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    DEVICE PREPARATION USING CONDENSED NUCLEIC ACID PARTICLES
    11.
    发明申请
    DEVICE PREPARATION USING CONDENSED NUCLEIC ACID PARTICLES 有权

    公开(公告)号：US20210214787A1

    公开(公告)日：2021-07-15

    申请号：US17217492

    申请日：2021-03-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Guobin LUO ,  Marina SEDOVA ,  David LIGHT ,  Ryan JONES ,  Mohammad ALANJARY

    IPC: C12Q1/6874 ,  C12Q1/6806

    Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.

    SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING
    15.
    发明申请
    SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING 审中-公开
    SCAFFEDED核酸聚合物颗粒及其制备和使用方法

    公开(公告)号：US20160194629A1

    公开(公告)日：2016-07-07

    申请号：US14987552

    申请日：2016-01-04

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Wolfgang HINZ ,  John LEAMON ,  David LIGHT ,  Jonathan M. ROTHBERG

    IPC: C12N15/10 ,  C12Q1/68 ,  B01J19/00

    CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

    Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

    Abstract translation: 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

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