kinanen ,  Kalle Levon ,  Johannes Turunen ,  Jan-Erik sterholm ,  J. Johan Lindberg

IPC分类号： C08L101/00 ,  C08L97/00 ,  H01L39/12 ,  H01B1/00

CPC分类号： H01L39/121

摘要： A polymer that has been made of lignen and that contains sulphur, and into which a compound that activates the conductivity, such as iodine or ferric chloride, has been doped. The doped lignin polymer has properties of a semiconductor.

摘要翻译： 已经由木质素制成并含有硫的聚合物，其中已经掺杂了激活导电性的化合物，例如碘或氯化铁。 掺杂的木质素聚合物具有半导体的性质。

公开(公告)号：US20160003813A1

公开(公告)日：2016-01-07

申请号：US10888342

申请日：2004-07-09

申请人： Kalle Levon ,  Bin Yu ,  Yanxiu Zhou

发明人： Kalle Levon ,  Bin Yu ,  Yanxiu Zhou

IPC分类号： G01N33/543 ,  C09D183/04 ,  C07K17/08 ,  G01N33/545 ,  G01N27/327

CPC分类号： C12Q1/00 ,  C07K9/00 ,  C07K14/32 ,  C07K17/00 ,  C12Q1/003 ,  G01N33/53 ,  G01N33/54353 ,  G01N33/5438 ,  G01N33/54393 ,  G01N33/552

摘要： Sensors suitable for the sensing/detection of biological or chemical agents may be fabricated by immobilizing biological and/or chemical recognition components (selectors or probes) on a substrate by the polymerization of a suitable monomer in the presence of the selectors or probes, for example, by Polysiloxane Monolayer Immobilization (PMI). PMI may involve the polymerization of polysiloxane onto a substrate, onto which selector molecules are adsorbed or otherwise immobilized. The resulting immobilized selector molecule may then be used to interact with specific molecules (targets) within a mixture of molecules, thereby enabling those specific molecules to be detected and/or quantified.

摘要翻译： 适于感测/检测生物或化学试剂的传感器可以通过在选择器或探针的存在下通过合适的单体的聚合将生物和/或化学识别组分（选择器或探针）固定在基底上来制备，例如 ，通过聚硅氧烷单层固定（PMI）。 PMI可以涉及将聚硅氧烷聚合到底物上，在其上吸附或以其他方式固定选择分子。 然后可以使得到的固定化选择分子与分子混合物中的特定分子（靶）相互作用，从而使得能够检测和/或定量那些特异性分子。

公开(公告)号：US08961738B2

公开(公告)日：2015-02-24

申请号：US13570299

申请日：2012-08-09

申请人： Victor Barinov ,  Kalle Levon

发明人： Victor Barinov ,  Kalle Levon

IPC分类号： B32B38/10 ,  C09J5/00

CPC分类号： B32B43/006 ,  B32B37/12 ,  B32B38/00 ,  B32B2037/1246 ,  B32B2038/0052 ,  C09J5/00 ,  C09J2205/302 ,  Y10T29/49755 ,  Y10T29/49821 ,  Y10T156/11 ,  Y10T156/1158 ,  Y10T156/19

摘要： The invention concerns a method for controlling the adhesive bond between an electric-field responsive material and a substrate, which method comprises using an electric field to control the strength and integrity of the adhesive bond. The method may be used, for example, for removing or delaminating a material, which material is responsive to an electric field, from a substrate. The invention further comprises elimination of the application of the electric field and application of the previously removed electric-field-responsive material to the substrate, thereby resuming the strength and integrity of the adhesive bond. The invention includes apparatus for use in the bond controlling process.

摘要翻译： 本发明涉及一种用于控制电场响应材料和基底之间的粘合剂结合的方法，该方法包括使用电场来控制粘合剂粘结的强度和完整性。 该方法可以用于例如从衬底去除或分层材料，该材料响应于电场。 本发明还包括消除电场的应用和将先前去除的电场响应材料应用于衬底，从而恢复粘合剂结合的强度和完整性。 本发明包括用于键控制过程的装置。

公开(公告)号：US08342831B2

公开(公告)日：2013-01-01

申请号：US12296420

申请日：2007-04-09

申请人： Victor Barinov ,  Kalle Levon

发明人： Victor Barinov ,  Kalle Levon

IPC分类号： D01D5/08

CPC分类号： D01D5/0092 ,  D03D15/0061 ,  D03D41/00

摘要： An electrospinning apparatus for spinning polymer fibers from a fluid delivered by a jet supply device, which apparatus comprises at least one collector of a plurality of collectors in electrical communication with an electrode of the jet supply device. Collectors are insulated from each other. At least one collector comprises a stretcher adapted to stretch polymer fibers. The stretcher is an integral part of at least one collector. A controller controls sequence and time duration at which collectors are in electrical communication with the electrode of the jet supply device creating a non-woven polymer fabric or weaving polymer fibers into a fabric. The electrospinning apparatus comprises a rotator adapted to rotate the stretcher.

摘要翻译： 一种用于从由喷射供应装置输送的流体纺丝聚合物纤维的静电纺丝装置，该装置包括与喷气供应装置的电极电连通的多个收集器的至少一个收集器。 收集器彼此绝缘。 至少一个收集器包括适于拉伸聚合物纤维的拉伸机。 担架是至少一个收集器的组成部分。 控制器控制收集器与喷射供应装置的电极电连通的顺序和持续时间，从而产生非织造聚合物织物或将聚合物纤维编织成织物。 静电纺纱装置包括适于旋转担架的旋转器。