公开(公告)号：US20200248148A1

公开(公告)日：2020-08-06

申请号：US16616942

申请日：2018-05-23

Applicant: KYOTO UNIVERSITY

Inventor： Shinichi SUETA ,  Tomoko KASAHARA ,  Kenji OSAFUNE

IPC: C12N5/071 ,  C12N5/074

Abstract: A method of producing renal progenitor cells from pluripotent stem cells involves culturing pluripotent stem cells in a medium containing FGF2, BMP4, a GSK-3β inhibitor, and retinoic acid or a derivative thereof, culturing the resulting cells in a medium containing FGF2, a GSK-3β inhibitor, and BMP7, culturing the resulting cells in a medium containing FGF2, a GSK-3β inhibitor, BMP7, and a TGFβ inhibitor, culturing the resulting cells in a medium containing FGF2, a GSK-3β inhibitor, BMP7, activin, and a ROCK (Rho-kinase) inhibitor, culturing the resulting cells in a medium containing retinoic acid or a derivative thereof, and FGF9, and culturing the resulting cells in a medium containing a GSK-3β inhibitor and FGF9, to induce renal progenitor cells from intermediate mesodermal cells.

公开(公告)号：US20180119095A1

公开(公告)日：2018-05-03

申请号：US15539042

申请日：2015-12-25

Applicant: KYOTO UNIVERSITY

Inventor： Kenji OSAFUNE ,  Maki KOTAKA

IPC: C12N5/071 ,  G01N33/50

CPC classification number: C12N5/067 ,  C12N2500/30 ,  C12N2500/62 ,  C12N2501/12 ,  C12N2501/16 ,  C12N2501/237 ,  C12N2501/31 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/80 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5067

Abstract: A method for producing hepatocytes from hepatoblasts is provided. The method includes the step of culturing the hepatoblasts in a medium containing a compound selected from the group consisting of pregnenolone and an adrenergic agonist. The hepatoblasts can be obtained by culturing endodermal cells in a medium containing DMSO, and the endodermal cells can be obtained by culturing pluripotent stem cells in a medium containing Activin A and a GSK-3β inhibitor. Accordingly, a method for producing hepatocytes from pluripotent stem cells is also provided by employing the method of the present invention.

公开(公告)号：US20220380732A1

公开(公告)日：2022-12-01

申请号：US17765358

申请日：2020-09-30

Applicant: KYOTO UNIVERSITY

Inventor： Kenji OSAFUNE ,  Shinichi MAE ,  Makoto RYOSAKA

IPC: C12N5/071 ,  G01N33/569

Abstract: Provided is a method for isolating a ureteric bud tip cell from cells, a tissue, or an organoid comprising the ureteric bud tip cell, comprising the following steps of contacting the cells, tissue, or organoid comprising the ureteric bud tip cell with a very low density lipoprotein receptor (VLDL-R) binding agent, and isolating the ureteric bud tip cell using the binding agent as an indicator.

公开(公告)号：US20200010807A1

公开(公告)日：2020-01-09

申请号：US16461905

申请日：2017-11-21

Applicant: Kyoto University

Inventor： Kenji OSAFUNE ,  Satoshi MATSUI

IPC: C12N5/071

Abstract: Provided are: a method for producing biliary epithelial progenitor cells, said method comprising a step for culturing hepatoblasts in a medium containing TGFβ and EGF; and a method for constructing a three-dimensional duct-like structure of biliary epithelial progenitor cells, said method comprising a step for culturing hepatoblasts or biliary epithelial progenitor cells in a medium containing HGF, EGF, a Notch inhibitor and a GSK3 inhibitor in the presence of a three-dimensional scaffold material.

公开(公告)号：US20190390169A1

公开(公告)日：2019-12-26

申请号：US16490394

申请日：2018-03-02

Applicant: Kyoto University ,  Takeda Pharmaceutical Company Limited

Inventor： Kenji OSAFUNE ,  Taro TOYODA ,  Shiro TAKEKAWA ,  Goshi NAKAMURA ,  Ryo ITO

IPC: C12N5/071 ,  A61K35/545

Abstract: The present invention relates to a method for producing pancreatic progenitor cells from pluripotent stem cells. More specifically, the present invention relates to a method for producing pancreatic progenitor cells, comprising causing the action of a factor having the inhibitory activity for cyclin-dependent kinase 8 and/or cyclin-dependent kinase 19 (hereinafter, also abbreviated to CDK8/19).

公开(公告)号：US20180273905A1

公开(公告)日：2018-09-27

申请号：US15758580

申请日：2016-09-09

Applicant: Astellas Pharma Inc. ,  KYOTO UNIVERSITY

Inventor： Tatsuya KAWAMOTO ,  Yukiko YAMAGISHI ,  Kenji OSAFUNE

IPC: C12N5/071

Abstract: An object of the present invention resides in providing a method for acquiring and producing high-purity renal progenitor cells from a renal progenitor cell population into which pluripotent stem cells are induced to differentiate, by identifying a cell surface antigen marker specific to renal progenitor cells.A method for producing renal progenitor cells into which pluripotent stem cells are induced to differentiate, the method comprising the steps of:(i) culturing the pluripotent stem cells under conditions that induce differentiation into renal progenitor cells; and (ii) sorting a cell population from the cells obtained at step (i), by using at least one cell surface marker selected from the group consisting of CD9(−), CD55(−), CD106(+), CD140a(+), CD140b(+), CD165(+), CD271(+) and CD326(−).

公开(公告)号：US20170175082A1

公开(公告)日：2017-06-22

申请号：US15311759

申请日：2015-05-20

Applicant: Kyoto University

Inventor： Kenji OSAFUNE ,  Taro TOYODA

IPC: C12N5/071 ,  A61K35/39

CPC classification number: C12N5/0676 ,  A61K35/39 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  Y02A20/402

Abstract: Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1+/NKX6.1− cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.